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Effect of storage of domestic cat (Felis catus) epididymides at 5°C on sperm quality and cryopreservationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, best results are achieved when sperm are recovered from epididymides within 24h of cool storage and when they are equilibrated with glycerol during 120min before freezing. The current results should be useful in the further development of techniques for the rescue and cryostorage of epididymal spermatozoa of endangered felids. (Source: Theriogenology)
Source: Theriogenology - November 2, 2009 Category: Genetics & Stem Cells Authors: N. Gañán, M. Gomendio, E.R.S. Roldan Tags: Research articles Source Type: journals

Comparison of accuracy of ultrasonography, progesterone, and pregnancy-associated glycoprotein tests for pregnancy diagnosis in semidomesticated reindeerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The aim of the study was to compare transrectal ultrasound with progesterone (P4) and pregnancy-associated glycoproteins (PAGs) as pregnancy detection methods for semidomesticated reindeer (Rangifer tarandus tarandus) in field conditions. Female reindeer (n=195) were scanned transrectally by a 7.5-MHz linear array transducer, and blood was sampled either in December 2005 (n=33), December 2006 (n=92), or January 2007 (n=70) during early or mid gestation. Plasma levels of P4 and PAGs were assessed by radioimmunoassay (RIA). Based on calving records, the sensitivity, specificity, predictive values, and the overall a...
Source: Theriogenology - November 2, 2009 Category: Genetics & Stem Cells Authors: H. Savela, S. Vahtiala, H. Lindeberg, E. Dahl, E. Ropstad, J-F. Beckers, S. Saarela Tags: Research articles Source Type: journals

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Source: Theriogenology - November 2, 2009 Category: Genetics & Stem Cells Source Type: journals

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(Source: Theriogenology)
Source: Theriogenology - November 2, 2009 Category: Genetics & Stem Cells Source Type: journals

Follicle population, cumulus mucification, and oocyte chromatin configuration during the periovulatory period in the female dogEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to describe the follicular population present on the canine ovary (Canis familiaris) during the preovulatory period and essentially the changes in oocyte size, mucification, and chromatin configuration occurring from before the luteinizing hormone (LH) surge up to postovulation. In a first experiment, ovaries of beagle bitches were collected before (n=21) or after LH surge but before ovulation (post-LH surge/preovulation stage, n=24) as determined using hormone (LH, estradiol, progesterone) assays and ultrasonography. All large (>2mm) follicles were measured and punctured. The numbers of oocytes col...
Source: Theriogenology - October 5, 2009 Category: Genetics & Stem Cells Authors: K. Reynaud, C. Viaris de Lesegno, M. Chebrout, S. Thoumire, S. Chastant-Maillard Tags: Research articles Source Type: journals

Effects of exogenous progesterone and cloprostenol on ovarian follicular development and first ovulation in prepubertal heifersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to determine the effects of progesterone and cloprostenol (a PGF2α analogue) on ovarian follicular development and ovulation in prepubertal heifers. In Experiment 1, crossbred Hereford heifers (Bos taurus; 10 to 12 mo old, 255 to 320kg) were assigned randomly to three groups and given (1) an intravaginal progesterone-releasing insert (CIDR; P group, n=13); (2) a CIDR plus 500μg cloprostenol im (PGF2α analogue) at CIDR removal (PPG group, n=11); or (3) no treatment (control group, n=14). The CIDR inserts were removed 5 d after follicular wave emergence. Progesterone-treated heifers (P and ...
Source: Theriogenology - October 5, 2009 Category: Genetics & Stem Cells Authors: L.F.M. Pfeifer, L.G. Siqueira, R.J. Mapletoft, J.P. Kastelic, G.P. Adams, M.G. Colazo, J. Singh Tags: Research articles Source Type: journals

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Source: Theriogenology - October 5, 2009 Category: Genetics & Stem Cells Source Type: journals

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Source: Theriogenology - October 5, 2009 Category: Genetics & Stem Cells Source Type: journals

Influences of diet during gestation on potential postpartum reproductive performance and milk production of beef heifersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The influences of nutritional protein and energy during early and mid pregnancy on milk production and postpartum reproductive parameters were determined in 70 beef heifers of two composite breeds (Bos indicus X Bos taurus). At artificial insemination (AI), heifers were divided into four dietary treatment groups identified by the level of protein, and to a lesser extent energy, fed during the first and second trimesters: high/high (HH), high/low (HL), low/high (LH), and low/low (LL). Milk production was lower in the heifers receiving high treatment in first trimester than that in heifers receiving the low treatme...
Source: Theriogenology - October 5, 2009 Category: Genetics & Stem Cells Authors: T.M. Sullivan, G.C. Micke, V.E.A. Perry Tags: Research articles Source Type: journals

Effect of oxytocin and flunixin meglumine on uterine response to insemination in maresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The most probable reason for persistent postbreeding endometritis in mares is weak myometrial contractility. The influence of oxytocin (OT; an ecbolic agent) and flunixin meglumine (FLU; a prostaglandin inhibitor serving as a model for mares with decreased uterine contractility) on uterine response to artificial insemination (AI) was studied in mares with no history of reproductive failure. The mares were treated intravenously with 10mL saline (Group C, n=10) or 0.01 IU/kg OT (Group OT, n=10) 2, 4, 8, and 25h after AI. Group FLU (n=11) was treated with 1.1mg/kg FLU 2h after AI and with saline thereafter. The mare...
Source: Theriogenology - September 28, 2009 Category: Genetics & Stem Cells Authors: A.M. Risco, T. Reilas, L. Muilu, M. Kareskoski, T. Katila Tags: Research articles Source Type: journals

Sperm quality evaluation in Solea senegalensis during the reproductive season at cellular levelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Sperm quality seems to be one of the reasons for the reproduction constraints faced by Senegalese sole (Solea senegalensis) aquaculturists. Previous studies in this species indicated that the sperm quality of individuals kept in culture varies throughout the year and that different sperm subpopulations can be identified in ejaculates according to the motility pattern of spermatozoa. Aiming to better understand factors affecting sole sperm quality in captivity, sperm of 11 males was assessed during the reproductive season using different parameters: motility characteristics using CASA analysis; cell plasma membran...
Source: Theriogenology - September 25, 2009 Category: Genetics & Stem Cells Authors: J. Beirão, F. Soares, M.P. Herráez, M.T. Dinis, E. Cabrita Tags: Research articles Source Type: journals

Effects of low versus physiologic plasma progesterone concentrations on ovarian follicular development and fertility in beef cattleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to determine the effects of low versus physiologic plasma progesterone concentrations during the ovulatory wave on fertility in cattle. Suckled beef cows (Bos taurus; n=129) and pubertal heifers (Bos taurus; n=150) at random stages of the estrous cycle were given a luteolytic dose of prostaglandin F2α (500μg cloprostenol; PGF) twice, 11 d apart. Ten days after the second PGF treatment, cattle were given estradiol benzoate im (1.5 and 1.0mg for cows and heifers, respectively) and a progesterone-releasing intravaginal device (Cue-Mate) with a single pod containing 0.78g progesterone (Day 0)....
Source: Theriogenology - September 25, 2009 Category: Genetics & Stem Cells Authors: L.F.M. Pfeifer, R.J. Mapletoft, J.P. Kastelic, J.A. Small, G.P. Adams, N.J. Dionello, J. Singh Tags: Research articles Source Type: journals

Association between evaluation of the reproductive tract by various diagnostic tests and restoration of ovarian cyclicity in high-producing dairy cowsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The uterine condition of clinically normal postpartum Holstein-Friesian dairy cows (n = 45) was evaluated once weekly (Weeks 3 to 7) by endometrial cytology, vaginal mucus collection device (VMCD), vaginoscopy, and ultrasonography to establish a relationship with postpartum resumption of ovulatory cycles. The time of first detection of the corpus luteum (CL) by ultrasonography and plasma progesterone concentration ≥1 ng/mL was recorded. By 49 d postpartum, 78% of the cows (n=35) had resumed ovarian function (CL group), whereas the remainder (n=10) had no CL (NCL group). There was a positive correlation between ...
Source: Theriogenology - September 25, 2009 Category: Genetics & Stem Cells Authors: W.S. Senosy, M. Uchiza, N. Tameoka, Y. Izaike, T. Osawa Tags: Research articles Source Type: journals

Measuring fecal progestogens as a tool to monitor reproductive activity in captive female bottlenose dolphins (Tursiops truncatus)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective was to develop and test radioimmunoassays (RIAs) to measure fecal progestogens (P) and estrogens (E) to monitor ovarian activity in the bottlenose dolphin (Tursiops truncatus). Fecal samples were collected at least once a week for 20 mo from three peripubertal female bottlenose dolphins. Blood samples were collected at least once a month to compare serum and fecal steroid concentrations. Moreover, random fecal samples from three pregnant females, one lactating female, and one sexually mature female receiving oral altrenogest treatment were also collected. Fecal samples were collected behaviorally with a probe...
Source: Theriogenology - September 24, 2009 Category: Genetics & Stem Cells Authors: B. Biancani, L. Da Dalt, G. Lacave, S. Romagnoli, G. Gabai Tags: Research articles Source Type: journals

The effect of hormone treatments (hCG and cloprostenol) and season on the incidence of hemorrhagic anovulatory follicles in the mare: A field studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The association between use of hormone treatments to induce estrus and ovulation and the incidence of hemorrhagic anovulatory follicles (HAFs) was studied in a mixed population of mares (Equus caballus) during two breeding seasons in a commercial breeding clinic. Mares treated with cloprostenol (CLO) were more likely to develop HAFs than were mares with spontaneous cycles (P0.05) from that of mares with ovulatory cycles (10.5±1.5 yr). (Source: Theriogenology)
Source: Theriogenology - September 24, 2009 Category: Genetics & Stem Cells Authors: J. Cuervo-Arango, J.R. Newcombe Tags: Research articles Source Type: journals

Mycoplasma agalactiae detected in the semen of goat bucksEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to detect the excretion in semen of CA-causing mycoplasmas in goats (Capra hircus) reared in Spain, where the disease is considered endemic. Culture techniques and PCR were conducted on 147 semen samples collected from 113 goat bucks to detect mycoplasmas. No animal showed clinical symptoms of CA at the moment of the screening. M. agalactiae was identified using both diagnostic methods in three semen samples collected from three different bucks. These animals belonged to a group of animals in which semen had been analyzed twice and only the second sample proved positive, suggesting the possibility o...
Source: Theriogenology - September 23, 2009 Category: Genetics & Stem Cells Authors: C. de la Fe, J. Amores, A. Gómez Martín, A. Sánchez, A. Contreras, J.C. Corrales Tags: Research articles Source Type: journals

Induction of lactational estrus in organic piglet productionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to investigate the occurrence and timing of lactational estrus in an organic outdoor system based on ad libitum feeding, individual housing until Day 35 in lactation, followed by grouping and introduction of a boar and weaning of piglets after 8 wk. Five groups with four sows ((Danish Yorkshire×Danish Landrace)×Danish Duroc) in each were observed, and rank was determined by a food competition test. All sows showed lactational estrus, and 84% of these sows showed estrus within 1 wk, on average 43.5 d and 7.3 d after farrowing and boar introduction, respectively. The number of days from boar...
Source: Theriogenology - September 23, 2009 Category: Genetics & Stem Cells Authors: A.G. Kongsted, J.E. Hermansen Tags: Research articles Source Type: journals

Freemartinism and FecXR allele determination in replacement ewes of the Rasa Aragonesa sheep breed by duplex PCREmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: A new naturally occurring mutation in the fecundity gene BMP15 in the Rasa Aragonesa sheep breed (Ovis aries) has been found to affect prolificacy. This mutation (FecXR allele) is a deletion of 17 base pairs that leads to an altered amino acid sequence, and this alteration increases prolificacy in heterozygous ewes but causes sterility in homozygous ewes. Selection of repository lambs with the FecXR allele increases rates of twins and multiple lambing and thereby also increases the probability of lambing freemartins that will become sterile. In this sense, an accurate, reliable, and quick method was developed by ...
Source: Theriogenology - September 22, 2009 Category: Genetics & Stem Cells Authors: A. Martinez-Royo, E. Dervishi, J.L. Alabart, J.J. Jurado, J. Folch, J.H. Calvo Tags: Technical note Source Type: journals

Optimization of the cryopreservation of African clawed frog (Xenopus laevis) spermEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, dilution of collected sperm in MIS solution (to have a final concentration of 6.5×106 to 8×106/mL) containing 5% DMSO and 73 mmol/L sucrose, freezing in a vapor of liquid nitrogen at 10cm above the surface, and thawing at room temperature for 40sec was the best cryopreservation protocol. This protocol gave 70% hatching rate, 80% motility rate, and 75% viability rate of fresh hormonally induced sperm. (Source: Theriogenology)
Source: Theriogenology - September 22, 2009 Category: Genetics & Stem Cells Authors: N. Mansour, F. Lahnsteiner, R.A. Patzner Tags: Research articles Source Type: journals

The influence of cryopreservation and seminal plasma on the chromatin structure of dog spermatozoaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: It was the aim of the current study to investigate effects of seminal plasma on the chromatin structure of frozen-thawed canine (Canis lupus familiaris) spermatozoa. A total of 20 ejaculates were collected. Ejaculates were divided, and one half was centrifuged for removal of seminal plasma (c) while the other was left uncentrifuged (nc) before cryopreservation. This was performed according to the Uppsala system in a computerized freezing machine. Before freezing (bf) and after thawing (at), samples were investigated for motility (M), viability (CASA), and chromatin status (sperm chromatin structure assay; SCSA). ...
Source: Theriogenology - September 22, 2009 Category: Genetics & Stem Cells Authors: M. Koderle, C. Aurich, S. Schäfer-Somi Tags: Research articles Source Type: journals

Sperm-mediated gene transfer–treated spermatozoa maintain good quality parameters and in vitro fertilization ability in swineEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: A simple and efficient method for producing multitransgenic animals is required for medical and veterinary applications. Sperm-mediated gene transfer (SMGT) is an effective method for introducing multiple genes into pigs (Sus, Sus scrofa). The major benefits of this technique are the high efficiency, low cost, and ease of use compared with that of other methods: Sperm-mediated gene transfer does not require embryo handling or expensive equipment. The aim of this study was to investigate the influence of SMGT treatment and exogenous DNA uptake on sperm quality. Even after a coincubation with a 20-fold larger amoun...
Source: Theriogenology - September 22, 2009 Category: Genetics & Stem Cells Authors: M.L. Bacci, A. Zannoni, M. De Cecco, P. Fantinati, C. Bernardini, G. Galeati, M. Spinaci, R.Giovannoni, M. Lavitrano, E. Seren, M. Forni Tags: Research articles Source Type: journals

Cholesterol concentration in seminal plasma as a predictive tool for quality semen evaluationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The aim of this study was to investigate the relationship between lipid composition of bovine serum and seminal plasma, seasonality, and semen quality. The experiment was carried out in two groups of Simmental breeding bulls: Group I (ages 2 to 4 yr) and Group II (ages 5 to 10 yr). Blood samples were collected from jugular vein, and bovine semen was sampled with an artificial vagina once per season. Serum concentrations of total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triacylglycerols, nonesterified fatty acids (NEFAs), and lipoprotein electrophoreti...
Source: Theriogenology - September 21, 2009 Category: Genetics & Stem Cells Authors: B. Beer-Ljubić, J. Aladrović, T.S. Marenjak, R. Laškaj, I. Majić-Balić, S. Milinković-Tur Tags: Research articles Source Type: journals

Assessment of plasma membrane and chromatin structure of sperm from transgenic and non-transgenic boarsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the presence of the human α1,2-fucosyltransferase gene in the genome of TG boars did not cause any spermatogenesis process disturbances leading to the increased production of apoptotic spermatozoa. Moreover, the low level of sperm with damaged chromatin in TG boars confirms the high stability of the spermatogenesis process in the TG boars analyzed. (Source: Theriogenology)
Source: Theriogenology - September 17, 2009 Category: Genetics & Stem Cells Authors: M. Trzcińska, M. Bryła, M. Bochenek, R. Słomski, Z. Smorąg Tags: Research articles Source Type: journals

Trichostatin A affects histone acetylation and gene expression in porcine somatic cell nucleus transfer embryosEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Epigenetic aberrancies likely preclude correct and complete nuclear reprogramming after somatic cell nucleus transfer (SCNT) and may underlie the observed reduced viability of cloned embryos. In the current study, we tested the effects of the histone deacetylase-inhibitor trichostatin A (TSA) on preimplantation development and on histone acetylation and the gene expression of nucleus transfer (NT) porcine (Sus scrofa) embryos. Our results showed that 5nM TSA for 26h after reconstitution resulted in embryos (NTTSA) that reached the blastocyst stage at a higher level (48.1% vs. 20.2%) and increased number of cells ...
Source: Theriogenology - September 17, 2009 Category: Genetics & Stem Cells Authors: R.P. Cervera, N. Martí-Gutiérrez, E. Escorihuela, R. Moreno, M. Stojkovic Tags: Research articles Source Type: journals

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Source: Theriogenology - September 17, 2009 Category: Genetics & Stem Cells Source Type: journals

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Source: Theriogenology - September 17, 2009 Category: Genetics & Stem Cells Source Type: journals

Plasma PGF2α metabolite levels in cats with uterine diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objectives in this study were to investigate PGFM levels, presence of SIRS, and clinical and laboratory parameters in female cats as possible indicators for severity of uterine disease. In total, 7 female cats with pyometra, 2 with mucometra, 7 with cystic endometrial hyperplasia (CEH), and 14 healthy control cats were included. Physical examination, ovariohysterectomy, and histopathology were performed, laboratory parameters were analyzed, and PGFM levels were analyzed by radioimmunoassay. Analysis of variance, Fisher's exact test, Student's t-test and Pearson's product moment correlation coefficient were used for dat...
Source: Theriogenology - September 14, 2009 Category: Genetics & Stem Cells Authors: R. Hagman, E. Karlstam, S. Persson, H. Kindahl Tags: Research articles Source Type: journals

Concentrations of 15-ketodihydro-PGF2α, cortisol, and progesterone in the plasma of healthy and pathologic newborn foalsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Information regarding the plasma hormone profiles of prostaglandins (PGs), cortisol (C), and progesterone (P4) during pathologic processes in newborn foals is scarce. The aim of this study was to determine the plasma concentrations of these hormones in diseased foals (n=40) and healthy at-term foals (n=24) (Equus caballus) during the first 2 weeks of life. Blood samples were collected daily, before any treatment with nonsteroidal drugs in diseased foals, and plasma was analyzed by radioimmunoassay. 15-Ketodihydro-PGF2α (PGM) was consistently higher in diseased foals than in healthy foals, probably related to rol...
Source: Theriogenology - September 13, 2009 Category: Genetics & Stem Cells Authors: S. Panzani, M. Villani, A. McGladdery, M. Magri, H. Kindahl, G. Galeati, P.A. Martino, M.C. Veronesi Tags: Research articles Source Type: journals

Effects of dilution and centrifugation on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey semenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The aim of this work was to study the effects of dilution and centrifugation (i.e., two methods of reducing the influence of the seminal plasma) on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey (Equus asinus) semen. Fifty ejaculates from nine Catalonian jackasses were collected. Gel-free semen was diluted 1:1, 1:5 or 1:10 with Kenney extender. Another sample of semen was diluted 1:5, centrifuged, and then resuspended with Kenney extender until a final dilution of 25×106 sperm/ml was achieved (C). After 24h, 48h or 72h of refrigerated storage a...
Source: Theriogenology - September 10, 2009 Category: Genetics & Stem Cells Authors: J. Miró, E. Taberner, M. Rivera, A. Peña, A. Medrano, T. Rigau, A. Peñalba Tags: Research articles Source Type: journals

Differing sperm ability to penetrate the oocyte in vivo and in vitro as revealed using colloidal preparationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The penetration ability of boar (Sus scrofa domestica) spermatozoa exposed to viscous preparations under in vivo and in vitro fertilization conditions has been examined. Experiments involving induced ovulation in prepubertal animals and surgical insemination directly into the oviduct isthmus revealed an advantage of colloidal preparations. Based on within-animal comparisons, the incidence of penetration was 100% using both spermatozoa suspended in a viscous preparation of plant extracts and spermatozoa suspended in a control medium. However, percentages of monospermy were 22.2% in 54 oocytes inseminated with the ...
Source: Theriogenology - September 10, 2009 Category: Genetics & Stem Cells Authors: P. Coy, J. Gadea, D. Rath, R.H.F. Hunter Tags: Research articles Source Type: journals

Artificial insemination of captive European brown hares (Lepus europaeus PALLAS, 1778) with fresh and cryopreserved semen derived from free-ranging malesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to establish artificial insemination (AI) protocols to predictably initiate pregnancy during the breeding season in the European brown hare (EBH) (Lepus europaeus PALLAS, 1778). Semen was collected from seven captive and eight free-ranging males by means of electroejaculation. Semen from the free-ranging males was cryopreserved using directional freezing. Total motility/integrity of fresh and frozen-thawed semen was 91.6%/87.7% and 46.9%/53.8%, respectively. Ovulation was induced in ultrasonographically preselected females using a gonadotropin-releasing hormone analogue. Each female was inseminated with 1m...
Source: Theriogenology - September 8, 2009 Category: Genetics & Stem Cells Authors: T.B. Hildebrandt, K. Roellig, F. Goeritz, M. Fassbender, R. Krieg, S. Blottner, B. Behr, R. Hermes Tags: Research articles Source Type: journals

Concentrations of circulating hormones normalized to pulses of a prostaglandin F2α metabolite during spontaneous luteolysis in maresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The temporal relationships between a pulse of 13,14-dihydro-15-keto-PGF2α (PGFM) and the concentrations of circulating hormones during the luteolytic period were studied for 11 pulses in 11 mares (Equus caballus) using samples collected hourly. Mean PGFM pulses encompassed 4h before to 4h after the peak, and hormone data were normalized to the PGFM peak (Hour 0). Concentration of progesterone decreased (P < 0.05) between Hours –4 and –3 and continued to decrease linearly throughout the PGFM pulse. The concentrations of cortisol and prolactin increased (P < 0.004) during Hours –4 to 0 and decreased (P < 0.0...
Source: Theriogenology - September 6, 2009 Category: Genetics & Stem Cells Authors: O.J. Ginther, M.A. Beg Tags: Research articles Source Type: journals

Washing increases the susceptibility to exogenous oxidative stress in red deer spermatozoaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The effects of routine sperm work are often overlooked. We assessed the effect of washing cryopreserved epididymal spermatozoa from red deer (Cervus elaphus hispanicus, Helzheimer 1909). After thawing, epididymal samples (four stags) were diluted in TALP-HEPES. A split was left untouched, another was centrifuged (300×g, 5min) and resuspended, and a third was centrifuged and the supernatant substituted by fresh TALP-HEPES (washing). Each split was supplemented either with nothing, 1mM of the antioxidant Trolox, 100μM of the oxidant Fe (with ascorbate), or both. The 3×4 treatments were incubated at 37°C and ass...
Source: Theriogenology - September 6, 2009 Category: Genetics & Stem Cells Authors: Á.E. Domínguez-Rebolledo, M.R. Fernández-Santos, O. García-Álvarez, A. Maroto-Morales, J.J. Garde, F. Martínez-Pastor Tags: Research articles Source Type: journals

Evaluation of DNA fragmentation of freeze-dried mouse sperm using a modified sperm chromatin structure assayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Freeze-dried sperm is applicable to the storage and transport of genetic material. We recently reported that freeze-dried mouse sperm required temperatures lower than −80°C for long-term preservation and concluded that it was necessary to explore freeze-drying conditions before long-term preservation of sperm becomes viable. In the current study, we determined the percentage of sperm with elevated levels of DNA fragmentation using a sperm chromatin structure assay (SCSA), a technique not previously reported for the evaluation of freeze-dried mouse sperm. We applied SCSA to mouse sperm freeze-dried under four c...
Source: Theriogenology - September 3, 2009 Category: Genetics & Stem Cells Authors: Y. Kawase, N.A. Wada, K. Jishage Tags: Research articles Source Type: journals

Amphiregulin promotes the proliferation of trophoblast cells during preimplantation development of porcine embryosEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Our objective was to investigate the effects of in vitro culture (IVC) medium supplemented with amphiregulin (AREG) on the preimplantation embryonic development of porcine (Genus: Sus domestica, Species: Landrace) embryos derived from in vitro fertilization (IVF) and parthenogenetic activation (PA). In vitro fertilization and PA embryos at the 1-cell stage were cultured in IVC medium supplemented with 0, 0.5, 5, or 50 ng/mL AREG for 7 d. There were significantly greater total numbers of cells in blastocysts of IVF and PA embryos cultured with 50 ng/mL AREG compared with that of controls. In vitro fertilization an...
Source: Theriogenology - September 3, 2009 Category: Genetics & Stem Cells Authors: J.H. Lee, E. Lee, D. Biswas, C.S. Jeung, G.S. Lee, S.H. Hyun, E.B. Jeung Tags: Research articles Source Type: journals

Antibody-based diagnosis of small ruminant lentivirus infection in seminal fluidEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Antibody-based diagnosis of small ruminant lentiviruses (SRLVs) has been efficiently achieved using serum and milk, but not semen, for which polymerase chain reaction (PCR) has been proposed as a confirmatory technique. This work, involving 296 ovine (Ovis aries) and caprine (Capra hircus) semen donors, investigates whether seminal fluid (SF) can be reliably used in antibody-based SRLV diagnosis. First, a gold standard was established to assess the infection status and determine the sensitivity and specificity of three commercial enzyme-linked immunosorbent assays (ELISAs) in serum testing using Western blot and ...
Source: Theriogenology - September 2, 2009 Category: Genetics & Stem Cells Authors: H. Ramírez, B. San Román, I. Glaria, R. Reina, M.M. Hernández, X. de Andrés, H. Crespo, B. Hichou, S. Cianca, C. Goñi, A. Grandas, L. García-Pastor, L.E. Vijil, F. Quintín, M.J. Grilló, D. de Andrés, B. Amorena Tags: Research articles Source Type: journals

Dynamics of heat shock protein 70 concentrations in peripheral blood lymphocyte lysates during pregnancy in lactating Holstein-Friesian cowsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The aim of this study was to characterize the dynamics of the concentrations of heat shock protein 70kDa (HSP70) in peripheral blood lymphocytes of lactating Holstein-Friesian dairy cows (Bos taurus) during pregnancy. The detection of pregnancy was carried out and blood samples collected on Days 40, 90, 120, 150, 180, and 210 of gestation from 46 cows (11 primiparous and 35 pluriparous, 34 seropositive and 12 seronegative to Neospora caninum). Peripheral blood lymphocytes were isolated by density gradient centrifugation. Serologic analysis of Neospora infection and determinations of HSP70 concentrations in lympho...
Source: Theriogenology - September 2, 2009 Category: Genetics & Stem Cells Authors: J.L. Yániz, F. López-Gatius, S. Almería, T. Carretero, I. García-Ispierto, B. Serrano, R.F. Smith, H. Dobson, P. Santolaria Tags: Research articles Source Type: journals

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(Source: Theriogenology)
Source: Theriogenology - September 2, 2009 Category: Genetics & Stem Cells Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Theriogenology)
Source: Theriogenology - September 2, 2009 Category: Genetics & Stem Cells Source Type: journals

Insemination timing affects pregnancy rates in beef cows treated with CO-Synch protocol including an intravaginal progesterone insertEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Our objective was to determine the optimal time to artificially inseminate lactating beef cows (Bos taurus typicus) after using the standard CO-Synch protocol that also included a progesterone-releasing, intravaginal controlled internal drug release (CIDR) insert. Cows (N=605) at three locations were inseminated at four different times after CIDR insert removal and the prostaglandin F2α administration of the CO-Synch+CIDR protocol: 48, 56, 64, or 72h. Blood samples were collected 9 to 10 d before and on the day of CIDR insertion. Based on elevated (>1 ng/mL) serum progesterone concentrations, 60% of 605 cows had...
Source: Theriogenology - September 1, 2009 Category: Genetics & Stem Cells Authors: C.A. Dobbins, D.R. Eborn, D.E. Tenhouse, R.M. Breiner, S.K. Johnson, T.T. Marston, J.S. Stevenson Tags: Research articles Source Type: journals

Validation of a new pregnancy-associated glycoprotein radioimmunoassay method for the detection of early pregnancy in ewesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The aim of the current study was to describe the use of a pool of different antisera raised against pregnancy-associated glycoproteins (PAGs; purified from both ovine and caprine placentas) for early pregnancy diagnosis in ovine species. Sixty-three pluriparous Sarda ewes (Ovis aries) were synchronized. Blood samples were withdrawn on Days 18, 24, 26, 28, 30, and 50 after mating. These samples were assayed for progesterone (radioimmunoassay [RIA] including an extraction step) and for pregnancy-associated glycoproteins (RIA-706 and RIA-srPool). Progesterone concentrations were under 1.0ng/mL in all nonpregnant Sar...
Source: Theriogenology - September 1, 2009 Category: Genetics & Stem Cells Authors: O. Barbato, N.M. Sousa, A. Debenedetti, C. Canali, L. Todini, J.F. Beckers Tags: Research articles Source Type: journals

In vitro characteristics of frozen-thawed, sex-sorted bull sperm after refreezing or incubation at 15 or 37 °CEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective was to determine the in vitro characteristics of frozen-thawed dairy bull sperm after sex-sorting and refreezing and thawing (0, 2, and 4h post-thaw; 37°C) or post-sort incubation at 15 or 37°C for 30 and 24h, respectively. These sperm were compared with nonsorted frozen-thawed sperm (control) and with nonsorted sperm undergoing two cryopreservation procedures (FF; 0, 2, and 4h). Frozen-thawed sex-sorted (FS) sperm maintained at 15 or 37°C had higher (P (Source: Theriogenology)
Source: Theriogenology - August 31, 2009 Category: Genetics & Stem Cells Authors: S.L. Underwood, R. Bathgate, W.M.C. Maxwell, G. Evans Tags: Research articles Source Type: journals

Evaluation of models to induce low progesterone during the early luteal phase in cattleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Two experiments were designed to evaluate models for generation of low circulating progesterone concentrations during early pregnancy in cattle. In Experiment 1, 17 crossbred heifers (Bos taurus) were assigned to either prostaglandin F2α (PGF2α) administration on Days 3, 3.5, and 4 (PG3; n=9) or to control (n=8). Blood samples were collected from heifers from Days 1 to 9 for progesterone assay. Progesterone concentrations were decreased (P (Source: Theriogenology)
Source: Theriogenology - August 31, 2009 Category: Genetics & Stem Cells Authors: M.E. Beltman, J.F. Roche, P. Lonergan, N. Forde, M.A. Crowe Tags: Research articles Source Type: journals

Ovarian superstimulation, transrectal ultrasound-guided oocyte recovery, and IVF in rhinocerosEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here on superstimulation, repeated oocyte recovery, and attempted in vitro fertilization (IVF) in white and black rhinoceroses. Four anestrous rhinoceroses (two white, two black) with unknown follicular status were treated with gonadotropin-releasing hormone analogue, deslorelin acetate, for 6 to 7 d. Number and size of follicles in superstimulated females was significantly higher and larger compared with those in nonstimulated anestrous females (n=9). Ovum pick-up was achieved by transrectal ultrasound-guided follicle aspiration. Up to 15 follicles were aspirated per ovary. During six ovum pick-ups, a total of 2...
Source: Theriogenology - August 31, 2009 Category: Genetics & Stem Cells Authors: R. Hermes, F. Göritz, T.J. Portas, B.R. Bryant, J.M. Kelly, L.J. Maclellan, T. Keeley, F. Schwarzenberger, C. Walzer, A. Schnorrenberg, R.E. Spindler, J. Saragusty, S. Kaandorp, T.B. Hildebrandt Tags: Research articles Source Type: journals

Genetic parameters for oocyte number and embryo production within a bovine ovum pick-up–in vitro production embryo-production programEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study revealed OPU-IVP traits (NembD7, NTembD7, and Ncoc) that could be of potential value for selection. Introduction of such traits in breeding programs would enhance the number of offspring from superior donors as well as improve the cost efficiency of OPU-IVP programs. (Source: Theriogenology)
Source: Theriogenology - August 27, 2009 Category: Genetics & Stem Cells Authors: J.S. Merton, B. Ask, D.C. Onkundi, E. Mullaart, B. Colenbrander, M. Nielen Tags: Research articles Source Type: journals

Pregnancy rates and corpus luteum–related factors affecting pregnancy establishment in bovine recipients synchronized for fixed-time embryo transferEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, corpus luteum–related traits were poor predictors of pregnancy in recipients. The type of embryo, however, was a major factor affecting pregnancy outcome. (Source: Theriogenology)
Source: Theriogenology - August 25, 2009 Category: Genetics & Stem Cells Authors: L.G.B. Siqueira, C.A.A. Torres, E.D. Souza, P.L.J. Monteiro, E.K.N. Arashiro, L.S.A. Camargo, C.A.C. Fernandes, J.H.M. Viana Tags: Research articles Source Type: journals

Characteristics of cryptic/ectopic and contralateral scrotal testes in dogs between 1 and 2 years of ageEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Testicular malposition represents a common developmental genital defect in dogs and can affect one or both testes. In both humans and dogs, unilateral cryptorchism is more frequently detected and thought to be the expression of a genetic abnormality affecting both the undescended and scrotal testis. In the dog, there is evidence of degenerative processes affecting the maldescended testis. However, the histologic and functional changes that occur in the scrotal testis of unilateral cryptorchid or ectopic individuals remain a source of debate. Because the bilateral surgical removal of the testes leads to some undes...
Source: Theriogenology - August 24, 2009 Category: Genetics & Stem Cells Authors: M.C. Veronesi, E. Riccardi, A. Rota, V. Grieco Tags: Research articles Source Type: journals

Factors affecting laboratory production of buffalo embryos: A meta-analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: In vitro fertilization (IVF) provides an excellent and inexpensive source of embryos for carrying out basic research on developmental physiology, farm animal breeding, and for commercial applications. Meta-analysis of the results from different publications rather than a narrative review may provide a current status of this technology in buffalo (Bubalus bubalis). In order to gain an idea of the factors affecting the IVF in buffalo, a review of the various studies conducted on buffalo IVF and a meta-analysis of their findings was undertaken. More than 100 articles published from 1991 to 2008 were searched, and re...
Source: Theriogenology - August 19, 2009 Category: Genetics & Stem Cells Authors: K.P. Suresh, S. Nandi, S. Mondal Tags: Research articles Source Type: journals

ContentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Theriogenology)
Source: Theriogenology - August 18, 2009 Category: Genetics & Stem Cells Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Theriogenology)
Source: Theriogenology - August 18, 2009 Category: Genetics & Stem Cells Source Type: journals