Virchows Archiv
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Establishment and characterization of a novel human malignant peripheral nerve sheath tumor cell line, FMS-1, that overexpresses epidermal growth factor receptor and cyclooxygenase-2
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Abstract Malignant peripheral nerve sheath tumor (MPNST) is a rare soft tissue sarcoma. We established a new human MPNST cell line
(designated FMS-1) from MPNST of the right brachial plexus of a 69-year-old woman with NF1. The cell line has been maintained
for >24 months with >100 passages. FMS-1 cells showed a fibrosarcoma-like or epithelioid pattern in the heterotransplanted
tumor, compared with a fascicular growth pattern of short-spindle tumor cells in the primary tumor. Immunophenotypically,
FMS-1 cells showed almost the same characteristics as the primary tumor. Cytogenetic and molecular ana...
Source: Virchows Archiv - November 17, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Expression of MMP-10, MMP-21, MMP-26, and MMP-28 in Merkel cell carcinomaEmail this article to a colleague.
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We examined by immunohistochemistry
the expression of three novel matrix metalloproteinases (MMPs)—MMP-21, MMP-26, and MMP-28—in 44 primary MCC tumors and six
lymph node metastases while MMP-10 served as a positive control. Their mRNA expression was also studied in the UISO MCC cell
line basally and after various stimulations using quantitative real-time PCR. MMP-28 was observed in tumor cells of 15/44
samples especially in tumors <2 cm in diameter (p = 0.015) while 21/44 specimens showed MMP-28 in the tumor stroma. Expression of MMP-21 was demonstrated in tumor cells of
13/43 samples. MMP-26, instead,...
Source: Virchows Archiv - November 17, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Prognostic implications of CpG island hypermethylator phenotype in colorectal cancersEmail this article to a colleague.
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In conclusion, the CIMP+/MSI− subtype tends to present with distinct clinicopathological and molecular features
and shows the worst clinical outcome among the four molecular subtypes of CRCs.
Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00428-009-0857-0Authors
Jung Ho Kim, Seoul National University College of Medicine Department of Pathology 28 Yongon-dong, Jongno-gu Seoul 110-744 South KoreaSo Hyun Shin, Seoul National University College of Medicine Laboratory of Epigenetics, Cancer Research Institute and 2nd Stage Brain Korea Seoul South KoreaHyeong Ju Kwon, Seoul National University College ...
Source: Virchows Archiv - November 12, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Soft tissue sarcomas with complex genomic profilesEmail this article to a colleague.
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Abstract Soft tissue sarcomas (STS) with complex genomic profiles (50% of all STS) are predominantly composed of spindle cell/pleomorphic
sarcomas, including leiomyosarcoma, myxofibrosarcoma, pleomorphic liposarcoma, pleomorphic rhabdomyosarcoma, malignant peripheral
nerve sheath tumor, angiosarcoma, extraskeletal osteosarcoma, and spindle cell/pleomorphic unclassified sarcoma (previously
called spindle cell/pleomorphic malignant fibrous histiocytoma). These neoplasms show, characteristically, gains and losses
of numerous chromosomes or chromosome regions, as well as amplifications. Many of them share recurr...
Source: Virchows Archiv - November 12, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Vascular endothelial growth factor C mRNA expression is a prognostic factor in epithelial ovarian cancer as detected by kinetic RT-PCR in formalin-fixed paraffin-embedded tissueEmail this article to a colleague.
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Abstract Vascular endothelial growth factor C (VEGF-C) is a well described chemotactic and growth factor for lymphatic endothelial
cells. Its inhibition leads to suppression of lymphatic and distant metastases in mouse models. In ovarian cancer, the relationship
between VEGF-C expression and tumor behavior has not yet been determined by a quantitative method in vivo. Therefore, we used a new technique of RNA extraction from formalin-fixed paraffin-embedded tissue samples and determined
the expression levels of VEGF-C mRNA in a study group of 97 ovarian cancer patients. Expression levels were correlated with
...
Source: Virchows Archiv - November 12, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Immunosuppressive cytokine Interleukin-10 (IL-10) is up-regulated in high-grade CIN but not associated with high-risk human papillomavirus (HPV) at baseline, outcomes of HR-HPV infections or incident CIN in the LAMS cohortEmail this article to a colleague.
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Abstract Bypassing the local immunological defense reactions in the cervix is one of the prerequisites for human papillomaviruses (HPV)
infections to progress to intraepithelial neoplasia (CIN). The role of potent immunosuppressive cytokines, e.g., interleukin-10
(IL-10), depressing these local virus-specific immunological responses is incompletely studied. To assess, whether IL-10 expression
in cervical HPV lesions has any implications in the outcome of HPV infections or disease progression to CIN. Baseline cervical
biopsies from 225 women of the LAMS study sub-cohort were analyzed for IL-10 expression usin...
Source: Virchows Archiv - November 11, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Colonic carcinoma with a pancreatic acinar cell differentiation. A case reportEmail this article to a colleague.
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Abstract A case of a colonic carcinoma showing a pancreatic acinar cell differentiation is described for the first time. A 65-year-old
woman underwent surgical resection for an ulcerated protruding tumour of 4 × 2.5 cm in size on the anterior wall of the sigmoid
colon. Histologically, tumour cells were organized in acinar structures resembling pancreatic acini and in solid nests and
ribbons or diffusely infiltrated as poorly cohesive cells. Lymph nodes and femur metastases displayed the same histological
features. The ultrastructural analysis of the primary tumour indicated the presence of zymogen-...
Source: Virchows Archiv - November 11, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Encapsulated apocrine papillary carcinoma of the breast—a tumour of uncertain malignant potential: report of five casesEmail this article to a colleague.
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Abstract Five cases of an unusual encapsulated apocrine papillary tumour are reported. All presented as cystic masses in the breast
of women aged 44–84 years. Imaging studies showed a complex cyst often with one or more mural nodules. The key histological
features are similar to those of classical encapsulated papillary carcinoma in that myoepithelial cells were absent within
the papillary structures and at the periphery of the cyst. All were pure apocrine in type and showed variable degrees of cytological
atypia and mitotic activity. All lacked evidence of malignancy in the breast tissue outside of t...
Source: Virchows Archiv - October 27, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Precursors of endometrial and ovarian carcinomaEmail this article to a colleague.
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Abstract This review discusses precursor lesions of endometrial and ovarian carcinoma with an emphasis on the unique molecular alterations
that have led to the development of binary classification schemes for tumors of both the endometrium and ovary. While such
a system is well established for endometrial carcinoma, only recently has a binary classification scheme been proposed for
ovarian carcinoma. For both, the morphologic and molecular genetic-defining characteristics of their respective precursor
lesions are described in detail. Furthermore, similarities and differences of the precursor lesions of speci...
Source: Virchows Archiv - October 26, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Aberrant expression of p27Kip1-interacting cell-cycle regulatory proteins in ovarian clear cell carcinomas and their precursors with special consideration of two distinct multistage clear cell carcinogenetic pathwaysEmail this article to a colleague.
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In this study, the expressions of p27Kip1-interacting proteins, i.e., p27Kip1, Skp2, Cks1, cyclin A, cyclin E, and the Ki-67 labeling index (LI), were analyzed in 25 CCAFs (11 benign and 14 borderline)
and 15 CCAF-associated CCAs, and compared with the expression status of each protein in the 23 previously studied endometriosis-associated
CCAs. Although aberrant expression of all p27Kip1-interacting proteins was more frequent in the CCAF-associated CCAs than in the benign CCAFs, statistical significance was
found only for Cks1 overexpression. The frequencies of p27Kip1 downregulation and overexpression of Skp2 and cycli...
Source: Virchows Archiv - October 23, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Genome-scale approaches to the epigenetics of common human diseaseEmail this article to a colleague.
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Abstract Traditionally, the pathology of human disease has been focused on microscopic examination of affected tissues, chemical and
biochemical analysis of biopsy samples, other available samples of convenience, such as blood, and noninvasive or invasive
imaging of varying complexity, in order to classify disease and illuminate its mechanistic basis. The molecular age has complemented
this armamentarium with gene expression arrays and selective analysis of individual genes. However, we are entering a new
era of epigenomic profiling, i.e., genome-scale analysis of cell-heritable nonsequence genetic change, s...
Source: Virchows Archiv - October 21, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Localization of indoleamine 2,3-dioxygenase in human esophageal squamous cell carcinomasEmail this article to a colleague.
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In this study, we have therefore investigated the cellular localization of IDO in
45 ESSCs and ten morphologically normal esophageal tissues; the correlation of IDO with clinicopathological parameters was
also analyzed. Immunohistochemistry (IHC) analysis revealed that the density of IDO-positive cells was increased in ESCCs
relative to controls (P < 0.01). These cells were distributed as clusters and formed a patchy pattern in both the cancerous epithelium and the surrounding
noncancerous cells. Double IHC further confirmed that many IDO-positive cells in the tumor stroma were smooth-muscle-actin-alpha-positive...
Source: Virchows Archiv - October 21, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Histological heterogeneity of Ewing’s sarcoma/PNET: an immunohistochemical analysis of 415 genetically confirmed cases with clinical supportEmail this article to a colleague.
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In conclusion, the diagnosis of atypical ES is a challenge for the pathologist and needs support from molecular techniques
to perform an optimal differential diagnosis with other small round cell tumors.
Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00428-009-0842-7Authors
Antonio Llombart-Bosch, University of Valencia Department of Pathology Ave. Blasco Ibañez, 17 46010 Valencia SpainIsidro Machado, University of Valencia Department of Pathology Ave. Blasco Ibañez, 17 46010 Valencia SpainSamuel Navarro, University of Valencia Department of Pathology Ave. Blasco Ibañez, 17 46010 Valencia SpainF...
Source: Virchows Archiv - October 20, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Full analysis of the prostatic urethra at the time of radical cystoprostatectomy for bladder cancer: impact on final disease stageEmail this article to a colleague.
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Abstract Prostate involvement is a major prognostic element in urothelium carcinoma staging after radical cystoprostatectomy (RCP)
for muscle invasive bladder cancer. While appropriate pTNM stage is necessary for adequate treatment, no standard procedure
exists up to now for macroscopic sampling of the prostate in RCP in daily practice. We therefore propose a protocol where
examination of the whole prostatic urethra (PU) is possible, without using whole mount sections. From 2008 to June 2009, RCP
were sampled according to a macroscopic protocol permitting the whole length and the underlying stroma of PU to b...
Source: Virchows Archiv - October 20, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Overexpression of Aurora B is associated with poor prognosis in epithelial ovarian cancer patientsEmail this article to a colleague.
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Abstract Recent studies have indicated that Aurora B expression is related to cell proliferation and prognosis in many cancers, but
its association with epithelial ovarian carcinoma is not fully understood. Therefore, we examined the Aurora B kinase expression
in epithelial ovarian cancer patients. Using immunohistochemistry, the expression levels of Aurora B and phosphohistone H3
(Ser10) (mitosis-specific marker) were measured in 156 patients with epithelial ovarian cancer. The expression levels of Aurora
B at the protein and messenger RNA levels were examined using Western blotting and reverse transcriptas...
Source: Virchows Archiv - October 16, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Metastatic potential of an aneurysmal bone cystEmail this article to a colleague.
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Abstract Aneurysmal bone cysts (ABCs) are benign bone tumors consisting of blood-filled cavities lined by connective tissue septa.
Recently, the hypothesis that ABCs are lesions reactive to local hemodynamics has been challenged after the discovery of specific
recurrent chromosomal abnormalities. Multiple cases of malignant transformation of ABC into (osteo)sarcoma have been described,
as well as a number of cases of telangiectatic osteosarcoma which had been misdiagnosed as ABC. We herewith document a case
of a pelvic ABC metastatic to the lung, liver, and kidneys. Diagnosis was confirmed by the presence of...
Source: Virchows Archiv - October 16, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Pathologic characteristics of resected squamous cell carcinoma of the trachea: prognostic factors based on an analysis of 59 casesEmail this article to a colleague.
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Abstract While squamous cell carcinoma (SCC) is the most common tracheal malignancy, few reports describe the pathologic considerations
that may guide intraoperative decisions and prognostic assessment. We reviewed 59 tracheal SCC treated between 1985 and 2008
by segmental resection of the trachea, including resection of the carina in 24% and inferior larynx in 14%. We classified
these tumors by grading histologic differentiation and microscopic features used in SCC of other sites. Of 59 tumors, 24%
(14 of 59) were well differentiated, 49% (29 of 59) were moderately differentiated, and 27% (16 of 59) were po...
Source: Virchows Archiv - October 16, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Acute and chronic placental membrane hypoxic lesionsEmail this article to a colleague.
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Abstract Laminar necrosis (LN) and microscopic chorionic pseudocysts (MCP) are the two histological placental membrane lesions. This
study retrospectively compares the clinical and placental associations of LN and MCP on a large placental material. Four hundred
seventy-nine placentae featuring membrane LN (group 1), 220 placentae with MCP (group 2), and 50 placentae with both LN and
MCP were identified in the database of consecutively signed by the author 4,853 placentae from 18 to 42 weeks pregnancies
in years 1994–2007. Frequencies or averages of several clinical conditions and gross and microscopic...
Source: Virchows Archiv - October 14, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
The term cor bovinum in anatomical pathologyEmail this article to a colleague.
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Content Type Journal ArticleCategory Letter to the EditorDOI 10.1007/s00428-009-0839-2Authors
Georg Reith, University of Gießen Department of Legal Medicine Frankfurter Straße 58 35392 Gießen GermanyBernd Busch, University of Gießen Department of Legal Medicine Frankfurter Straße 58 35392 Gießen GermanyMarcel A. Verhoff, University of Gießen Department of Legal Medicine Frankfurter Straße 58 35392 Gießen Germany
Journal Virchows ArchivOnline ISSN 1432-2307Print ISSN 0945-6317 (Source: Virchows Archiv)
Source: Virchows Archiv - October 13, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Atypical lipomatous tumors of the tongue: report of six casesEmail this article to a colleague.
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We report six new cases of atypical
lipomatous tumor (ALT) of the tongue, and detection of mdm-2 and CDK4 expression by immunohistochemistry and fluorescence
in situ hybridization (FISH), respectively, was performed. The series comprised three males and three females, aged 11–78 years.
The tumors arose at the lateral side of the tongue, and in one case, multiple tumor nodules were noted. Follow-up information
in five cases (range from 4 to 159 months) revealed one local recurrence at 6 months. Microscopically, four cases had features
of lipoma-like ALT, whereas two cases displayed patterns of sclerosi...
Source: Virchows Archiv - October 9, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Nodular pattern of bone marrow infiltration: frequent finding in immunosuppression-related EBV-associated large B-cell lymphomasEmail this article to a colleague.
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Abstract Different patterns of bone marrow (BM) infiltration by diffuse large B cell lymphomas (DLBCL) have been described. A pure
nodular pattern is uncommon, and the pathologic features, as well as the clinical correlates of DLBCL manifesting this pattern
in the BM have not been well characterized. We evaluated BM biopsies involved by large B cell lymphomas diagnosed at our institute
over an 11-year period to assess the morphology, phenotype, cytogenetic abnormalities, and clinical features of cases associated
with a nodular pattern. A distinct nodular pattern of BM involvement was noted in 14 out of 55 (2...
Source: Virchows Archiv - October 6, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Transgenic mice expressing nitroreductase gene under the control of the podocin promoter: a new murine model of inductible glomerular injuryEmail this article to a colleague.
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Abstract The present work identifies a new mouse model of inductible acute glomerular injury leading to focal segmental glomerulonephritis.
We take advantage of the suicide gene/prodrug nitroreductase/CB1954 combination, in which nitroreductase converts CB1954,
a monofunctional alkylating agent, into its toxic form. We generate two lines of transgenic mice in which the nitroreductase
gene was placed under the control of the podocyte-specific gene podocin. The functional analysis of transgenic mice lines
showed that CB1954 treatment induced a severe but transitory proteinuria. Sequential histopathological ana...
Source: Virchows Archiv - October 6, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Molecular pathology of sarcomas: concepts and clinical implicationsEmail this article to a colleague.
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Abstract The molecular genetic changes that have been described in sarcomas over the past era have aided our understanding of their
pathogenesis. The majority of sarcomas carry nonspecific genetic changes within a background of a complex karyotype. These
constitute the challenges in sarcoma research for unraveling a putative multistep genetic model, such as for chondrosarcoma,
and finding targets for therapeutic strategies. Approximately 15–20% of mesenchymal tumors carry a specific translocation
within a relatively simple karyotype. The resulting fusion products act either as transcription factors upregul...
Source: Virchows Archiv - September 29, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
The importance of precise pT diagnosis for prognostic prediction of uterine cervical cancer—a single institutional report at a Japanese comprehensive cancer hospitalEmail this article to a colleague.
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Abstract We previously reported that the majority of Japanese pathologists misunderstand the International Union against Cancer-pT2
criteria for uterine cervical cancer (UCC). We compared the prognosis of originally diagnosed pT2 (ori-pT) UCC cases at our
hospital with reclassified pT2 (re-pT) cases to assess the importance of making a correct pT diagnosis. There were 43 International
Federation of Gynecology and Obstetrics (FIGO) II (i.e., cT2) and/or ori-pT2 UCC cases who received surgery without neoadjuvant
chemotherapy at Shikoku Cancer Center from 1991 to 2003. The cases (seven ori-pT1 and 36 ori-pT2; 4...
Source: Virchows Archiv - September 24, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Splenic micronodular T-cell/histiocyte-rich large B-cell lymphoma: effect of prior corticosteroid therapyEmail this article to a colleague.
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We report on three patients who were treated with corticosteroids only prior to the diagnosis of splenic lymphoma. Corticosteroids
were administered for different conditions, at different doses, and for various periods of time. The primary diagnosis was
splenic micronodular T-cell/histiocyte-rich large B-cell lymphoma in the three cases, and it was reached with variable difficulty.
We suggest that the corticosteroid treatment was one of the causes for the complications in reaching a diagnosis. The morphologic
appearance of the microscopic splenic nodules was the most variable feature and may possibly reflect the dose a...
Source: Virchows Archiv - September 18, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
CpG island hypermethylation and repetitive DNA hypomethylation in premalignant lesion of extrahepatic cholangiocarcinomaEmail this article to a colleague.
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The objective of this study was to identify the occurrence of CpG island hypermethylation and repetitive DNA hypomethylation
in BilIN. A total of 50 EHCs, 31 BilINs, and 31 normal cystic duct samples were analyzed for their methylation status in seven
genes and two repetitive DNA elements. The number of methylated genes increased with disease progression (normal bile duct,
0.6; BilIN, 2.0; EHC, 3.6; P < 0.001). The methylation level of examined genes was significantly higher in BilIN than in normal samples (TMEFF2, HOXA1, NEUROG1, and RUNX3, P < 0.05) and in EHC than in BilIN samples (TMEFF2, HOXA1, NEUROG...
Source: Virchows Archiv - September 18, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
p16INK4 and CEA can be mutually exchanged with confidence between both relevant three-marker panels (ER/Vim/CEA and ER/Vim/p16INK4) in distinguishing primary endometrial adenocarcinomas from endocervical adenocarcinomas in a tissue microarray studyEmail this article to a colleague.
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Abstract The accurate distinction between primary endocervical adenocarcinomas (ECA) and endometrial adenocarcinomas (EMA) may require
the use of multiple ancillary monoclonal antibodies in panels of immunohistochemistry stains. In addition to reappraising
the expressions of four commonly used individual monoclonal antibodies [estrogen receptor (ER), Vimentin (Vim), carcinoembryonic
antigen (CEA), and p16INK4], this study was designed to investigate whether CEA and p16INK4 can be effectively exchanged between two relevant three-marker panels (ER/Vim/CEA vs. ER/Vim/p16INK4) in distinguishing ECA from EMA. A ti...
Source: Virchows Archiv - September 18, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Intra- and interobserver reproducibility of interpretation of immunohistochemical stains of prostate cancerEmail this article to a colleague.
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Abstract The evaluation of immunohistochemistry (IHC) is usually semiquantitative, and thus subject to observer variability. We analyzed
the reproducibility of different IHC measures. Fifty TMA cores of prostate cancer were stained for PDX-1, a transcription
factor overexpressed in the cytoplasm of prostate cancer cells. The strongest intensity was scored 0–3 and 1–3 was used for
extent (1–33%, 34–66%, and 67–100%). The stains were evaluated twice by four observers: two genitourinary pathologists, and
two medical doctors with no formal pathology training. Staining intensity was also measured with a...
Source: Virchows Archiv - September 17, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Tissue transglutaminase expression in celiac mucosa: an immunohistochemical studyEmail this article to a colleague.
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Abstract Tissue transglutaminase (tTG) constitutes the main autoantigen in celiac disease (CD). The aim of the study was to clarify
weather celiac disease is associated with changes in tTG expression in duodenal mucosa. Tissue transglutaminase was assessed
immunohistochemically (clone CUB 7402) in duodenal biopsy specimens from 22 untreated CD patients, ten normal controls (NC)
with unremarkable duodenal mucosa, and nine disease nonceliac controls (DC). In 15 CD patients duodenal biopsy specimens were
repeatedly assessed after these patients had been prescribed gluten-free diet. Positive pixel count algorith...
Source: Virchows Archiv - September 16, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Intestinal type of mucinous borderline tumor arising from mixed epithelial and stromal tumor of kidneyEmail this article to a colleague.
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We report a mucinous borderline tumor arising from a mixed epithelial and stromal tumor of left kidney (MESTK). The patient
was an 82-year-old woman who presented with gross hematuria and recurrent urinary tract infection for years. The patient had
a cytoscopy with a retrograde pyelogram, which indicated a dilated left kidney with a central mass lesion. Subsequently, she
underwent a radical left nephrectomy. Cross-sections of left kidney showed a 4.5 × 3.5 × 1.5 cm ill-defined cystic lesion
with mucinous and solid areas. Histologic sections of the lesion showed numerous variable-sized dilated cysts with ...
Source: Virchows Archiv - September 16, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Prevalence, viral load, and physical status of HPV 16 and 18 in cervical adenosquamous carcinomaEmail this article to a colleague.
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Abstract Adenosquamous carcinoma of the uterine cervix is a rare mixture of malignant squamous and glandular epithelial elements and
accounts for approximately 10% of cervical carcinomas. The aims of the present study were to evaluate the prevalence, physical
status, and viral load of HPV 16 and 18 in adenosquamous carcinoma. Formalin-fixed paraffin-embedded tissue samples from 20
cases of histologically diagnosed adenosquamous carcinoma were examined. The squamous and glandular components were separately
microdissected and analyzed for their HPV DNA subtype, viral load, and physical status using real-time p...
Source: Virchows Archiv - September 3, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Neutrophil gelatinase-associated lipocalin (NGAL/Lcn2) is upregulated in gastric mucosa infected with Helicobacter pyloriEmail this article to a colleague.
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In conclusion, the high expression of NGAL/Lcn2 in normal and gastritis-affected mucosa infected with H. pylori suggests that NGAL/Lcn2 is upregulated locally in response to this bacterial infection. It is discussed whether this may
have a causal relation to the development of gastric cancer.
Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00428-009-0825-8Authors
Warner Alpízar-Alpízar, Haukeland University Hospital The Gade Institute, University of Bergen and Department of Pathology Bergen NorwayOle Didrik Laerum, Haukeland University Hospital The Gade Institute, University of Bergen and Departme...
Source: Virchows Archiv - September 3, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Diffuse large B-cell lymphoma with a high number of epithelioid histiocytes (lymphoepithelioid B-cell lymphoma): a study of Osaka Lymphoma Study GroupEmail this article to a colleague.
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Abstract The aim of this study was to clarify whether diffuse large B-cell lymphoma (DLBCL) with a high number of epithelioid histiocytes
(DLBCL-EH) could have distinctive clinicopathological characteristics. Clinicopathological findings in 22 cases with DLBCL-EH
and, as a control, 96 cases with ordinary type of DLBCL were analyzed. There were ten men and 12 women with ages ranging from
38 to 91 (median, 64) years. The primary site was lymph node in 16 cases, extranodal organs in three, and unknown in three.
Stage of disease was I in five cases, II in three, III in nine, and IV in five. Histologically, there...
Source: Virchows Archiv - September 3, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Running GAGs: myxoid matrix in tumor pathology revisitedEmail this article to a colleague.
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Abstract Ever since Virchow introduced the entity myxoma, abundant myxoid extracellular matrix (ECM) has been recognized in various
reactive and neoplastic lesions. Nowadays, the term “myxoid” is commonly used in daily pathological practice. But what do
today’s pathologists mean by it, and what does the myxoid ECM tell the pathologist? What is known about the exact composition
and function of the myxoid ECM 150 years after Virchow? Here, we give an overview of the composition and constituents of the
myxoid ECM as known so far and demonstrate the heterogeneity of the myxoid ECM among different tumo...
Source: Virchows Archiv - August 24, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Loss of RKIP expression is associated with poor survival in GISTsEmail this article to a colleague.
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Abstract Gastrointestinal stromal tumours (GISTs) are rare mesenchymal tumours of the digestive tract and are commonly driven by oncogenic
mutations in KIT and PDGFRA genes. Tumour size, location, mitotic index and KIT/PDGFRA mutations are the most important prognostic parameters in GISTs. However, additional studies screening for new molecular
prognostic markers in GISTs are missing. Raf kinase inhibitor protein (RKIP) has been considered as a suppressor of metastasis
and a prognostic marker in several neoplasms. In the present study we aimed to examine whether RKIP expression is associated
with GIST clinic...
Source: Virchows Archiv - August 24, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
High resolution analysis of DNA copy-number aberrations of chromosomes 8, 13, and 20 in gastric cancersEmail this article to a colleague.
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Abstract DNA copy-number gains of chromosomes 8q, 13q, and 20q are frequently observed in gastric cancers. Moreover gain of chromosome
20q has been associated with lymph node metastasis. The aim of this study was to correlate DNA copy-number changes of individual
genes on chromosomes 8q, 13q, and 20q in gastric adenocarcinomas to clinicopathological data. DNA isolated from 63 formalin-fixed
and paraffin-embedded gastric adenocarcinoma tissue samples was analyzed by whole-genome microarray comparative genomic hybridization
and by multiplex ligation-dependent probe amplification (MLPA), targeting 58 individual...
Source: Virchows Archiv - August 21, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Immunohistochemical expression of ubiquitin and telomerase in cervical cancerEmail this article to a colleague.
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Abstract Ubiquitin and telomerase immunohistochemical expression patterns in cervical cancer were compared with normal cervical tissue
samples. Eighty-one cervical cancer cases and 22 normal exo–endocervical tissue were examined with polyclonal antibody for
ubiquitin and 44G12 clone for telomerase using tissue microarrays. The results were interpreted using a semiquantitative scale
The average age of patients was 50.67 years. The most frequent histological types were moderately differentiated epidermoid
carcinoma (43.5%), according to the degree of differentiation, and endocervical adenocarcinoma (42....
Source: Virchows Archiv - August 19, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Well-differentiated and dedifferentiated liposarcomasEmail this article to a colleague.
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Abstract Atypical lipomatous tumor or well-differentiated liposarcoma (ALT-WDLPS) and dedifferentiated liposarcoma (DDLPS) share the
same basic genetic abnormality characterized by a simple genomic profile with a 12q14–15 amplification involving MDM2 gene. These tumors are the most frequent LPS. This paper reviews the molecular pathology, general clinical and imaging features,
histopathology, new diagnostic tools, and prognosis of ALT-WDLPS and DDLPS.
Content Type Journal ArticleCategory Review and PerspectiveDOI 10.1007/s00428-009-0815-xAuthors
Jean-Michel Coindre, Institut Bergonié Department of Path...
Source: Virchows Archiv - August 17, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Hermann Lebert (1813–1878): a pioneer of diagnostic pathologyEmail this article to a colleague.
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Abstract Hermann Lebert (1813–1879) was a pioneer of diagnostic pathology and medical iconography. He was born in Breslau, then Prussia,
and died in Nice (France). He lived in Switzerland as a general physician, in France as a pathologist, and eventually became
the chairman for internal medicine in Zurich and Breslau, respectively. The significance of Hermann Lebert for medical posterity
has three aspects: firstly, scientific linking of the French (Parisian) school and its distinctive clinical/practical orientation
to the later clinical/pathological German school of Johann Lukas Schönlein, Johannes Mülle...
Source: Virchows Archiv - August 17, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
DLK is a novel immunohistochemical marker for adrenal gland tumorsEmail this article to a colleague.
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Abstract Delta-like protein (DLK) is expressed in fetal and adult adrenal glands. We have investigated if this expression is maintained
in adrenal gland-derived tumors. All the studied 37 cortical tumors, including five carcinomas, stained positively as well
as the 13 examined pheochromocytomas. Thus, DLK is a very sensitive marker for adrenal tumors of cortical and medullary origin.
Renal cell carcinomas, presenting the major differential diagnostic problem for cortical tumors, were all negative, as well
as melanomas, which are similar to high portion of adrenocortical tumors that react with melan-A. Howeve...
Source: Virchows Archiv - August 14, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Adhesion molecules and p16 expression in endocervical adenocarcinomaEmail this article to a colleague.
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Abstract An immunohistochemical (IHC) study has been conducted on 34 cases of untreated endocervical adenocarcinomas collected among
three institutions (Ospedale S. Andrea, Rome; Istituto Nazionale Tumori “Fondazione G. Pascale”, Naples; and Clinica Malzoni,
Avellino). The E-cadherin and α- and β-catenin complex status has been investigated along with p16INK4a in all studied cases
with the aim to study whether the pattern of expression of the cadherin–catenin complex could be causally related to the expression
of P16INK4a protein. Results were evaluated for statistical significance by a non-parametri...
Source: Virchows Archiv - August 12, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
A combined histologic and molecular approach identifies three groups of gastric cancer with different prognosisEmail this article to a colleague.
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Abstract The limited prognostic value of currently used histologic classifications of gastric cancer and their failure to account for
the complexity of the disease as revealed by more recent investigations prompted a combined reinvestigation of histologic,
molecular, and clinicopathologic patterns in 294 extensively sampled, invasive gastric cancers representing all main histotypes
and stages of the disease and followed for a median of 150 months. Among histologic parameters tested, only cellular atypia,
angio-lympho- or neuroinvasion, Ki67 proliferation index, expansile/infiltrative type growth, and T8...
Source: Virchows Archiv - August 11, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Fibroadenoma and intraduct papilloma—a common pathogenesis?Email this article to a colleague.
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This report describes two cases, each showing fibroepithelial proliferation, admixed with intraduct papillomas. Case 1 was
from a 13-year-old female who presented with a breast mass, while case 2 was from a 60-year-old female who also had a breast
lump. Case 1 showed proliferative breast disease with multiple small papillomas, some with leaf-like contours. Broad-based
parenchymal proliferations protruding into ducts were also seen, and there were overlap features between the two ends of this
spectrum. Case 2, similarly, demonstrated a mixed intraductal proliferation, with papillomas on narrow stalks together with
low-...
Source: Virchows Archiv - August 11, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
An allelotype analysis indicating the presence of two distinct ovarian clear-cell carcinogenic pathways: endometriosis-associated pathway vs. clear-cell adenofibroma-associated pathwayEmail this article to a colleague.
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Abstract Patterns of allele loss (loss of heterozygosity (LOH)) were studied to identify the genetic backgrounds underlying the two
putative carcinogenic pathways of ovarian clear-cell adenocarcinoma: carcinomas thought to arise in endometriosis (endometriosis-associated
carcinomas, 20 cases) and carcinomas thought to be derived from clear-cell adenofibroma ((CCAF)-associated carcinomas, 14
cases). Each tumor was assessed for LOH at 24 polymorphic loci located on 12 chromosomal arms: 1p, 3p, 5q, 8p, 9p, 10q, 11q,
13q, 17p, 17q, 19p, and 22q. For all informative loci, the frequency of LOH was not statisticall...
Source: Virchows Archiv - August 4, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Clinical relevance of neuroendocrine differentiation in non-small cell lung cancer assessed by immunohistochemistry: a retrospective study on 405 surgically resected casesEmail this article to a colleague.
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In conclusion, additional immunohistochemical
detection of neuroendocrine differentiation in non-small cell lung cancer is presently not of prognostic importance and does
not justify a distinct consideration.
Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s00428-009-0812-0Authors
William Sterlacci, Medical University Innsbruck Institute for Pathology Muellerstrasse 44 6020 Innsbruck AustriaMichael Fiegl, Medical University Innsbruck Division of Haematology and Oncology, Department of Internal Medicine Anichstrasse 35 6020 Innsbruck AustriaWolfgang Hilbe, Medical University Innsbruck Division of Hae...
Source: Virchows Archiv - August 3, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
From virtual microscopy to systems pathologyEmail this article to a colleague.
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Content Type Journal ArticleCategory Meeting ReportDOI 10.1007/s00428-009-0810-2Authors
Niels Grabe, University of Heidelberg Hamamatsu Tissue Imaging and Analysis (TIGA) Center, BIOQUANT, INF 267 Heidelberg GermanyPeter Schirmacher, University of Heidelberg Hamamatsu Tissue Imaging and Analysis (TIGA) Center, BIOQUANT, INF 267 Heidelberg Germany
Journal Virchows ArchivOnline ISSN 1432-2307Print ISSN 0945-6317 (Source: Virchows Archiv)
Source: Virchows Archiv - August 3, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
AbstractEmail this article to a colleague.
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Content Type Journal ArticleCategory AbstractsDOI 10.1007/s00428-009-0805-z
Journal Virchows ArchivOnline ISSN 1432-2307Print ISSN 0945-6317
Journal Volume Volume 455
Journal Issue Volume 455, Supplement 1 / August, 2009 (Source: Virchows Archiv)
Source: Virchows Archiv - July 25, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Overexpression of PTK6 (breast tumor kinase) protein—a prognostic factor for long-term breast cancer survival—is not due to gene amplificationEmail this article to a colleague.
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Abstract In a previous retrospective study, we demonstrated the prognostic value of protein tyrosine kinase 6 (PTK6) protein expression
in breast carcinomas. Here, we analyzed PTK6 gene amplification using fluorescence in situ hybridization technique in a cohort
of 426 invasive breast carcinomas and compared it with PTK6 expression level as well as with the clinical outcome of patients.
Forty-five percent of tumors show increased PTK6 gene copy numbers when compared to normal tissue. Most of these, however,
were related to chromosome 20 polysomy (30%), while gene amplification accounted for only 15%. Only ...
Source: Virchows Archiv - July 23, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
The distribution of lesions in 1–14-mm invasive breast carcinomas and its relation to metastatic potentialEmail this article to a colleague.
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Abstract We analyzed 301 consecutive cases of 1–14-mm invasive breast carcinomas documented in large-format histological sections to
determine the distribution of invasive and in situ foci. We also aimed to determine whether this distribution was related
to the frequency of demonstrable vascular invasion and lymph node metastases. One third of the carcinomas (31.9%, 96 cases)
had a multifocal invasive component and a more than doubled relative risk of vascular invasion (RR = 2.3642, 95% confidence
interval (CI) = 1.5077–3.7073) and lymph node metastasis (RR = 2.7760, 95% CI = 1.6337–4.7...
Source: Virchows Archiv - July 23, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
Lymphangiomatoid pattern in diffuse malignant mesothelioma of the pleura: a report of six casesEmail this article to a colleague.
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Abstract The multiplicity of epithelioid and mesenchymal forms of diffuse malignant mesothelioma includes patterns that may mimic another
process. Identification of the multiplicity of patterns may help in the diagnosis of diffuse malignant mesothelioma. One pattern
that has not been described is lymphangiomatoid. We observed six cases with ovoid or elongated or irregular anastomosing vascular-like
spaces lined by flattened cells simulating lymphangioma. The luminal spaces could contain proteinaceous material simulating
lymph but not erythrocytes. The cells lining the spaces were mesothelial by immunohistoch...
Source: Virchows Archiv - July 18, 2009 Category: Pathology Tags: Virchows Archiv Source Type: journals
