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SEM Study of apical morphological alterations in primary teeth with vital and necrotic pulps.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, there are morphological differences in the apical region of primary teeth with different pulpal and periapical pathologies. PMID: 19895290 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: da Silva LA, Fernandes PM, da Silva RA, Nelson-Filho P, Rocha LB, Rossi MA Tags: Ultrastruct Pathol Source Type: journals

Examination of native and carbamide peroxide-bleached human tooth enamel by atomic force microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Atomic force microscopy (AFM) was used to study the effects of bleaching on the morphology of the enamel surface with nanoscale resolution. Samples of human tooth enamel with native (pumiced) or fine-polished surfaces were examined before and after bleaching with 30% carbamide peroxide. The obtained profilometric AFM data revealed significant morphological surface alterations. After 1 h of bleaching, the surface roughness increased significantly from 19 +/- 4nm to 33 +/- 5 nm. Six-hour bleaching did not produce any significant further increase in enamel surface roughness. The interrod junction depth raised more than tw...
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: Mahringer C, Fureder M, Kastner M, Ebner A, Hinterdorfer P, Vitkov L, Hannig M, Kienberger F, Schilcher K Tags: Ultrastruct Pathol Source Type: journals

Chromatin ultrastructural abnormalities in leukocytes, as peripheral markers of bipolar patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated the ultrastructural conformation changes of the chromatin in blood leukocytes of bipolar patients, versus normal controls, by using the phosphotungstic acid-hematoxylin (PTAH) block-staining method, modified for electron microscopy, and the immunohistochemical localization of the histone H1, by the immunogold method. These two methods are basically complementary. If histone H1 immunolabeling is used, it shows that the immunogold labeling on chromatin is different in the three phases of the illness, i.e., high in normothymia and low in depression as well as in mania. However, in this particular tissu...
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: Chrysanthou-Piterou M, Havaki S, Alevizos V, Papadimitriou GN, Issidorides MR Tags: Ultrastruct Pathol Source Type: journals

Effect of dobutamine and hyperoxia on free radicals production in relation to the ultrastructural alterations in the endothelial of myocardial capillary in rats, Rattus norvigicus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hyperoxia has been widely used as model for oxidative stress. Free radicals (FR), suproxide anion (*O(-)(2)) and nitric oxide anion (*NO(-)), are highly toxic and have detrimental effects on nitroso-redox balance in the myocyte. Myocardium is rich with beta-adrenergic receptors and endothelial is the site of NO production. The authors hypothesized that graded doses of dobutamine result in hyperkinetic state, which shifts the nitroso-redox balance toward the buildup of reactive species in dose-dependent excess. The purpose of the present study was to investigate free radicals production and coronary endothelial cell pat...
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: Mattar EH, Haffor AS Tags: Ultrastruct Pathol Source Type: journals

Macrothrombocytopenia: investigating the ultrastructure of platelets and fibrin networks using scanning and transmission electron microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Macrothrombocytopenia is a rare condition where large, circulating platelets ranging between approximately 5 and 20 microm are found (typically platelets size range from 1.5 to 2.5 microm). The condition is also characterized by the prevalence of decreased numbers of circulating platelets, bleeding, short circulating times in blood, as well as abnormal platelet destruction. The current research investigates the scanning electron microscopy (SEM) and transmission electron microscopy (TEM) of platelet aggregates and fibrin networks of a family diagnosed with macrothrombocytopenia. Although TEM analysis of macrothrombocyt...
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: Pretorius E, Oberholzer HM, van der Spuy WJ, Meiring JH Tags: Ultrastruct Pathol Source Type: journals

Viral Haufen are urinary biomarkers of polyomavirus nephropathy: New diagnostic strategies utilizing negative staining electron microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Haufen, i.e. discrete three-dimensional cast-like polyomavirus aggregates in the urine, mark polyoma BK-virus nephropathy (BKN) with positive and negative predictive values of greater than 95%. They are novel diagnostic biomarkers of BKN, an important infectious complication post kidney transplantation. Here, we describe technical details of Haufen detection by negative staining electron microscopy. We studied more than 400 urine samples from over 180 patients and developed easy-to-follow protocols for optimal specimen preservation and preparation, including sample clarification and concentration. We detail diagnostic ...
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: Singh HK, Donna Thompson B, Nickeleit V Tags: Ultrastruct Pathol Source Type: journals

Invasion of erythroblasts by Pasmodium vivax: A new mechanism contributing to malarial anemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Severe malarial anemia causes considerable mortality and morbidity in endemic areas. Possible mechanisms underlying the anemia include lysis of parasitized and nonparasitized red cells as well as parasite product-mediated effects on erythropoiesis. The latter include suppression of erythropoiesis, dyserythropoiesis, and ineffective erythropoiesis. Present transmission electron microscope data in two cases of Pasmodium vivax malaria show a hitherto undescribed mechanism contributing to malarial anemia, namely, infection of erythroblasts by parasites and their subsequent degradation. No parasites were detected in the per...
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: Ru YX, Mao BY, Zhang FK, Pang TX, Zhao SX, Liu JH, Wickramasinghe SN Tags: Ultrastruct Pathol Source Type: journals

Ribosome-lamella complexes in injured podocytes: description of a case and review of the literature.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ribosome-lamella complexes (RLCs) are mainly observed in a variety of hematological disorders and occasionally in solid neoplasms and in nonneoplastic diseases. These intracytoplasmic organelles are held to arise from rough endoplasmic reticulum, but, in agreement with more recent literature data, their function is still unclear. Ultrastructural analysis of glomeruli from a patient with focal segmental glomerulosclerosis secondary to metabolic syndrome disclosed significant foot process loss and abundant cytoskeletal proteins in major podocyte processes; two of the latter also displayed RLCs. This is the second report ...
Source: Ultrastructural Pathology - October 1, 2009 Category: Pathology Authors: Cangiotti AM, Morroni M Tags: Ultrastruct Pathol Source Type: journals

Distribution of molecular breast cancer subtypes in middle eastern-saudi arabian women: a pilot study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study uses gene expression profiling by quantitative real time polymerase chain reaction (qRT-PCR) to identify "intrinsic" subtypes in a Saudi population of breast cancers and compares the distribution of subtypes to the more commonly profiled Caucasian population. In addition, the immunohistochemical profile of breast cancers was correlated to the gene expression analysis. Discrepancy rate of 39% in subtype prediction between gene expression and immunohistochemical profile of the tumors was noticed. Most of this variation was in the luminal subtype. Frequency of HER2+ subtype in the Saudi cases was high (28%) by both...
Source: Ultrastructural Pathology - June 30, 2009 Category: Pathology Authors: Al-Tamimi DM, Bernard PS, Shawarby MA, Al-Amri AM, Hadi MA Tags: Ultrastruct Pathol Source Type: journals

Bartonellosis as cause of death after red blood cell unit transfusion.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The authors present the case of a young man with aplastic anemia who went into shock and died after several red blood cell unit transfusions. Immunohematological studies did not show any abnormality and blood cultures from patients and blood bags were negative. The ultrastructural findings, allied with current scientific knowledge, permitted the diagnosis of Bartonella sp. infection. In face of this diagnosis, two possibilities should be considered: the first one is that the patient was already infected by the bacteria before the last RBC unit transfusion. The pathogen could be involved in aplastic anemia etiology and ...
Source: Ultrastructural Pathology - June 30, 2009 Category: Pathology Authors: Magalhães RF, Urso Pitassi LH, Lania BG, Barjas-Castro ML, Neves Ferreira Velho PE Tags: Ultrastruct Pathol Source Type: journals

Oral pulse granuloma: histological findings by confocal laser scanning microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, this study reports the fine definition of pulse granuloma at microscopic level by CLSM and the results warrant the use of this technique for further analyses. PMID: 19728231 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - June 30, 2009 Category: Pathology Authors: Scivetti M, Lucchese A, Ficarra G, Giuliani M, Lajolo C, Maiorano E, Favia G Tags: Ultrastruct Pathol Source Type: journals

Differentiation effect of pyruvate and uridine on cultured U937-rho degrees cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The human pro-monocytic leukemia U937 cell line was previously reported to become rho degrees cells after a long-term ethidium bromide exposure. In the authors' extensive PCR studies with different pairs of primers for the mtDNA molecule they showed that these U937-rho degrees cells, after being cultured in their laboratory for a time, did replete their mtDNA. That the cells grew well in the normal medium (RPMI 1640 plus 10% fetal calf serum and 2 mM l-glutamine) as the parental cells also suggests that these cells contain functional mitochondria and mtDNA molecules. Further experiments showed that the cells cultured i...
Source: Ultrastructural Pathology - June 30, 2009 Category: Pathology Authors: Liu Y, Geng L, Suo Z Tags: Ultrastruct Pathol Source Type: journals

Perineurioma of the adrenal gland.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The authors report the first case of perineurioma of the adrenal gland. The tumor was composed of elongated wavy spindle cells focally arranged in a fascicular pattern. It was positive for epithelial membrane antigen (EMA) and claudin-1, and was negative for S-100 protein and glial fibrillary acidic protein (GFAP). Electron microscopy showed long, slender cytoplasmic processes coated by discontinuos basal lamina and presence of many pinocytotic vesicles. PMID: 19728233 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - June 30, 2009 Category: Pathology Authors: Rampisela D, Donner LR Tags: Ultrastruct Pathol Source Type: journals

A shorter fixation protocol for transmission electron microscopy: an alternative to spend less time.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The performance of a moderately shorter fixation protocol for transmission electron microscopy (TEM) was evaluated by analyzing the cell structure quality after the processing. The relevance of this experimental technique is mainly based on reducting time of the steps of conventional protocols: fixation, washes, dehydration, and epoxy resin infiltration. Two sources of murine cells were used, the peritoneal and mesenteric lymph node cells. A fixation and material processing faster than usual methods can save time and improve results. Samples analysis indicated good preservation of different cell structures and organell...
Source: Ultrastructural Pathology - June 30, 2009 Category: Pathology Authors: Guimaraes Fde S, de Oliveira SM, de Oliveira CC, Donatti L, Buchi Dde F Tags: Ultrastruct Pathol Source Type: journals

Identification of cancer stem-like side population cells in ovarian cancer cell line OVCAR-3.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the authors aimed to disclose whether the hoechst33342 staining required extensive optimization for identifying SP cells in the human ovarian cancer cell line OVCAR-3. After systematic evaluations, it was found that only 2.5 microg/mL hoechst33342 staining of the cells for 60 min could get an ideal SP population, which accounted for 0.9% of the whole cell population. The sorted SP cells showed significantly higher colony formation efficiency than the non-side population (NSP) cells, and only the SP cells could form holoclones. Real-time PCR disclosed that SP cells expressed higher levels of "stemness" gene O...
Source: Ultrastructural Pathology - June 30, 2009 Category: Pathology Authors: Gao Q, Geng L, Kvalheim G, Gaudernack G, Suo Z Tags: Ultrastruct Pathol Source Type: journals

Qualitative electron microscopic analysis of cultured chick embryonic cardiac and skeletal muscle cells: the cellular effect of coenzyme q10 after exposure to triton x-100.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The numerous protective effects of coenzyme Q10 (CoQ10) evoked the question of whether it might be able to elicit protection to cell membranes after being challenged by the membrane disrupter Triton X-100. Cardiac and skeletal muscle tissue from chick embryos was cultured and exposed to increasing concentrations of CoQ10 and Triton X-100. Scanning electron microscopy was used to study cell morphology. Results suggested the ability of CoQ10 to offer protection to cells challenged by Triton X-100. The authors suggest that CoQ10 may offer protection to muscle cells, by enhancing membrane repair via patch formation by an u...
Source: Ultrastructural Pathology - June 27, 2009 Category: Pathology Authors: Potgieter M, Pretorius E, Oberholzer HM Tags: Ultrastruct Pathol Source Type: journals

The extracellular space in the edematous human cerebral cortex: an electron microscopic study using cortical biopsies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In a vascular anomaly showing moderate edema, the extracellular space appeared apparently normal, exhibiting a membrane to membrane space of about 20 nm in width. In congenital hydrocephalus, this space appeared notably enlarged and occupied by an electron transparent, nonproteinaceous interstitial edema fluid, due to abnormal accumulation of cerebrospinal fluid. In brain trauma, the distended extracellular space contained either electron-lucid nonproteinaceous or electron-dense proteinaceous edema fluid. Hemorrhagic foci, fibrinoid material, and non-nervous invading cells, such as macrophages and monocytes, were also ...
Source: Ultrastructural Pathology - June 27, 2009 Category: Pathology Authors: Castejon OJ Tags: Ultrastruct Pathol Source Type: journals

Effect of hyperoxia on the ultrastructural pathology of alveolar epithelium in relation to glutathione peroxidase, lactate dehydrogenase activities, and free radical production in rats, Rattus norvigicus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hyperoxia (HP) exposure inducts reactive oxygen species (ROS) in the lungs that may result in lung injury, including alveolar epithelial and endothelial cells. Lactate dehydrogenase (LDH) activity relates to glycolysis, whereas glutathione peroxidase (Gpx) activity relies on the pentose phosphate pathway (PPP). The purpose of this study was to examine early ROS-induced alveolar pathological changes in relation to the activity of glutathione peroxidase (GPx) and lactate dehydrogenase (LDH) activity. Twenty adult male rats, matched with age and body weight, were randomly assigned to two groups, control and experimental. ...
Source: Ultrastructural Pathology - June 27, 2009 Category: Pathology Authors: Bin-Jaliah I, Dallak M, Haffor AS Tags: Ultrastruct Pathol Source Type: journals

Ultrastructural analysis of amyloidoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Amyloidomas are localized mass-forming deposits of amyloid that occur with or without association with systemic amyloidosis. The ultrastructural findings in 3 amyloidomas from 2 autopsy patients with primary systemic AL amyloidosis are described. By transmission electron microscopy, there were randomly oriented nonbranching fibrils showing some unusual curvilinear forms and considerable variability in fibril diameter (two subsets of fibrils, one 12-14 nm and another 28-30 nm in diameter). The larger fibrils showed features of microtubule formation. Scanning electron microscopy demonstrated complex 3-dimensional tangles...
Source: Ultrastructural Pathology - June 27, 2009 Category: Pathology Authors: Garcia CA, Abell-Aleff PC, Gamb SI, Miller DV Tags: Ultrastruct Pathol Source Type: journals

Effects of Norway spruce (Picea abies) resin on cell wall and cell membrane of Staphylococcus aureus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Resin salve prepared from Norway spruce (Picea abies) has been used for centuries in traditional medicine to treat skin diseases. The authors studied with transmission and scanning electron microscopy, and with electron physiology, changes in cell wall and cell membrane of Staphylococcus aureus after exposure of the bacterial cultures to resin. After exposure, cell wall thickening, cell aggregation, changed branching of fatty acids, and dissipation of membrane potential of the bacterial cells were observed. The authors conclude that spruce resin affects the cell viability via changes in the cell wall and membrane, and ...
Source: Ultrastructural Pathology - June 27, 2009 Category: Pathology Authors: Sipponen A, Peltola R, Jokinen JJ, Laitinen K, Lohi J, Rautio M, Mannisto M, Sipponen P, Lounatmaa K Tags: Ultrastruct Pathol Source Type: journals

Myofibroblast transformation in metastatic extramedullary chronic myeloid leukemia: a case report.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Primary and metastatic carcinomas have a reactive stroma characterized by many myofibroblasts. These cells have also been documented in nonepithelial malignancies, such as sarcomas, malignant melanoma, and lymphoid tumors but in generally far fewer numbers. In non-Hodgkin's lymphoma, Hodgkin's disease, and leukemia, myofibroblasts are rather rarely documented. In particular, there appear to be no reports of myofibroblasts in either primary bone-marrow/peripheral blood leukemia or secondary deposits of leukemia. In this paper, a case of a relapsed chronic myeloid leukemia appearing in an inguinal lymph node is described...
Source: Ultrastructural Pathology - June 27, 2009 Category: Pathology Authors: Ru YX, Eyden B, Li QH, Mao BY, Liu EB, Pang TX Tags: Ultrastruct Pathol Source Type: journals

Effects of cadmium exposure on the ultrastructural pathology of different pulmonary cells, leukocyte count, and activity of glutathione peroxidase and lactate dehydrogenase in relation to free radical production in Uromastyx aegyptius.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Animal studies on the toxicity of heavy metals have been widely used as model to simulate the impacts of environmental pollution on the human health. In the present study the authors hypothesized that cadmium exposure inducts changes in reactive oxygen species (ROS) and that may be involved in the pathogenesis of lung diseases. The pathological changes of different pulmonary cells of ROS-cadmium-dependent effects were investigated in relation to the activity of lactate dehydrogenase (LDH) and glutathione peroxidase (GPx). Twelve animals were randomly assigned to two groups, control and experimental. The experimental gr...
Source: Ultrastructural Pathology - March 1, 2009 Category: Pathology Authors: Al-Johany AM, Haffor AS Tags: Ultrastruct Pathol Source Type: journals

Confocal laser scanning microscopy of liesegang rings in odontogenic cysts: analysis of three-dimensional image reconstruction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Liesegang rings are concentric noncellular lamellar structures, occasionally found in inflammatory tissues. They have been confused with various parasites, algas, calcification, and psammoma bodies. The authors examined Liesegang rings from oral inflammatory cysts by both optical and confocal laser scanning microscopy, and perfomed a three-dimensional reconstruction. These investigations indicate that Liesegang rings are composed of multiple birefringent concentric rings, resulting from a progressive deposition of organic substances, with an unclear pathogenesis. PMID: 19274580 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - March 1, 2009 Category: Pathology Authors: Scivetti M, Lucchese A, Crincoli V, Pilolli GP, Favia G Tags: Ultrastruct Pathol Source Type: journals

Ultrastructural and immunohistochemical analysis of fibrous long-spacing collagen fibrils in malignant mesothelioma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Three cases of biphasic mesothelioma and 2 cases of sarcomatoid mesothelioma were investigated using light and electron microscopy. In 2 of the 3 cases of biphasic mesotheliomas, fibrous long-spacing (FLS) collagen fibrils were discovered with a symmetrical cross-striation of 130 nm in periodicity. However, no connection between the FLS fibrils and usual collagen fibrils were observed. Periodic acid silver methenamine stain revealed unstained bands with periods of 130 nm in FLS fibrils, whereas the usual collagen fibrils showed continuous positive staining. All 3 cases of biphasic mesotheliomas showed deposits of hyalu...
Source: Ultrastructural Pathology - March 1, 2009 Category: Pathology Authors: Kang D, Kunugi S, Masuda Y, Ishizaki M, Koizumi K, Fukuda Y Tags: Ultrastruct Pathol Source Type: journals

Epithelioid sarcoma: a case report with ultrastructural confirmation of myofibroblastic differentiation based on fibronexus junctions.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Epithelioid sarcoma is an uncommon but well-described malignancy, which is found predominantly in the soft tissues of the young and middle-aged, and which pursues an indolent to aggressive course. It shows a degree of both mesenchymal and epithelial differentiation. Myofibroblastic differentiation has been recorded in epithelioid sarcoma for some time, the evidence being based mainly on the presence of smooth-muscle-type myofilaments and, more recently, on alpha-smooth-muscle actin and muscle-specific actin immunostaining. Myofibroblastic differentiation based on the stricter criterion of the fibronectin fibril/fibrone...
Source: Ultrastructural Pathology - March 1, 2009 Category: Pathology Authors: Eyden B, Wang G, Yao L Tags: Ultrastruct Pathol Source Type: journals

Significance of transmission electron microscopy in subtyping of monocytic leukemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this paper is to produce an ultrastructural classification of acute monocytic leukemia (AML-M5) in relation to clinical behaviors. The ultrastructural characteristics of blasts of the monocytic series were analyzed in 72 M5 patients by transmission electron microscopy (TEM), in terms of their content of typical monoblasts, atypical monoblasts, atypical promonocytes, and typical promonocytes in bone-marrow aspirates. Four kinds of monocytic blasts were identified by cell size and shape, nuclear profile, nucleocytoplasmic ratio, heterochromatin content, nucleolus, granules, vesicles, and Golgi apparatus. The...
Source: Ultrastructural Pathology - March 1, 2009 Category: Pathology Authors: Ru YX, Mi YC, Liu JH, Wang HJ, Zhao SX, Cui W, Li CW, Li QH, Zhu XF, Xiao ZJ, Pang JX, Wang JX Tags: Ultrastruct Pathol Source Type: journals

The lift technique: forty years of experience at the department of pathology, Children's Hospital of Pittsburgh.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Despite the widespread utilization of immunohistochemical stains in pathologic practice, virus identification remains a challenge. In our institution, electron microscopy combined with the lift technique has been utilized for 40 years as a reliable diagnostic tool where a question of viral infection is raised by light microscopic observation and could not be otherwise confirmed. The combination of light microscopic and ultrastructural methods has allowed us to examine individual cells suspicious for harboring viral particles previously identified on a hematoxylin-eosin stained tissue section. In this review we describe...
Source: Ultrastructural Pathology - March 1, 2009 Category: Pathology Authors: Walpusk J, Galambos C Tags: Ultrastruct Pathol Source Type: journals

Two-dimensional electrophoretic analysis of nuclear matrix proteins in human colon adenocarcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the present study was to observe possible qualitative and quantitative expression differences between nuclear matrix proteins (NMPs) of human colon adenocarcinoma and their mirror biopsies, using the technique of two-dimensional gel electrophoresis, in order to identify the existence of specific NMP fingerprints for colon cancer. Colon tissues were examined ultrastructurally and NMPs were isolated biochemically, by serial extraction of lipids, soluble proteins, DNA, RNA, and intermediate filaments and were separated according to their isoelectric point (pI) and their molecular weight (MW) by high-resolution ...
Source: Ultrastructural Pathology - March 1, 2009 Category: Pathology Authors: Toumpanaki A, Baltatzis GE, Gaitanarou E, Seretis E, Toumpanakis C, Aroni K, Kittas C, Voloudakis-Baltatzis IE Tags: Ultrastruct Pathol Source Type: journals

Ultrastructural observations on inflammatory angiogenesis in gastric carcinomas with massive neutrophil infiltration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, 4 cases of human gastric carcinomas with massive neutrophil infiltration were studied by light and electron microscopy, focusing on the inflammatory angiogenesis in the tumor stroma. At light microscopy, the tumors were advanced gastric carcinomas in which various degrees of tubular differentiation were present. Under an electron microscope, pericytes exhibited two major differentiated states with distinct ultrastructural features: a contractile phenotype and a synthetic phenotype. The contractile phenotype was characterized by abundant microfilaments. Synthetic pericytes contained abundant rough endoplasmic...
Source: Ultrastructural Pathology - January 1, 2009 Category: Pathology Authors: Caruso RA, Bonanno A, Finocchiaro G, Cavaliere R, Gitto G, Plutino FM, Quattrocchi E, Cuppari MC, Fabiano V, Basile G Tags: Ultrastruct Pathol Source Type: journals

Ultrastructural features of lung fibroblast differentiation into myofibroblasts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fibroblast differentiation into myofibroblast in transforming growth factor-beta1-exposed human lung fibroblasts and the immunolocalizations of alpha-smooth muscle actin, fibronectin, tenascin-C, and osteopontin in exposed cells were studied by conventional transmission electron microscopy and immunoelectron microscopy. Ultrastructural features of myofibroblasts were detected after exposure, e.g., alpha-smooth muscle actin positive bundles in the cytoplasm of cells and extracellular fibronectin-containing structures on the surface of the cell forming fibronexus structure, osteopontin adjacent to rough endoplastic retic...
Source: Ultrastructural Pathology - January 1, 2009 Category: Pathology Authors: Kaarteenaho-Wiik R, Paakko P, Sormunen R Tags: Ultrastruct Pathol Source Type: journals

A structure resembling basal/external lamina on the surface of plasma cells, and a discussion on intercellular contacts between hemolymphoid cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Basal/external lamina is not found over plasma cells or other hemolymphoid cells, and the feature can have diagnostic value in distinguishing the neoplastic counterparts of such cells from epithelium, endothelium, mesothelium, and so on, which do have this feature. In this paper, a material ultrastructurally indistinguishable from basal or external lamina is reported on reactive plasma cells found in a fibrous pseudotumor, intralobular stroma of normal breast, tumor stroma of squamous cell carcinoma, and submucosa of normal human small intestine. It was focal, followed the contours of the cell-surface membrane, was lig...
Source: Ultrastructural Pathology - January 1, 2009 Category: Pathology Authors: Eyden B Tags: Ultrastruct Pathol Source Type: journals

HCV has transforming potential to retrovirus: an ultrastructure hypothesis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present work highlights intracellular viral morphogenesis and virus-host cell interactions in patients proved to be infected with HCV. The material of this study consisted of 28 liver biopsies taken from patients positive for serum HCV-RNA by polymerase chain reaction. Liver biopsies were processed for light and electron microscopic examination. Ultrastructural findings of this work supported a new hypothesis for the turnover of HCV to retrovirus and described the presumed involved mechanism. This novel perception offers important insights that can explain the vague mechanisms of HCV behavior in the infected hepato...
Source: Ultrastructural Pathology - January 1, 2009 Category: Pathology Authors: Mansy SS, Abdelfatah AS, Hassanein MH Tags: Ultrastruct Pathol Source Type: journals

Fibronexus junctions associated with in vivo human endothelium.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The fibronexus is recognized as a characteristic marker of the myofibroblast. However, it is not completely specific for this cell, having been seen in aortic smooth muscle (in attenuated form) and endothelium in experimental animals. This paper documents fibronexus junctions in human in vivo endothelium. Ultrastructural observations were made on the vasculature of a desmoplastic and focally neurotropic malignant melanoma. Cross-sectioned fibronectin fibrils were seen outside the stromal surface of the endothelial plasmalemma. Often, they were positioned directly opposite the actin-filament bundles in the peripheral cy...
Source: Ultrastructural Pathology - January 1, 2009 Category: Pathology Authors: Eyden B Tags: Ultrastruct Pathol Source Type: journals

Carcinoma versus cytokeratin-positive lymphoma: a case report emphasizing the diagnostic role of electron microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lymphoma diagnosis rarely needs electron microscopy (EM), but one area where it can be useful is in the distinction of cytokeratin-positive lymphoma from carcinoma. The authors describe such a case, where difficulties were encountered due to lack of antibody specificity, distinguishing reactive from tumoral cells, and suboptimal sampling for EM. The tumor was in a lymph node next to the right submandibular gland in a 69-year-old man. This was a malignant tumor, composed of sheets of monomorphic large round cells. Interpretation on the part of a team of pathologists who examined this tumor was divided. On histological s...
Source: Ultrastructural Pathology - January 1, 2009 Category: Pathology Authors: Eyden B, Chakrabarty B, Hatimy U Tags: Ultrastruct Pathol Source Type: journals

Incidence of thin basement membrane nephropathy in 990 consecutive renal biopsies examined with electron microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thin basement membrane nephropathy is one of the main causes of hematuria in both children and adults. It is often associated with a family history and its true incidence is unknown. Accurate diagnosis of thin basement membrane nephropathy relies on the presence of attenuated glomerular basement membranes, a finding that can be appreciated only by examination in the electron microscope. In Cyprus the department of electron microscopy has received 990 consecutive renal biopsies for diagnosis. The aim of this study is to define the incidence of thin basement membrane nephropathy in this population sample based on the res...
Source: Ultrastructural Pathology - November 1, 2008 Category: Pathology Authors: Zouvani I, Aristodemou S, Hadjisavvas A, Michael T, Vassiliou M, Patsias C, Pierides A, Ioannou K, Kyriacou K Tags: Ultrastruct Pathol Source Type: journals

New diagnostic and molecular characteristics of malignant mesothelioma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Malignant mesothelioma is a primary cancer of the serosal cavities, an anatomic site that is also frequently affected by metastatic disease, predominantly from primary carcinomas of the lung, breast, and ovary. Advances in immunohistochemistry have resulted in improved diagnostic sensitivity and specificity in the differential diagnosis between metastatic adenocarcinoma and malignant mesothelioma in both cytological and histological material. Recently, the author's group applied high throughput technology to the identification of new markers that may aid in differentiating malignant mesothelioma from ovarian and perito...
Source: Ultrastructural Pathology - November 1, 2008 Category: Pathology Authors: Davidson B Tags: Ultrastruct Pathol Source Type: journals

Ultrastructural studies of glioma stem cells/progenitor cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, their ultrastructural features showed that GSPCs were at the primary stage of differentiation, and could even partially reveal the underlying reasons for the malignant proliferation and differential inhibition of GSPCs. PMID: 19117265 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - November 1, 2008 Category: Pathology Authors: Zhao Y, Huang Q, Zhang T, Dong J, Wang A, Lan Q, Gu X, Qin Z Tags: Ultrastruct Pathol Source Type: journals

BACE2 is stored in secretory granules of mouse and rat pancreatic beta cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
BACE2 is a protease homologous to BACE1 protein, an enzyme involved in the amyloid formation of Alzheimer disease (AD). However, despite the high homology between these two proteins, the biological role of BACE2 is still controversial, even though a few studies have suggested a pathogenetic role in sporadic inclusion-body myositis and hereditary inclusion-body myopathy, which are characterized by vacuolization of muscular fibers with intracellular deposits of proteins similar to those found in the brain of AD patients. Although BACE2 has also been identified in the pancreas, its function remains unknown and its specifi...
Source: Ultrastructural Pathology - November 1, 2008 Category: Pathology Authors: Finzi G, Franzi F, Placidi C, Acquati F, Palumbo E, Russo A, Taramelli R, Sessa F, La Rosa S Tags: Ultrastruct Pathol Source Type: journals

Renal medullary carcinoma: ultrastructural studies may benefit diagnosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Renal medullary carcinoma is a recently described highly aggressive malignancy that in most instances exhibits a constellation of clinical and light microscopic features sufficiently distinctive to enable a quick and confident diagnosis. Presented are three examples where, because of unusual elements in the clinical presentation, electron microscopic examination proved beneficial in establishing the diagnosis. PMID: 19117267 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - November 1, 2008 Category: Pathology Authors: Wartchow EP, Trost BA, Tucker JA, Albano EA, Mierau GW Tags: Ultrastruct Pathol Source Type: journals

Crocidolite and mesothelioma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study reports changes in the frequency of detection of various asbestos fiber types between 1982 and 2005. Crocidolite is increasingly detected in U.S. mesothelioma patients. The percentage of crocidolite fibers detected in lung tissue has risen from 4 to 10%, and the percentage of cases in which crocidolite was detected increased from 19 to 37%. Meanwhile, the frequency of detection of amosite and chrysotile has decreased. The authors performed a detailed analysis of cases in which crocidolite was identified in the absence of amosite. Most of such cases were identified in recent years, a finding of concern since croc...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Schneider F, Sporn TA, Roggli VL Tags: Ultrastruct Pathol Source Type: journals

Cytoplasmic expression of human telomerase catalytic protein (hTERT) in neutrophils: an immunoelectron microscopy study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human telomerase comprises a catalytic protein subunit (hTERT) and an RNA subunit (hTR). Telomerase extends chromosome ends in compensation for the attrition of the telomeres during replication. In this work, the authors explore the expression of hTERT and hTR in neutrophils, respectively by immunochemistry techniques and in situ hybridization. hTERT was strongly expressed in neutrophils cytoplasm. The ultrastructural study showed that the gold particles were not associated with specific organelles but scattered in the cytosol. hTR was not expressed. hTERT is expressed in the cytoplasm of neutrophils, but its roles-eve...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Lepreux S, Doudnikoff E, Aubert I, Bioulac-Sage P, Bloch B, Martin-Negrier ML Tags: Ultrastruct Pathol Source Type: journals

Microvascular changes in human gastric carcinomas with coagulative necrosis: an ultrastructural study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ultrastructural findings in three cases of gastric carcinoma with coagulative necrosis are reviewed with special emphasis on microvascular changes. Intratumoral microvasculature revealed more or less stabilized vessels. Some were characterized by a close association between pericytes and endothelial cells, whereas others showed laminated basement membrane, with a loose association between pericytes and endothelial cells. Some mural cells exhibited ultrastructural signs of regressive changes, including lipofuscin granules, swollen mitochondria, and cytoplasmic lucency. These findings are discussed in relationship to a n...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Caruso RA, Fedele F, Finocchiaro G, Pizzi G, Nunnari M, Gitto G, Fabiano V, Rigoli L Tags: Ultrastruct Pathol Source Type: journals

New approach for static bone histomorphometry: confocal laser scanning microscopy of maxillo-facial normal bone.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To define the value of confocal laser scanning microscopy (CLSM) as a practical method for a qualitative and quantitative analysis of hard tissue, the authors have analyzed normal maxillo-facial bone. They obtained and analyzed 58 bone samples from 28 patients who underwent to implant surgery. All the samples presented intense autofluorescence primarily ascribed to collagen. Variable degrees of autofluorescence have been identified between osteones and interosteonic bone. CLSM allowed improved tissue imaging, bidimensional pictures with better resolution at cellular level, and, in particular, the possibility of differe...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Pilolli GP, Lucchese A, Maiorano E, Favia G Tags: Ultrastruct Pathol Source Type: journals

Metabolic patterns (NAD(P)H) in rat basophilic leukemia (RBL-2H3) cells and human hepatocellular carcinoma (Hep G2) cells with autofluorescence imaging.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although the spatial and temporal distributions of cellular NAD(P)H concentrations have been theoretically predicted as typical patterns of the metabolism in living cells, so far such a pattern was observed only in neutrophils. In this work, the dynamic NAD(P)H distributions in rat basophilic leukemia (RBL-2H3) and human hepatocellular carcinoma (Hep G2) cells were studied by imaging the autofluorescence of cellular NAD(P)H with a sensitive CCD detector in a confocal microscope. The typical pattern of the cytoplasmic NAD(P)H wave traveling along the long axis of the elongated cell with a velocity of 2.2+/-0.6 mircom/s ...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Chen R, Chen JY, Zhou LW Tags: Ultrastruct Pathol Source Type: journals

4NQO-induced rat tongue carcinoma: an ultrastructural study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinoma, in which the carcinogen is administered systemically in drinking water, is the most comparable animal model to the development of human oral carcinoma. This is the first study to report the ultrastructural changes in this model. The most significant changes were observed in the carcinoma cells at the invasion front and included unique modifications in the basal lamina, presence of micropinocytotic vesicles (plasmalemmal caveolae), and emergence of cytoplasmic microfilaments featuring a parallel arrangement. The microfilaments, in both appearance and orga...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Vered M, Polak-Charcon S, Babushkin T, Dayan D Tags: Ultrastruct Pathol Source Type: journals

Large cell variant ovarian small cell carcinoma: case report.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ultrastructural data about large cell variant ovarian small cell carcinoma (LCV-SCC) are scarce and contradictory and the role of transmission electronmicroscopy (TEM) is not clear in the assessment of such tumors. The authors present a case of LCV-SCC without hypercalcemia in a 30-year-old woman. The diagnosis was confirmed by histopathological and immunohistochemical studies. Cytopathological examination of peritoneal washing showed a population of large neoplastic cells. TEM demonstrated that the neoplasia comprised two types of cells: one type showed many coarse secretory granules without dense core, and the other ...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Donato G, Conforti F, Zuccala V, Cosco C, Laratta C, Perrotta I, Tripepi S, Amorosi A Tags: Ultrastruct Pathol Source Type: journals

Ultrastructure of Kaposi sarcoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study revealed a spectrum of endothelial cell ultrastructure from lymphatic to blood vascular. It demonstrated frequent Weibel-Palade bodies and gap junctions. The spindle cells were shown to behave as facultative phagocytes, internalizing and processing necrotic cells and leaked red blood cells (RBCs). Fragmented RBCs were equivalent to the "hyaline droplets" seen by light microscopy. The final stages of RBC disintegration were hemosiderin and ferritin. Most significantly, this study disclosed that KS is actually composed of a single type of randomly oriented spindle cell forming vessels of varying size and integrity...
Source: Ultrastructural Pathology - September 1, 2008 Category: Pathology Authors: Orenstein JM Tags: Ultrastruct Pathol Source Type: journals

Tubuloreticular structures in different types of myositis: implications for pathogenesis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In dermatomyositis (DM) there is strong histopathological evidence of a microvascular pathogenesis, including endothelial microtubular inclusions. In nonspecific myositis, perimysial and perivascular infiltrates in the muscle biopsy similar to DM are found. Microtubular inclusions in endothelial cells were systematically searched for and found in 4 of the 20 muscle biopsies of nonspecific myositis patients (20%). Three had a CTD (SLE, scleroderma, and Sjogren syndrome). Ten patients with DM and 5 patients with sporadic inclusion body myositis served as positive and negative controls, respectively. PMID: 18696397 [P...
Source: Ultrastructural Pathology - July 1, 2008 Category: Pathology Authors: Bronner IM, Hoogendijk JE, Veldman H, Ramkema M, van den Bergh Weerman MA, Rozemuller AJ, de Visser M Tags: Ultrastruct Pathol Source Type: journals

Peripheral blood smears of myelodysplasia patients: scanning electron microscope findings.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The utility of scanning electron microscopy in the evaluation of ordinary glass peripheral blood smears of patients with myelodysplasia and those uncertain for myelodysplasia is emphasized. Attention is directed to changes in segmented granulocytes. Comparison of ultrastructural findings in abnormal blood smears with control cases is made. Important findings include reduced cytoplasmic granule number, increased cell size, large cytoplasmic vacuoles, condensation of the peripheral cytoplasm, prominence of large cytoplasmic granules, irregular cytoplasmic perimeter, abnormal nuclear morphology, abnormal cell shape, and a...
Source: Ultrastructural Pathology - July 1, 2008 Category: Pathology Authors: Terzakis JA, Taskin M Tags: Ultrastruct Pathol Source Type: journals

Proliferative activity in oral carcinomas studied with Ag-NOR and electron microscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was conducted on 61 patients (27 males and 34 females). The age ranged from 27 to 81 years (mean 54 years). All suffered from oral squamous cell carcinoma and were treated by surgery and deep X-ray therapy (DXT). The UICC and TNM classification and staging recommendations were used for evaluation of the patients. All sections were stained with Ag-NORs stain for examination of the proliferative activity of the squamous cell carcinomas. Biopsies were also taken from another 6 cases--3 cases with normal striated muscle and 3 cases from normal oral mucosa--and served as controls. Statistical studies of Ag-NOR scores...
Source: Ultrastructural Pathology - July 1, 2008 Category: Pathology Authors: Kummoona R, Jabbar A, Kareem Al-Rahal D Tags: Ultrastruct Pathol Source Type: journals