Characterization of the intestinal transport mechanism of polystyrene microplastics (MPs) and the potential inhibitory effect of green tea extracts on MPs intestinal absorption
Toxicol In Vitro. 2024 Mar 24;97:105813. doi: 10.1016/j.tiv.2024.105813. Online ahead of print.ABSTRACTThe aims of the current study included characterizing the intestinal transport mechanism of polystyrene microplastics (MPs) with different charges and sizes in the intestinal epithelial cell model and determining the inhibitory effect of green tea extracts (GTEs) on the intestinal absorption of MPs in Caco-2 cells. The smaller sizes, which included diameters of 0.2 μm, of amine-modified MPs compared to either larger size (1 μm diameter, or carboxylate-MPs (0.2 and 1 μm diameter) significantly lowered the cell viability...
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Woo-Hyun Kim Dong-Ho Lee Jeong-Eun Kim Hyun Woo Jeong Jin-Oh Chung JongHwa Roh WanGi Kim Xiaoting Fu Soon-Mi Shim Source Type: research

In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research

Characterization of the intestinal transport mechanism of polystyrene microplastics (MPs) and the potential inhibitory effect of green tea extracts on MPs intestinal absorption
Toxicol In Vitro. 2024 Mar 24;97:105813. doi: 10.1016/j.tiv.2024.105813. Online ahead of print.ABSTRACTThe aims of the current study included characterizing the intestinal transport mechanism of polystyrene microplastics (MPs) with different charges and sizes in the intestinal epithelial cell model and determining the inhibitory effect of green tea extracts (GTEs) on the intestinal absorption of MPs in Caco-2 cells. The smaller sizes, which included diameters of 0.2 μm, of amine-modified MPs compared to either larger size (1 μm diameter, or carboxylate-MPs (0.2 and 1 μm diameter) significantly lowered the cell viability...
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Woo-Hyun Kim Dong-Ho Lee Jeong-Eun Kim Hyun Woo Jeong Jin-Oh Chung JongHwa Roh WanGi Kim Xiaoting Fu Soon-Mi Shim Source Type: research

In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 24;97:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays....
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research

Characterization of the intestinal transport mechanism of polystyrene microplastics (MPs) and the potential inhibitory effect of green tea extracts on MPs intestinal absorption
Toxicol In Vitro. 2024 Mar 22:105813. doi: 10.1016/j.tiv.2024.105813. Online ahead of print.ABSTRACTThe aims of the current study included characterizing the intestinal transport mechanism of polystyrene microplastics (MPs) with different charges and sizes in the intestinal epithelial cell model and determining the inhibitory effect of green tea extracts (GTEs) on the intestinal absorption of MPs in Caco-2 cells. The smaller sizes, which included diameters of 0.2 μm, of amine-modified MPs compared to either larger size (1 μm diameter, or carboxylate-MPs (0.2 and 1 μm diameter) significantly lowered the cell viability of...
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Woo-Hyun Kim Dong-Ho Lee Jeong-Eun Kim Xiaoting Fu Hyun Woo Jeong Jin-Oh Chung JongHwa Roh WanGi Kim Soon-Mi Shim Source Type: research

In vitro immunotoxicity effects of carbendazim were inhibited by n-acetylcysteine in microglial BV-2 cells
Toxicol In Vitro. 2024 Mar 22:105812. doi: 10.1016/j.tiv.2024.105812. Online ahead of print.ABSTRACTCarbendazim (CBZ) is a benzimidazole fungicide widely used worldwide in industrial, agricultural, and veterinary practices. Although, CBZ was found in all brain tissues causing serious neurotoxicity, its impact on brain immune cells remain scarcely understood. Our study investigated the in vitro effects of CBZ on activated microglial BV-2 cells. Lipopolysaccharide (LPS)-stimulated BV-2 cells were exposed to increasing concentrations of CBZ and cytokine release was measured by ELISA, and Cytometric Bead Array (CBA) assays. Mi...
Source: Toxicology in Vitro - March 24, 2024 Category: Toxicology Authors: Narjesse E L Mabrouk Maha Mastouri G érard Lizard Mahjoub Aouni Hedi Harizi Source Type: research

DMC triggers MDA-MB-231 cells apoptosis via inhibiting protective autophagy and PI3K/AKT/mTOR pathway by enhancing ROS level
Toxicol In Vitro. 2024 Mar 21:105809. doi: 10.1016/j.tiv.2024.105809. Online ahead of print.ABSTRACTDMC, a kind of compound derived from the dry flower buds of Cleistocalyx operculatus, has been shown to inhibit the growth of various cancer cells, but research on triple-negative breast cancer cells remains scarce. To explore this issue, MDA-MB-231 cells were selected, and the results showed that DMC has strong proliferation inhibit effects on this kind of cells. The inhibit rate of 30 μM DMC incubated for 24 h was 56.25%, and 40.6% cells were arrested under the G2/M phase. The levels of pro-apoptosis protein Bax and activ...
Source: Toxicology in Vitro - March 23, 2024 Category: Toxicology Authors: Yu Jiang Sunjie Xu Miaomiao Guo Zhi Lu Xing Wei Faliang An Xiujuan Xin Source Type: research

Suitability of human HepaRG cells and liver spheroids as in vitro model to investigate the bioactivation of the organothiophosphate pesticide parathion
Toxicol In Vitro. 2024 Mar 21:105811. doi: 10.1016/j.tiv.2024.105811. Online ahead of print.ABSTRACTOrganophosphorus compounds (OP) constitute a large group of chemicals including pesticides and nerve agents. Organothiophosphate pesticides require cytochrome P450-mediated oxidative desulphuration in the liver to form corresponding oxons, which are potent inhibitors of the enzyme acetylcholinesterase (AChE). Human HepaRG cells are a promising tool to study liver-specific functions and have been shown to maintain drug metabolizing enzymes. This research describes for the first time the in vitro metabolic activation of an org...
Source: Toxicology in Vitro - March 23, 2024 Category: Toxicology Authors: Gabriele Horn Franz Worek Source Type: research

DMC triggers MDA-MB-231 cells apoptosis via inhibiting protective autophagy and PI3K/AKT/mTOR pathway by enhancing ROS level
Toxicol In Vitro. 2024 Mar 21:105809. doi: 10.1016/j.tiv.2024.105809. Online ahead of print.ABSTRACTDMC, a kind of compound derived from the dry flower buds of Cleistocalyx operculatus, has been shown to inhibit the growth of various cancer cells, but research on triple-negative breast cancer cells remains scarce. To explore this issue, MDA-MB-231 cells were selected, and the results showed that DMC has strong proliferation inhibit effects on this kind of cells. The inhibit rate of 30 μM DMC incubated for 24 h was 56.25%, and 40.6% cells were arrested under the G2/M phase. The levels of pro-apoptosis protein Bax and activ...
Source: Toxicology in Vitro - March 23, 2024 Category: Toxicology Authors: Yu Jiang Sunjie Xu Miaomiao Guo Zhi Lu Xing Wei Faliang An Xiujuan Xin Source Type: research

Suitability of human HepaRG cells and liver spheroids as in vitro model to investigate the bioactivation of the organothiophosphate pesticide parathion
Toxicol In Vitro. 2024 Mar 21:105811. doi: 10.1016/j.tiv.2024.105811. Online ahead of print.ABSTRACTOrganophosphorus compounds (OP) constitute a large group of chemicals including pesticides and nerve agents. Organothiophosphate pesticides require cytochrome P450-mediated oxidative desulphuration in the liver to form corresponding oxons, which are potent inhibitors of the enzyme acetylcholinesterase (AChE). Human HepaRG cells are a promising tool to study liver-specific functions and have been shown to maintain drug metabolizing enzymes. This research describes for the first time the in vitro metabolic activation of an org...
Source: Toxicology in Vitro - March 23, 2024 Category: Toxicology Authors: Gabriele Horn Franz Worek Source Type: research

Perfluorooctanoic acid inhibits cell proliferation through mitochondrial damage
Toxicol In Vitro. 2024 Mar 19:105810. doi: 10.1016/j.tiv.2024.105810. Online ahead of print.ABSTRACTGrown evidence has shown that the liver and reproductive organs were the main target organs of perfluorooctanoic acid (PFOA). Herein, we studied a toxic mechanism of PFOA using HeLa Chang liver epithelial cells. When incubated with PFOA for 24 h or 48 h, cell proliferation was inhibited in a concentration- and time-dependent fashion, but interestingly, the feature of dead cells was not notable. Mitochondrial volume was increased with concentration and time, whereas the mitochondrial membrane potential and produced ATP amount...
Source: Toxicology in Vitro - March 21, 2024 Category: Toxicology Authors: Eun-Jung Park Kexin Li Min-Sung Kang Jae-Won Choi BoSung Baek Yu-Kyeong Yang Art E Cho Byoung-Seok Lee Source Type: research

Perfluorooctanoic acid inhibits cell proliferation through mitochondrial damage
Toxicol In Vitro. 2024 Mar 19:105810. doi: 10.1016/j.tiv.2024.105810. Online ahead of print.ABSTRACTGrown evidence has shown that the liver and reproductive organs were the main target organs of perfluorooctanoic acid (PFOA). Herein, we studied a toxic mechanism of PFOA using HeLa Chang liver epithelial cells. When incubated with PFOA for 24 h or 48 h, cell proliferation was inhibited in a concentration- and time-dependent fashion, but interestingly, the feature of dead cells was not notable. Mitochondrial volume was increased with concentration and time, whereas the mitochondrial membrane potential and produced ATP amount...
Source: Toxicology in Vitro - March 21, 2024 Category: Toxicology Authors: Eun-Jung Park Kexin Li Min-Sung Kang Jae-Won Choi BoSung Baek Yu-Kyeong Yang Art E Cho Byoung-Seok Lee Source Type: research

Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves
Toxicol In Vitro. 2024 Mar 12;97:105808. doi: 10.1016/j.tiv.2024.105808. Online ahead of print.ABSTRACTThe use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure scenarios were performed aiming at: i) comparing the cellular response of two primary culture of keratinocytes (HEK and...
Source: Toxicology in Vitro - March 14, 2024 Category: Toxicology Authors: Catherine Martin Bertrand Evrard Fr édéric Percevault Kate Ryder Thomas Darde Aur élie Lardenois Maxim Zhadobov Ronan Sauleau Fr édéric Chalmel Yves Le Dr éan Denis Habauzit Source Type: research

Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves
Toxicol In Vitro. 2024 Mar 12;97:105808. doi: 10.1016/j.tiv.2024.105808. Online ahead of print.ABSTRACTThe use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure scenarios were performed aiming at: i) comparing the cellular response of two primary culture of keratinocytes (HEK and...
Source: Toxicology in Vitro - March 14, 2024 Category: Toxicology Authors: Catherine Martin Bertrand Evrard Fr édéric Percevault Kate Ryder Thomas Darde Aur élie Lardenois Maxim Zhadobov Ronan Sauleau Fr édéric Chalmel Yves Le Dr éan Denis Habauzit Source Type: research

Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves
Toxicol In Vitro. 2024 Mar 12;97:105808. doi: 10.1016/j.tiv.2024.105808. Online ahead of print.ABSTRACTThe use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure scenarios were performed aiming at: i) comparing the cellular response of two primary culture of keratinocytes (HEK and...
Source: Toxicology in Vitro - March 14, 2024 Category: Toxicology Authors: Catherine Martin Bertrand Evrard Fr édéric Percevault Kate Ryder Thomas Darde Aur élie Lardenois Maxim Zhadobov Ronan Sauleau Fr édéric Chalmel Yves Le Dr éan Denis Habauzit Source Type: research