Naja nigricincta nigricincta venom, a murine model. Evaluation of skeletal and cardio-myonecrosis, kidney injury and inflammatory response along with neutralisation efficacy by the SAIMR/SAVP - and EchiTAb-Plus-ICP polyvalent antivenoms
Toxicon. 2024 Apr 15:107719. doi: 10.1016/j.toxicon.2024.107719. Online ahead of print.ABSTRACTAfrican spitting cobra, Naja nigricincta nigricincta (Zebra snake), envenomation is an important cause of snakebite morbidity and mortality in Namibia. The snake is endemic to central and northern Namibia as well as southern Angola. The venom is mainly cytotoxic, resulting in aggressive dermo-necrosis and often accompanied by severe systemic complications. No specific antivenom exists. Rhabdomyolysis, systemic inflammatory response, haemostatic abnormalities, infective necrotising fasciitis as well as acute kidney failure have be...
Source: Toxicon - April 17, 2024 Category: Toxicology Authors: Esta L Saaiman Engelbrecht Vinny Naidoo Christo J Botha Source Type: research
Efficacy of Maturase K and rpL20 protein extracted from C. procera leaves on Anopheles stephensi
Toxicon. 2024 Apr 14:107714. doi: 10.1016/j.toxicon.2024.107714. Online ahead of print.ABSTRACTThe present work is carried out to protein isolation, purification, and characterization from leaves, stem, and seed of C. procera and to evaluate the larvicidal potential on Anopheles stephensi. The whole protein was isolated using protein extraction buffer and precipitated by ammonium sulphate and larvicidal active protein was purified by the column chromatography. The homogeneity of larvicidal protein was confirmed by the SDS-PAGE. The identification of protein was done by the HPLC and LC-MS/ESI-MS. The crude protein from leav...
Source: Toxicon - April 16, 2024 Category: Toxicology Authors: Manisha Kirar S P Singh Hitesh Singh Neelam Sehrawat Source Type: research
Efficacy of Maturase K and rpL20 protein extracted from C. procera leaves on Anopheles stephensi
Toxicon. 2024 Apr 14:107714. doi: 10.1016/j.toxicon.2024.107714. Online ahead of print.ABSTRACTThe present work is carried out to protein isolation, purification, and characterization from leaves, stem, and seed of C. procera and to evaluate the larvicidal potential on Anopheles stephensi. The whole protein was isolated using protein extraction buffer and precipitated by ammonium sulphate and larvicidal active protein was purified by the column chromatography. The homogeneity of larvicidal protein was confirmed by the SDS-PAGE. The identification of protein was done by the HPLC and LC-MS/ESI-MS. The crude protein from leav...
Source: Toxicon - April 16, 2024 Category: Toxicology Authors: Manisha Kirar S P Singh Hitesh Singh Neelam Sehrawat Source Type: research
Using network pharmacology and molecular docking to uncover the mechanism by which quercetin alleviates deoxynivalenol-induced porcine intestinal injury
Toxicon. 2024 Apr 12:107709. doi: 10.1016/j.toxicon.2024.107709. Online ahead of print.ABSTRACTDeoxynivalenol is a widespread feed contaminant that leads to vomit, which results in serious symptom such as increased intestinal permeability and even intestinal mucosal necrosis. Recent studies have reported the role of quercetin in alleviating deoxynivalenol-induced intestinal injury; however, the mechanisms and targets remain unclear. Thus, we aimed to identify the mechanisms of action by using a combination of network pharmacology and molecular docking. We identified 151 quercetin targets, 235 deoxynivalenol targets and 47 ...
Source: Toxicon - April 14, 2024 Category: Toxicology Authors: Pu Guo Xuemin Li Yunda Xue Qirong Lu Yu Liu Jianglin Xiong Zhongyuan Wu Shulin Fu Chun Ye Xu Wang Yinsheng Qiu Source Type: research
Interaction of beauvericin in combination with antibiotics against methicillin-resistant Staphylococcus aureus and Salmonella typhimurium
Toxicon. 2024 Apr 12:107713. doi: 10.1016/j.toxicon.2024.107713. Online ahead of print.ABSTRACTMultidrug resistance in bacteria is a major challenge worldwide, increasing both mortality by infections and costs for the health systems. Therefore, it is of utmost importance to find new drugs against resistant bacteria. Beauvericin (BEA) is a mycotoxin produced by entomopathogenic and other fungi of the genus Fusarium. Our work determines the effect of BEA combined with antibiotics, which has not been previously explored. The combination analysis included different antibiotics against non-methicillin-resistant Staphylococcus a...
Source: Toxicon - April 14, 2024 Category: Toxicology Authors: V ásquez Bonilla J Norberto Barranco Florido J Esteban A Hamdan Partida Ponce Alquicira Edith Octavio Loera Source Type: research
Pine needle abortions in cattle due to consumption of Pinus ponderosa in Argentina: Case reports
Toxicon. 2024 Apr 11;242:107712. doi: 10.1016/j.toxicon.2024.107712. Online ahead of print.ABSTRACTTwo outbreaks of pine needle abortions in cattle are here reported for the first time in Argentina. The cases occurred in Chubut and Neuquén provinces in the Patagonia region, causing 29.6% and 9% of abortions in each herd respectively. In both outbreaks, the dams were in the last third of gestation, and, due to a period of cold, snow and lack of available forage, they gained access to Pinus ponderosa and Pinus contorta forests. No pathological lesions, serological, molecular, or microbiological evidence of infectious causes...
Source: Toxicon - April 13, 2024 Category: Toxicology Authors: A Martinez R Apostolo R Herrera D Gardner L Borrelli G Zabaleta C Robles J Ferrer ía Source Type: research
Synergistic effects of Metformin and Forskolin on oxidative stress induced by diabetes and hepatocellular cancer: an animal study
CONCLUSION: Metformin and Forskolin reduced oxidative stress in diabetic and HCC-induced rats. The results indicated that the combination of agents (Metformin & Forskolin) had greater therapeutic effects than Forskolin alone in reducing glucose levels in diabetic rats. However, the ameliorative effects of combining Metformin and Forskolin on blood and urine oxidative stress were not statistically higher than those of Metformin alone.PMID:38614244 | DOI:10.1016/j.toxicon.2024.107720 (Source: Toxicon)
Source: Toxicon - April 13, 2024 Category: Toxicology Authors: Qian Yang Mohammed Abed Jawad Abdullah Ali Alzahrani Zahraa F Hassan Ahmed Elawady Ahmed Hjazi Mehran Naghibi Source Type: research
Intravenous Administration of Recombinant Ph α1β: Antinociceptive Properties and Morphine Tolerance Reversal in a Cancer-Associated Pain Model
This study aims to assess the antinociceptive effects resulting from intravenous administration of the recombinant form of Phα1β (r-Phα1β) in an experimental model of cancer-related pain in mice, tolerant or not to morphine. The model of cancer-induced pain was used to evaluate these effects, with the injection of B16F10 cells, followed by the administration of the r-Phα1β, and evaluation of the mechanical threshold by the von Frey test. Also, adverse effects were assessed using a score scale, the rotarod, and open field tests. Results indicate that the administration of r-Phα1β provoked antinociception in animals ...
Source: Toxicon - April 13, 2024 Category: Toxicology Authors: Mariana de Melo Cardoso Rahisa Scussel Jessica da Silva Abel Fernando Oriques Pereira Lidiane Anast ácio Cruz Franciane da Costa Constante Ellen De Pieri Helena Mendes Abelaira Juliano Ferreira Marcus Vin ícius Gomez Fl ávia K Rigo Ricardo Andrez Macha Source Type: research
Individual cytotoxicity of three major type A trichothene, T-2, HT-2, and diacetoxyscirpenol in human Jurkat T cells
Toxicon. 2024 Apr 11:107718. doi: 10.1016/j.toxicon.2024.107718. Online ahead of print.ABSTRACTMycotoxins are toxic, fungal secondary metabolites that contaminate agricultural commodities, food, and feed. Among them, T-2, HT-2, and diacetoxyscirpenol (DAS; the major type A trichothecene) are primarily produced from Fusarium species. These mycotoxins exert numerous toxicological effects in animals and humans, such as dermatotoxicity, haematotoxicity, hepatotoxicity, nephrotoxicity, neurotoxicity, and immunotoxicity. In the present study, human Jurkat T cells were used as a model to investigate apoptotic cell death induced b...
Source: Toxicon - April 13, 2024 Category: Toxicology Authors: Phattarawadee Wattanasuntorn Patchara Phuktes Saranya Poapolathep Sontana Mimapan Muncharee Tattiyapong Johanna Fink-Gremmels Isabelle P Oswald Amnart Poapolathep Source Type: research
ROLE OF NITRIC OXIDE AND SIGNALING PATHWAYS MODULATING THE STIMULATORY EFFECT OF SNAKE VENOM SECRETORY PLA < sub > 2 < /sub > S ON NON-OPSONIZED ZYMOSAN PHAGOCYTOSIS BY MACROPHAGES
Toxicon. 2024 Apr 11:107716. doi: 10.1016/j.toxicon.2024.107716. Online ahead of print.ABSTRACTThe phagocytic activity of macrophages activated with MT-II, a Lys-49 PLA2 homolog, and MT-III, an Asp-49 PLA2, from Bothrops asper snake venom, was investigated in this study using a pharmacological approach. Stimulating thioglycollate-elicited macrophages with both venom components enhanced their ability to phagocytose non-opsonized zymosan particles. MT-II and MT-III-induced phagocytosis was drastically inhibited by pretreating cells with L-NAME, aminoguanidine or L-NIL, cNOS or iNOS inhibitors, or with ODQ (sGC inhibitor) or ...
Source: Toxicon - April 13, 2024 Category: Toxicology Authors: Juliana Pavan Zuliani Jos é María Gutiérrez Catarina Teixeira Source Type: research
The PLA < sub > 2 < /sub > inhibitor from Crotalus durissus terrificus blood plasma (CNF) inhibits group III-PLA < sub > 2 < /sub > from honeybee venom
Toxicon. 2024 Apr 5;242:107711. doi: 10.1016/j.toxicon.2024.107711. Online ahead of print.ABSTRACTCrotalus neutralizing factor (CNF) is an endogenous glycoprotein from Crotalus durissus terrificus snake blood that inhibits secretory phospholipases A2 (sPLA2) from the Viperid but not from Elapid venoms (subgroups IA and IIA, respectively). In the present study, we demonstrated that CNF can inhibit group III-PLA2 from bee venom by forming a stable enzyme-inhibitor complex. This finding opens up new possibilities for the potential use of CNF and/or CNF-based derivatives in the therapeutics of bee stings.PMID:38583578 | DOI:10...
Source: Toxicon - April 7, 2024 Category: Toxicology Authors: Paula Ladeira Ortolani Patr ícia Cota Campos Consuelo Latorre Fortes-Dias Source Type: research
The PLA < sub > 2 < /sub > inhibitor from Crotalus durissus terrificus blood plasma (CNF) inhibits group III-PLA < sub > 2 < /sub > from honeybee venom
Toxicon. 2024 Apr 5;242:107711. doi: 10.1016/j.toxicon.2024.107711. Online ahead of print.ABSTRACTCrotalus neutralizing factor (CNF) is an endogenous glycoprotein from Crotalus durissus terrificus snake blood that inhibits secretory phospholipases A2 (sPLA2) from the Viperid but not from Elapid venoms (subgroups IA and IIA, respectively). In the present study, we demonstrated that CNF can inhibit group III-PLA2 from bee venom by forming a stable enzyme-inhibitor complex. This finding opens up new possibilities for the potential use of CNF and/or CNF-based derivatives in the therapeutics of bee stings.PMID:38583578 | DOI:10...
Source: Toxicon - April 7, 2024 Category: Toxicology Authors: Paula Ladeira Ortolani Patr ícia Cota Campos Consuelo Latorre Fortes-Dias Source Type: research
The PLA < sub > 2 < /sub > inhibitor from Crotalus durissus terrificus blood plasma (CNF) inhibits group III-PLA < sub > 2 < /sub > from honeybee venom
Toxicon. 2024 Apr 5;242:107711. doi: 10.1016/j.toxicon.2024.107711. Online ahead of print.ABSTRACTCrotalus neutralizing factor (CNF) is an endogenous glycoprotein from Crotalus durissus terrificus snake blood that inhibits secretory phospholipases A2 (sPLA2) from the Viperid but not from Elapid venoms (subgroups IA and IIA, respectively). In the present study, we demonstrated that CNF can inhibit group III-PLA2 from bee venom by forming a stable enzyme-inhibitor complex. This finding opens up new possibilities for the potential use of CNF and/or CNF-based derivatives in the therapeutics of bee stings.PMID:38583578 | DOI:10...
Source: Toxicon - April 7, 2024 Category: Toxicology Authors: Paula Ladeira Ortolani Patr ícia Cota Campos Consuelo Latorre Fortes-Dias Source Type: research
The PLA < sub > 2 < /sub > inhibitor from Crotalus durissus terrificus blood plasma (CNF) inhibits group III-PLA < sub > 2 < /sub > from honeybee venom
Toxicon. 2024 Apr 5:107711. doi: 10.1016/j.toxicon.2024.107711. Online ahead of print.ABSTRACTCrotalus neutralizing factor (CNF) is an endogenous glycoprotein from Crotalus durissus terrificus snake blood that inhibits secretory phospholipases A2 (sPLA2) from the Viperid but not from Elapid venoms (subgroups IA and IIA, respectively). In the present study, we demonstrated that CNF can inhibit group III-PLA2 from bee venom by forming a stable enzyme-inhibitor complex. This finding opens up new possibilities for the potential use of CNF and/or CNF-based derivatives in the therapeutics of bee stings.PMID:38583578 | DOI:10.101...
Source: Toxicon - April 7, 2024 Category: Toxicology Authors: Paula Ladeira Ortolani Patr ícia Cota Campos Consuelo Latorre Fortes-Dias Source Type: research
The PLA < sub > 2 < /sub > inhibitor from Crotalus durissus terrificus blood plasma (CNF) inhibits group III-PLA < sub > 2 < /sub > from honeybee venom
Toxicon. 2024 Apr 5:107711. doi: 10.1016/j.toxicon.2024.107711. Online ahead of print.ABSTRACTCrotalus neutralizing factor (CNF) is an endogenous glycoprotein from Crotalus durissus terrificus snake blood that inhibits secretory phospholipases A2 (sPLA2) from the Viperid but not from Elapid venoms (subgroups IA and IIA, respectively). In the present study, we demonstrated that CNF can inhibit group III-PLA2 from bee venom by forming a stable enzyme-inhibitor complex. This finding opens up new possibilities for the potential use of CNF and/or CNF-based derivatives in the therapeutics of bee stings.PMID:38583578 | DOI:10.101...
Source: Toxicon - April 7, 2024 Category: Toxicology Authors: Paula Ladeira Ortolani Patr ícia Cota Campos Consuelo Latorre Fortes-Dias Source Type: research
