World Journal of Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Efficient gene editing in the slow-growing, non-sporulating, melanized, endophytic fungus Berkleasmium sp. Dzf12 using a CRISPR/Cas9 system
AbstractThe endophytic fungusBerkleasmium sp. Dzf12 that was isolated fromDioscorea zingiberensis, is a proficient producer of palmarumycins, which are intriguing polyketides of the spirobisnaphthalene class. These compounds displayed a wide range of bioactivities, including antibacterial, antifungal, and cytotoxic activities. However, conventional genetic manipulation ofBerkleasmium sp. Dzf12 is difficult and inefficient, partially due to the slow-growing, non-sporulating, and highly pigmented behavior of this fungus. Herein, we developed a CRISPR/Cas9 system suitable for gene editing inBerkleasmium sp. Dzf12. The protopl...
Source: World Journal of Microbiology and Biotechnology - April 23, 2024 Category: Microbiology Source Type: research

Evaluation of multi-heavy metal tolerance traits of soil-borne fungi for simultaneous removal of hazardous metals
The objective of the present study was to isolate the fungi from the agricultural soil of Kashmir valley, investigate their multi-metal tolerance to heavy metals and evaluate the metal uptake capacities of the resistant fungi. The fungi were isolated and identified on the basis of morphological and molecular approach (ITS1 and ITS4). The tolerance limits of the isolated fungal strains to various doses of lead (Pb), cadmium (Cd), zinc (Zn), chromium (Cr), copper (Cu), nickel (Ni), and cobalt (Co) was evaluated. Five fungal strains,Aspergillus niger, Fusarium oxysporum, Fusarium verticillioides,Aspergillus fischeri, Epicoccu...
Source: World Journal of Microbiology and Biotechnology - April 22, 2024 Category: Microbiology Source Type: research

Bacterial extracellular vesicles: biotechnological perspective for enhanced productivity
AbstractBacterial extracellular vesicles (BEVs) are non-replicative nanostructures released by Gram-negative and Gram-positive bacteria as a survival mechanism and inter- and intraspecific communication mechanism. Due to BEVs physical, biochemical, and biofunctional characteristics, there is interest in producing and using them in developing new therapeutics, vaccines, or delivery systems. However, BEV release is typically low, limiting their application. Here, we provide a biotechnological perspective to enhance BEV production, highlighting current strategies. The strategies include the production of hypervesiculating str...
Source: World Journal of Microbiology and Biotechnology - April 20, 2024 Category: Microbiology Source Type: research

Growth-dependent cr(VI) reduction by Alteromonas sp. ORB2 under haloalkaline conditions: toxicity, removal mechanism and effect of heavy metals
This study investigated Cr(VI) reduction under complex haloalkaline conditions by anAlteromonas sp. ORB2 isolated from aerobic granular sludge cultivated from the seawater-microbiome. Optimum growth ofAlteromonas sp. ORB2 was observed under haloalkaline conditions at 3.5 –9.5% NaCl and pH 7–11. The bacterial growth in normal culture conditions (3.5% NaCl; pH 7.6) was not inhibited by 100 mg/l Cr(VI)/ As(V)/ Pb(II), 50 mg/l Cu(II) or 5 mg/l Cd(II). Near complete reduction of 100 mg/l Cr(VI) was achieved within 24 h at 3.5–7.5% NaCl and pH 8–11. Cr(VI) re duction byAlteromonassp. ORB2 was not inhibited by 100  ...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Metagenomic investigations into the microbial consortia, degradation pathways, and enzyme systems involved in the biodegradation of plastics in a tropical lentic pond sediment
AbstractThe exploitation of exciting features of plastics for diverse applications has resulted in significant plastic waste generation, which negatively impacts environmental compartments, metabolic processes, and the well-being of aquatic ecosystems biota. A shotgun metagenomic approach was deployed to investigate the microbial consortia, degradation pathways, and enzyme systems involved in the degradation of plastics in a tropical lentic pond sediment (APS). Functional annotation of the APS proteome (ORFs) using the PlasticDB database revealed annotation of 1015 proteins of enzymes such as depolymerase, esterase, lipase...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Co-production of metallo- β-lactamase and OXA-type β-lactamases in carbapenem-resistant Acinetobacter baumannii clinical isolates in North East India
This study aimed to investigate the antibiotic resistance pattern of carbapenem-resistantA. baumannii isolates collected from different clinical settings in North East India, focusing on their genotypic and phenotypic resistance profiles. A total of 172 multidrug-resistantA. baumannii isolates were collected and subjected to antibiotic susceptibility test using the Kirby –Bauer disk diffusion method. Various phenotypic tests were performed to detect extended-spectrum β-lactamase (ESBL), metallo-β-lactamase (MBL), class C AmpC β-lactamase (AmpC), and carbapenem hydrolyzing class D β-lactamase (CHDL) production among t...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

How does active yeast supplementation reduce the deleterious effects of aflatoxins in Wistar rats? A radiolabeled assay and histopathological study
AbstractThe aim of this study was to investigate the mechanisms by which yeasts (Saccharomyces cerevisiae) control the toxic effects of aflatoxins, which are not yet fully understood. Radiolabeled aflatoxin B1 (AFB13H) was administered by gavage to Wistar rats fed with aflatoxin (AflDiet) and aflatoxin supplemented with active dehydrated yeast Y904 (AflDiet  + Yeast). The distribution of AFB13H and its metabolites were analyzed at 24, 48 and 72  h by tracking back of the radioactivity. No significant differences were observed between the AflDiet and AflDiet + Yeast groups in terms of the distribution of labeled af...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Anti-obesity potentiality of Lactiplantibacillus plantarum E2_MCCKT isolated from a fermented beverage, haria: a high fat diet-induced obese mice model study
In conclusion, the probioticLp. plantarum E2_MCCKT might have significant anti-obesity effects on mice. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Linking the protease activity to the nematicidal action of edible mushroom
AbstractBiological control using edible mushrooms as natural enemies is a sustainable alternative for pest management. Despite the well-established literature on toxins and secondary metabolites produced by these fungi in the biochemical control of nematodes, the nematicidal activity of proteases from differentPleurotus species is yet to be investigated. Therefore, this study aimed to correlate protease to the nematicidal activity of different mushrooms,Pleurotus sp.,P. ostreatus (SB),P. ostreatus (Pearl), andP. djamor. For such a purpose, we performed motility assays ofPanagrellus sp. at different time intervals, 6, 12, a...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Characterization of anthranilic acid produced by Virgibacillus salarius MML1918 and its bio-imaging application
In this study, we utilized metabolic engineering approaches to produce anthranilic acid from the halophilic bacteriumVirgibacillus salarius MML1918. The halophilic bacteria were grown in an optimized production medium, and mass production of secondary metabolites was made in ATCC medium 1097 Proteose peptone —for halophilic bacteria and subjected to column chromatography followed by sub-column chromatography the single band for the purified compound was confirmed. Further, various spectral analyses were made for the partially purified compounds, and fluorescence microscopy for fungal cell observation was performed. The p...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Research and application progress of microbial β-mannanases: a mini-review
AbstractMannan is a predominant constituent of cork hemicellulose and is widely distributed in various plant tissues. β-Mannanase is the principal mannan-degrading enzyme, which breaks down the β-1,4-linked mannosidic bonds in mannans in an endo-acting manner. Microorganisms are a valuable source of β-mannanase, which exhibits catalytic activity in a wide range of pH and temperature, making it highly versatile a nd applicable in pharmaceuticals, feed, paper pulping, biorefinery, and other industries. Here, the origin, classification, enzymatic properties, molecular modification, immobilization, and practical application...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Functional expression, purification, biochemical and biophysical characterizations, and molecular dynamics simulation of a histidine acid phosphatase from Saccharomyces cerevisiae
AbstractA histidine acid phosphatase (HAP) (PhySc) with 99.50% protein sequence similarity with PHO5 fromSaccharomyces cerevisiae was expressed functionally with the molecular mass of ∼110 kDa through co-expression along with the set of molecular chaperones dnaK, dnaJ, GroESL. The purified HAP illustrated the optimum activity of 28.75 ± 0.39 U/mg at pH 5.5 and 40 ˚C. The Km and Kcat values towards calcium phytate were 0.608  ± 0.09 mM and 650.89 ± 3.6 s− 1. The half-lives (T1/2) at 55 and 60 ˚C were 2.75 min and 55 s, respectively. The circular dichroism (CD) demonstrated that PhySc includes 30.5...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Integrating bioprocess and metagenomics studies to enhance humic acid production from rice straw
This study aimed to examine the influence of a microbial consortium and other additives (chicken manure, urea, olive mill waste, zeolite, and biochar) on the composting process of rice straw and the subsequent production of humic acid. Results showed that among the fungal species,Thermoascus aurantiacus exhibited the most prominent impact in expediting maturation and improving compost quality, andBacillus subtilis was the most abundant bacterial species based on metagenomics analysis. The highest temperature, C/N ratio reduction, and amount of humic acid production (Respectively in lab 61  °C, 54.67%, 298 g kg−1 and i...
Source: World Journal of Microbiology and Biotechnology - April 17, 2024 Category: Microbiology Source Type: research

Recent advances in the use of Trichoderma-containing multicomponent microbial inoculants for pathogen control and plant growth promotion
AbstractChemical pesticides and fertilizers are used in agricultural production worldwide to prevent damage from plant pathogenic microorganisms, insects, and nematodes, to minimize crop losses and to preserve crop quality. However, the use of chemical pesticides and fertilizers can severely pollute soil, water, and air, posing risks to the environment and human health. Consequently, developing new, alternative, environment-friendly microbial soil treatment interventions for plant protection and crop yield increase has become indispensable. Members of the filamentous fungal genusTrichoderma (Ascomycota, Sordariomycetes, Hy...
Source: World Journal of Microbiology and Biotechnology - April 13, 2024 Category: Microbiology Source Type: research

Influence of flavonoids from Sedum aizoon L. on mitochondrial function of Rhizopus nigricans in strawberry
In this study, the minimum inhibitory concentration (MIC) of FSAL onR. nigricans and cell apoptosis tests were studied to explore the inhibitory effect of FSAL onR. nigricans. The effects of FSAL on mitochondria ofR. nigricans were investigated through the changes of mitochondrial permeability transition pore(mPTP), mitochondrial membrane potential(MMP), Ca2+ content, H2O2 content, cytochromec (Cyt c) content, the related enzyme activity and related genes of mitochondria. The results showed that the MIC of FSAL onR. nigricans was 1.800  mg/mL, with the addition of FSAL (1.800 mg/mL), the mPTP openness ofR. nigricans incr...
Source: World Journal of Microbiology and Biotechnology - April 13, 2024 Category: Microbiology Source Type: research