Neutralization sensitivity of SARS-CoV-2 Omicron variants FL.1 and GE.1 by therapeutic antibodies and XBB sera
In this study, we assessed the neutralizing ability of eleven FDA-approved monoclonal antibody combinations against different Omicron variants, including BA.2.75, BA.2.76, BA.4/5, XBB.1.5, and CH.1.1. Among the eleven antibodies, Sotrovimab was the only antibody to show broad neutralization ability against XBB.1.5. However, Sotrovimab showed attenuated neutralization efficiency against recently emerging XBB sub-lineages EG.5, FL.1, and GE.1 compared to XBB.1.5. Additionally, XBB.1.5 seropositive convalescent sera displayed lower neutralization activity against EG.5, FL.1, and GE.1. Overall, our findings present enhanced im...
Source: Virology - April 23, 2024 Category: Virology Authors: Joseph Lee Youichi Naoe Uikyu Bang Yu Nakagama Akatsuki Saito Yasutoshi Kido Akitsu Hotta Source Type: research
CX-6258 hydrochloride hydrate: A potential non-nucleoside inhibitor targeting the RNA-dependent RNA polymerase of norovirus
Virology. 2024 Apr 17;595:110088. doi: 10.1016/j.virol.2024.110088. Online ahead of print.ABSTRACTHuman norovirus (HuNoV), a primary cause of non-bacterial gastroenteritis, currently lacks approved treatment. RdRp is vital for virus replication, making it an attractive target for therapeutic intervention. By application of structure-based virtual screening procedure, we present CX-6258 hydrochloride hydrate as a potent RdRp non-nucleoside inhibitor, effectively inhibiting HuNoV RdRp activity with an IC50 of 3.61 μM. Importantly, this compound inhibits viral replication in cell culture, with an EC50 of 0.88 μM. In vitro b...
Source: Virology - April 21, 2024 Category: Virology Authors: Yang Liu Quanjie Li Huihan Shao Yang Mao Lufei Liu Dongrong Yi Zhaojun Duan Huiqing Lv Shan Cen Source Type: research
GmCYB5-4 inhibit SMV proliferation by targeting P3 protein
In this study, we comprehensively analyzed the GmCYB5 family in soybeans, including its distribution on chromosomes, promoter analysis, conserved motifs, phylogenetic analysis, and expression patterns. We cloned the full-length GmCYB5-4 and examined its interaction with P3 in yeast, which was later confirmed using bimolecular fluorescence complementation (BiFc). We silenced GmCYB5-4 using a bean pottle mosaic viris (BPMV) based system to generate SilCYB5-4 tissues, which surprisingly knocked down four isoforms of GmCYB5s for functional characterization. SilCYB5-4 plants were challenged with the SC3 strain to determine its ...
Source: Virology - April 19, 2024 Category: Virology Authors: Daiqiao Song Kai Huang Shuxin Li Jia Jiang Longgang Zhao Hexiang Luan Source Type: research
Major open questions in the hepatitis B and D field - Proceedings of the inaugural International emerging hepatitis B and hepatitis D researchers workshop
Virology. 2024 Apr 16;595:110089. doi: 10.1016/j.virol.2024.110089. Online ahead of print.ABSTRACTThe early and mid-career researchers (EMCRs) of scientific communities represent the forefront of research and the future direction in which a field takes. The opinions of this key demographic are not commonly aggregated to audit fields and precisely demonstrate where challenges lie for the future. To address this, we initiated the inaugural International Emerging Researchers Workshop for the global Hepatitis B and Hepatitis D scientific community (75 individuals). The cohort was split into small discussion groups and the sign...
Source: Virology - April 19, 2024 Category: Virology Authors: Thomas Tu Jochen Wettengel Yuchen Xia Barbara Testoni Margaret Littlejohn Nina Le Bert Gregor Ebert Eloi R Verrier John E Tavis Chari Cohen Source Type: research
Genome-based characterization of a novel prophage of Vibrio parahaemolyticus, VPS05ph1, a novel member of Peduoviridae
In this study, two prophages (VPS05ph1 and VPS05ph2) were identified in a V. parahaemolyticus isolate through genomic and transcriptional analyses. Both prophages were determined as HP1-like phages, located in a novel phylogenetic lineage of Peduoviridae. They shared a moderate genome-wide sequence similarity with each other and high synteny with the closest relatives, but showed low identities to the repressor counterparts of the representative phages within the family. In addition, no bacterial virulence genes, antibiotic resistance genes and known phage-encoded lytic proteins were identified on both prophage genomes. Mo...
Source: Virology - April 18, 2024 Category: Virology Authors: Hailiang Wang Guosi Xie Jie Huang Source Type: research
Assessment of the neutralizing antibody response in Omicron breakthrough cases in healthcare workers who received the homologous booster of Moderna mRNA-1273
Virology. 2024 Apr 8;595:110082. doi: 10.1016/j.virol.2024.110082. Online ahead of print.ABSTRACTVaccines against SARS-CoV-2 were developed during the pandemic including the BNT162b2 and the mRNA-1273. We evaluated the levels of binding antibodies against the receptor binding domain and the levels of NAbs in individuals who developed a breakthrough infection after having received three doses of mRNA-1273. A total of 51 participants were included. The breakthrough group was compared to a 1:1 matched-control group. Among the 51 individuals, 18 (35%) developed a breakthrough infection. The GMT of NAbs against the BA.1 in the ...
Source: Virology - April 18, 2024 Category: Virology Authors: Constant Gillot Marie Tr é-Hadry Roberto Cupaiolo Laurent Blairon Alain Wilmet Ingrid Beukinga Jean-Michel Dogn é Jonathan Douxfils Julien Favresse Source Type: research
Genome-based characterization of a novel prophage of Vibrio parahaemolyticus, VPS05ph1, a novel member of Peduoviridae
In this study, two prophages (VPS05ph1 and VPS05ph2) were identified in a V. parahaemolyticus isolate through genomic and transcriptional analyses. Both prophages were determined as HP1-like phages, located in a novel phylogenetic lineage of Peduoviridae. They shared a moderate genome-wide sequence similarity with each other and high synteny with the closest relatives, but showed low identities to the repressor counterparts of the representative phages within the family. In addition, no bacterial virulence genes, antibiotic resistance genes and known phage-encoded lytic proteins were identified on both prophage genomes. Mo...
Source: Virology - April 18, 2024 Category: Virology Authors: Hailiang Wang Guosi Xie Jie Huang Source Type: research
Assessment of the neutralizing antibody response in Omicron breakthrough cases in healthcare workers who received the homologous booster of Moderna mRNA-1273
Virology. 2024 Apr 8;595:110082. doi: 10.1016/j.virol.2024.110082. Online ahead of print.ABSTRACTVaccines against SARS-CoV-2 were developed during the pandemic including the BNT162b2 and the mRNA-1273. We evaluated the levels of binding antibodies against the receptor binding domain and the levels of NAbs in individuals who developed a breakthrough infection after having received three doses of mRNA-1273. A total of 51 participants were included. The breakthrough group was compared to a 1:1 matched-control group. Among the 51 individuals, 18 (35%) developed a breakthrough infection. The GMT of NAbs against the BA.1 in the ...
Source: Virology - April 18, 2024 Category: Virology Authors: Constant Gillot Marie Tr é-Hadry Roberto Cupaiolo Laurent Blairon Alain Wilmet Ingrid Beukinga Jean-Michel Dogn é Jonathan Douxfils Julien Favresse Source Type: research
AMPK restricts HHV-6A replication by inhibiting glycolysis and mTOR signaling
In this study, we investigated the role of AMPK in response to human herpesvirus 6A (HHV-6A) infection. We show that HHV-6A infection significantly downregulates the active phosphorylated state of AMPK in infected T cells. Pharmacological activation of AMPK highly attenuated HHV-6A propagation. Mechanistically, we found that the activation of AMPK by AICAR blocked HHV-6-induced glycolysis by inhibiting glucose metabolism and lactate secretion, as well as decreasing expressions of key glucose transporters and glycolytic enzymes. In addition, mTOR signaling has been inactivated in HHV-6A infected T cells by AICAR treatment. ...
Source: Virology - April 17, 2024 Category: Virology Authors: Xiaodi Yang Siyu Tian Zhujiang Min Emanuela Garbarino Jingjing Ma Junli Jia Huamin Tang Lingyun Li Source Type: research
Opportunistic sampling of yellow canary (Crithagra flaviventris) has revealed a high genetic diversity of detected parvoviral sequences
Virology. 2024 Apr 4;595:110081. doi: 10.1016/j.virol.2024.110081. Online ahead of print.ABSTRACTParvoviruses are known to be significant viral pathogens that infect a wide range of species globally. However, little is known about the parvoviruses circulating in Australian birds, including yellow canaries. Here, we present four parvoviral sequences including three novel parvoviruses detected from 10 yellow canaries (Crithagra flaviventris), named canary chaphamaparvovirus 1 and -2 (CaChPV1 and CaChPV2), canary dependoparvovirus 1 and -2 (CaDePV1 and CaDePV2). The whole genome sequences of CaChPV1, CaChPV2, CaDePV1, and CaD...
Source: Virology - April 10, 2024 Category: Virology Authors: Yuhao Zhang Saranika Talukder Md Safiul Alam Bhuiyan Lei He Subir Sarker Source Type: research
Porcine respiratory coronavirus genome sequences; comparisons and relationships to transmissible gastroenteritis viruses
In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences. The whole genome sequences of Danish (1/90-DK), Italian (PRCV15087/12 III NPTV Parma), and Belgian PRCV (91V44) strains are presented. These sequences were aligned with nine other PRCV sequences from Europe and the US, and 43 TGEV sequences. Following alignment of the PRCV sequences, it was apparent that multiple amino acid variations in the structural proteins were distinct between the European and US strains. The alignments were used to build phyloge...
Source: Virology - April 10, 2024 Category: Virology Authors: Amalie Ehlers Bedsted Thomas Bruun Rasmussen Laura D Martinenghi Anette B øtner Hans Nauwynck Graham J Belsham Source Type: research
Opportunistic sampling of yellow canary (Crithagra flaviventris) has revealed a high genetic diversity of detected parvoviral sequences
Virology. 2024 Apr 4;595:110081. doi: 10.1016/j.virol.2024.110081. Online ahead of print.ABSTRACTParvoviruses are known to be significant viral pathogens that infect a wide range of species globally. However, little is known about the parvoviruses circulating in Australian birds, including yellow canaries. Here, we present four parvoviral sequences including three novel parvoviruses detected from 10 yellow canaries (Crithagra flaviventris), named canary chaphamaparvovirus 1 and -2 (CaChPV1 and CaChPV2), canary dependoparvovirus 1 and -2 (CaDePV1 and CaDePV2). The whole genome sequences of CaChPV1, CaChPV2, CaDePV1, and CaD...
Source: Virology - April 10, 2024 Category: Virology Authors: Yuhao Zhang Saranika Talukder Md Safiul Alam Bhuiyan Lei He Subir Sarker Source Type: research
Porcine respiratory coronavirus genome sequences; comparisons and relationships to transmissible gastroenteritis viruses
In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences. The whole genome sequences of Danish (1/90-DK), Italian (PRCV15087/12 III NPTV Parma), and Belgian PRCV (91V44) strains are presented. These sequences were aligned with nine other PRCV sequences from Europe and the US, and 43 TGEV sequences. Following alignment of the PRCV sequences, it was apparent that multiple amino acid variations in the structural proteins were distinct between the European and US strains. The alignments were used to build phyloge...
Source: Virology - April 10, 2024 Category: Virology Authors: Amalie Ehlers Bedsted Thomas Bruun Rasmussen Laura D Martinenghi Anette B øtner Hans Nauwynck Graham J Belsham Source Type: research
Opportunistic sampling of yellow canary (Crithagra flaviventris) has revealed a high genetic diversity of detected parvoviral sequences
Virology. 2024 Apr 4;595:110081. doi: 10.1016/j.virol.2024.110081. Online ahead of print.ABSTRACTParvoviruses are known to be significant viral pathogens that infect a wide range of species globally. However, little is known about the parvoviruses circulating in Australian birds, including yellow canaries. Here, we present four parvoviral sequences including three novel parvoviruses detected from 10 yellow canaries (Crithagra flaviventris), named canary chaphamaparvovirus 1 and -2 (CaChPV1 and CaChPV2), canary dependoparvovirus 1 and -2 (CaDePV1 and CaDePV2). The whole genome sequences of CaChPV1, CaChPV2, CaDePV1, and CaD...
Source: Virology - April 10, 2024 Category: Virology Authors: Yuhao Zhang Saranika Talukder Md Safiul Alam Bhuiyan Lei He Subir Sarker Source Type: research
Porcine respiratory coronavirus genome sequences; comparisons and relationships to transmissible gastroenteritis viruses
In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences. The whole genome sequences of Danish (1/90-DK), Italian (PRCV15087/12 III NPTV Parma), and Belgian PRCV (91V44) strains are presented. These sequences were aligned with nine other PRCV sequences from Europe and the US, and 43 TGEV sequences. Following alignment of the PRCV sequences, it was apparent that multiple amino acid variations in the structural proteins were distinct between the European and US strains. The alignments were used to build phyloge...
Source: Virology - April 10, 2024 Category: Virology Authors: Amalie Ehlers Bedsted Thomas Bruun Rasmussen Laura D Martinenghi Anette B øtner Hans Nauwynck Graham J Belsham Source Type: research
