Changes in surfactant protein A and D in ovine ovaries related to follicle development
AbstractSurfactant protein A (SP-A) and Surfactant protein D (SP-D) glycoproteins play a crucial role in maintaining lung homeostasis and lung host defense. Interestingly, these proteins are also expressed in extra-pulmonary tissues, including the female genital tract. The ovarian tissue, where SP-A and SP-D expression increases with follicular development, may serve as the primary site of defense for this tissue. However, their functions in these tissues are not well understood and are currently an active area of research. Therefore, the objective of this study is to investigate the expression of SP-A and SP-D in the ovin...
Source: Veterinary Research Communications - April 18, 2024 Category: Veterinary Research Source Type: research
Correlation between erythrocyte parameters and iron status in cyclic Spanish Purebred mares
AbstractThe aim of this study was to evaluate the serum iron status and its relation to hematological indexes in cyclic mares. Blood samples were taken from 40 Spanish Purebred mares on days − 5, 0, + 5 and + 16 of their cycle. Concentration of transferrin (TRF) was significantly lower on day 0 than on days + 5 and + 16, transferrin saturation (TSAT) decreased significantly on days 0 and + 16 compared to day − 5, total iron-binding capacity (TIBC) on day + 16 was significantly higher than those on days − 5 and 0, and on day + 5 it was also significantly higher than that on day 0, unsaturated...
Source: Veterinary Research Communications - April 18, 2024 Category: Veterinary Research Source Type: research
Pathological findings in South American camelids presented at a farm animal clinic in Northern Germany (2005 –2021)
This study provides an overview of common pathologies in South American camelids in Germany and thus may help to recognise different disease symptoms at an early stage. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - April 17, 2024 Category: Veterinary Research Source Type: research
The vehicle of administration, feed or water, and prandial state influence the oral bioavailability of amoxicillin in piglets
AbstractFeed and water components may interact with drugs and affect their dissolution and bioavailability. The impact of the vehicle of administration (feed and water) and the prandial condition of weaner piglets on amoxicillin ´s oral bioavailability was evaluated. First, amoxicillin’s in vitro dissolution and stability in purified, soft, and hard water, as well as release kinetics from feed in simulated gastric and intestinal media were assessed. Then, pharmacokinetic parameters and bioavailability were determined in fasted and fed pigs using soft water, hard water, or feed as vehicles of administration following a b...
Source: Veterinary Research Communications - April 17, 2024 Category: Veterinary Research Source Type: research
A case report of acute claudication due to vena cava thromboembolism in a dog naturally infected with Leishmania Infantum
AbstractCanine leishmaniosis is a vector-borne disease caused byLeishmania infantum, and clinical manifestations of infection range from absent or severe to fatal and result from immune-mediated mechanisms. In dogs, the most common clinical signs of leishmaniosis include skin lesions and lymphadenomegaly. However, the presence of other nontypical signs has been described, and diagnosing these cases can be challenging. The aim of the present short communication was to describe the impact of the formation of circulating immunocomplexes due toL. infantum in a dog with leishmaniosis affected by a massive venous thrombus of the...
Source: Veterinary Research Communications - April 17, 2024 Category: Veterinary Research Source Type: research
Estrogen receptors alpha and beta expression in different canine cancer types with an emphasis on hematopoietic malignancies
AbstractEstrogen receptors (ERs) are located in both healthy and neoplastic tissues. The type of estrogen receptor expressed varies depending on its location, tumor type, and species. Estrogen action is mediated by binding to ER and activating the transcriptional and signaling processes that result in the control of gene expression. There are two main types of estrogen receptors: ER alpha (ER α) and ER beta (ERβ). Both receptors are functionally different, they may act antagonistically and are distributed in different tissues but their structure is similar – as they are composed of 5 different domains: A/B, C, D, E, an...
Source: Veterinary Research Communications - April 10, 2024 Category: Veterinary Research Source Type: research
Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples
AbstractThe isolation ofCampylobacter fetus subsp.venerealis (Cfv) from clinical samples is the gold standard for confirming cases of bovine genital campylobacteriosis, an important cause of infertility in cattle and a potential public health concern. Furthermore, isolation is also necessary for the development of autologous vaccines, characterization of strains for antimicrobial susceptibility patterns, etc. Nevertheless, the sensitivity of culture methods is usually low, and there is no standardized protocol to maximize the recovery of Cfv from clinical samples. The aim of the current study is to design a protocol for th...
Source: Veterinary Research Communications - April 10, 2024 Category: Veterinary Research Source Type: research
SARS-CoV-2 Mu variant in dogs visiting veterinary clinics during the third pandemic peak in Eastern Colombia
AbstractThe global presence of SARS-CoV-2 in household pets is acknowledged, yet documentation remains scarce, leaving many regions unexplored. Thus, our study sought to fill this gap by investigating SARS-CoV-2 presence in dogs visiting veterinary clinics during the third pandemic peak in eastern Colombia. We collected and analyzed 43 oropharyngeal and rectal swabs using real-time PCR assays targeting the Envelope Gene of SARS-CoV-2. Out of these, two dogs tested positive, indicating an infection rate of 4.7%. Further examination through complete sequencing and phylogenetic analysis revealed the lineage B.1.621 for the SA...
Source: Veterinary Research Communications - April 10, 2024 Category: Veterinary Research Source Type: research
Molecular epidemiology of anaplasmosis in Indian dromedary camels
AbstractThe present study aimed to investigate the molecular epidemiology, risk factors, and haemato-biochemical changes in anaplasmosis in Indian dromedary camels. Blood samples collected from 103 camels were analysed using blood smear examination and semi-nested PCR based on the 16s rRNA gene to diagnose anaplasmosis. The prevalence of anaplasmosis was estimated to be 42.72% (95% CI: 33.59 –52.37) by PCR assay and 16.5% (95% CI: 10.47–24.95%) by blood smear examination. Phylogenetic analysis of six partial sequences of 16s rRNA gene obtained in the present study indicated the involvement of multipleAnaplasma species,...
Source: Veterinary Research Communications - April 9, 2024 Category: Veterinary Research Source Type: research
Comparison of two serological diagnosis tests for bovine paratuberculosis
AbstractMycobacterium avium subsp.paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an “in-house” ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5–9%) and low (≤ 0.05%) prevalenc...
Source: Veterinary Research Communications - April 5, 2024 Category: Veterinary Research Source Type: research
Diffuse large B-cell lymphoma with DNA copy number changes in a Japanese black calf
AbstractA 2-month-old Japanese Black calf exhibited mandibular and superficial cervical lymph node swelling. Fine needle aspiration cytology of the superficial cervical lymph node revealed large lymphoblast-like cells with mitoses. Hematological examination revealed remarkable lymphocytosis with atypical lymphocytes. Increased activities of serum total lactate dehydrogenase and thymidine kinase were detected. At necropsy, generalized swelling of lymph nodes was observed. Histopathological analysis revealed diffuse proliferation of medium-sized round centroblastic neoplastic cells that were positive for CD20, CD79 α, PAX5,...
Source: Veterinary Research Communications - April 5, 2024 Category: Veterinary Research Source Type: research
Green synthesis and anthelmintic activity of silver nanoparticles using Morus Alba Fruit extract against different stages of equine strongyles
In conclusion, GRAgNPs had more robust anthelminthic effects than the standard antiparasitic and CAgNPs. They could be considered as a promising antiparasitic agent. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - April 3, 2024 Category: Veterinary Research Source Type: research
Prevalence and molecular characterization of Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis in dairy cattle in Ningxia, northwestern China
This study aimed to evaluate the zoonotic potential of these three protozoa. In this study, small subunit ribosomal RNA (SSU rRNA) and 60 kDa glycoprotein (gp60) genes ofCryptosporidium; internal transcribed spacer (ITS) gene ofE. bieneusi; and SSU rRNA, glutamate dehydrogenase (gdh), triosephosphate isomerase (tpi), and beta-giardin (bg) genes ofG. duodenalis were examined. DNA extraction, polymerase chain reaction, and sequence analysis were performed on fecal samples collected from 320 dairy cattle at three intensive dairy farms in Ningxia in 2021 to determine the prevalence and genetic characteristics of these three pr...
Source: Veterinary Research Communications - April 3, 2024 Category: Veterinary Research Source Type: research
The continuous surprises that SARS-CoV-2 presents to the scientific community – Correspondence about the Paper Fusco G, Cardillo L, Levante M, Brandi S, Picazio G, Napoletano M, Martucciello A, Fiorito F, De Carlo E, De Martinis C. First serological evidence of SARS-CoV-2 natural infection in small ruminants: brief report. Vet Res Commun. 2023 sep;47(3):1741–1748. Doi: 10 .1007/s11259-022-10044-3
(Source: Veterinary Research Communications)
Source: Veterinary Research Communications - April 1, 2024 Category: Veterinary Research Source Type: research
First study in the frequency of isolation and phenotypic antimicrobial resistance profiles of pig and cattle origin Campylobacter strains in Romania
AbstractCampylobacter spp. is recognized as one of the most common pathogens involved in the development of gastrointestinal infections in humans. The current study aimed to enhance the knowledge on the occurrence and molecular characterization ofCampylobacter spp. in pigs and cattle origin caecum samples (n = 56) collected in one year, from nine Romanian slaughterhouses, and to determine the antimicrobial resistance profile of the isolated strains. AllCampylobacter spp. strains (n = 41) isolated from swine and cattle caecum samples were analyzed in terms of antimicrobial resistance, in accordance with the EURL pro...
Source: Veterinary Research Communications - April 1, 2024 Category: Veterinary Research Source Type: research
