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Insulin-stimulated phosphorylation of endothelial nitric oxide synthase at Ser615 contributes to nitric oxide synthesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Insulin stimulates endothelial nitric oxide synthesis via protein kinase B/Akt-mediated phosphorylation and activation of endothelial nitric oxide synthase at Ser1177. In previous studies, we have demonstrated that stimulation of endothelial nitric oxide synthase phosphorylation at Ser1177 may be required, yet is not sufficient for insulin-stimulated nitric oxide synthesis. We therefore investigated the role of phosphorylation of endothelial nitric oxide synthase at alternative sites to Ser1177 as candidate parallel mechanisms contributing to insulin-stimulated nitric oxide synthesis. Stimulation of human aortic endothelia...
Source: BJ Signal - November 20, 2009 Category: Biochemistry Authors: S A Ritchie, C F Kohlhaas, A R Boyd, K C Yalla, K Walsh, J MC Connell, I P Salt Tags: BJ Signal Source Type: journals

Effects of Adenovirus-Mediated Delivery of the Human Hepatocyte Growth Factor Gene in Experimental Radiation-Induced Heart DiseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Based on the study findings, hepatocyte growth factor gene transfer can attenuate radiation-induced cardiac injury and can preserve cardiac function. (Source: International Journal of Radiation Oncology * Biology * Physics)
Source: International Journal of Radiation Oncology * Biology * Physics - November 19, 2009 Category: Radiology Authors: Shunying Hu, Yundai Chen, Libing Li, Jinlong Chen, Bin Wu, Xiao Zhou, Guang Zhi, Qingfang Li, Rongliang Wang, Haifeng Duan, Zikuan Guo, Yuefeng Yang, Fengjun Xiao, Hua Wang, Lisheng Wang Tags: Biology Contributions Source Type: journals

Effects of angiopoietin-1 on attachment and metastasis of human gastric cancer cell line BGC-823.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: Transfection of Ang-1 into human gastric cancer cell line BGC-823 can significantly increase expression of integrin beta1 and CD44V6, by which cell adhesion and metastasis to the ECM are promoted. PMID: 19916173 [PubMed - in process] (Source: World Journal of Gastroenterology : WJG)
Source: World Journal of Gastroenterology : WJG - November 19, 2009 Category: Gastroenterology Authors: Ou XL, Chen HJ, Sun WH, Hang C, Yang L, Guan YY, Yan F, Chen BA Tags: World J Gastroenterol Source Type: journals

HIV-1 STEP Vaccine Trial Failure Blamed on Adenovirus ShellEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The increase in HIV infections among vaccines, leading to a halt of the HIV type 1 STEP vaccine trial, has been traced to the adenovirus shell. Medscape Medical News (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - November 18, 2009 Category: Consumer Health News Tags: HIV/AIDS Source Type: news

Scientists Learn Why Failed HIV Vaccine Actually Increased the Risk of InfectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
UK and US researchers think they may have discovered why a recently studied adenovirus-based vaccine against HIV-1 actually increased the risk of infection in some individuals. Reuters Health Information (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - November 18, 2009 Category: Consumer Health News Tags: HIV/AIDS Source Type: news

Adeno-associated viral vectors and their redirection to cell-type specific receptors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Efficient and specific delivery of genes to the cell type of interest is a crucial issue in gene therapy. Adeno-associated virus (AAV) has gained particular interest as gene vector recently and is therefore the focus of this chapter. Its low frequency of random integration into the genome and the moderate immune response make AAV an attractive platform for vector design. Like in most other vector systems, the tropism of AAV vectors limits their utility for certain tissues especially upon systemic application. This may in part be circumvented by using AAV serotypes with an in vivo gene transduction pattern most closely ...
Source: Advances in Genetics - November 18, 2009 Category: Genetics & Stem Cells Authors: Michelfelder S, Trepel M Tags: Adv Genet Source Type: journals

Conditionally replicating adenovirus improves gene replication efficiency and anticancer effect of E1-deleted adenovirus carrying TRAIL in head and neck squamous cell carcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, this combined strategy using CRAd and adenovirus carrying a therapeutic gene increased the gene transfer rate and enhanced antitumor effects. We expect that this combination strategy could be extended to a multitarget cancer gene therapy by combining multiple adenoviruses and CRAd. (Cancer Sci 2009) (Source: Cancer Science)
Source: Cancer Science - November 18, 2009 Category: Cancer & Oncology Authors: Seon-Hui Shim, Choon-Taek Lee, J. Hun Hah, Jae-Jung Lee, Seok-Woo Park, Dae Seog Heo, Myung-Whun Sung Source Type: journals

Effective relief of neuropathic pain by adeno-associated virus-mediated expression of a small hairpin RNA against GTP cyclohydrolase 1Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Therefore, the data suggests that GCH1 levels can be reduced by introducing rAAV-shGCH1, leading to pain relief. Based on the results, we propose that GCH1 modulation may be developed as a clinically applicable gene therapy strategy to treat neuropathic pain. (Source: Molecular Pain)
Source: Molecular Pain - November 18, 2009 Category: Molecular Biology Authors: Sung Jin KimWon Il LeeYoon Sun LeeDong Hou KimJin Woo ChangSeong Who KimHeuiran Lee Source Type: journals

Caveolin gene transfer improves glucose metabolism in diabetic mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our results suggest that caveolin is an important regulator of glucose metabolism that can enhance insulin signals. PMID: 19923424 [PubMed - as supplied by publisher] (Source: American Journal of Physiology. Cell Physiology)
Source: American Journal of Physiology. Cell Physiology - November 18, 2009 Category: Cytology Authors: Otsu K, Toya Y, Oshikawa J, Kurotani R, Yazawa T, Sato M, Yokoyama U, Umemura S, Minamisawa S, Okumura S, Ishikawa Y Tags: Am J Physiol Cell Physiol Source Type: journals

The effect of endostatin mediated by human mesenchymal stem cells on ovarian cancer cells in vitroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion We found that MSCs possessed great migratory capacity in vitro and the human ovarian adenocarcinoma cell line SKOV3 could significantly induce the migration of MSCs. Our results provided evidence that MSCs could be utilized as a powerful delivery system of endostatin. The endostatin produced by MSC-EN cells could inhibit the proliferation of SKOV3 cells. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00432-009-0728-8Authors Jing Jiang, The Second Affiliated Hospital of Harbin Medical University Department of Obstetrics and Gynecology No. 246, Xue Fu Road, Nan Gang District ...
Source: Journal of Cancer Research and Clinical Oncology - November 17, 2009 Category: Cancer & Oncology Tags: Journal of Cancer Research and Clinical Oncology Source Type: journals

Adenovirus E3 MHC inhibitory genes but not TNF/Fas apoptotic inhibitory genes expressed in {beta} cells prevent autoimmune diabetes [Immunology]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To mimic events and molecules involved in type 1 insulin-dependent diabetes mellitus (T1D), we previously designed a transgenic (tg) mouse... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - November 17, 2009 Category: Science Authors: Horwitz, M. S., Efrat, S., Christen, U., von Herrath, M. G., Oldstone, M. B. A. Tags: Immunology Source Type: journals

HIV vaccine failure probably caused by virus used, says new researchEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Immunity to adenovirus means some people had a higher risk of HIV infection in STEP Trial - News Release (Source: Imperial College News)
Source: Imperial College News - November 17, 2009 Category: Biomedical Science Source Type: organizations

Safety and tolerability of putaminal AADC gene therapy for Parkinson diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study provides class IV evidence that bilateral intrastriatal infusion of adeno-associated viral type 2 vector containing the human AADC gene improves mean scores on the Unified Parkinson’s Disease Rating Scale by approximately 30% in the on and off states, but the surgical procedure may be associated with an increased risk of intracranial hemorrhage and self-limited headache. (Source: Neurology)
Source: Neurology - November 16, 2009 Category: Neurology Authors: Christine, C. W., Starr, P. A., Larson, P. S., Eberling, J. L., Jagust, W. J., Hawkins, R. A., VanBrocklin, H. F., Wright, J. F., Bankiewicz, K. S., Aminoff, M. J. Tags: PET, Parkinson's disease/Parkinsonism, Gene therapy ARTICLES Source Type: journals

Amelioration of myocarditis by HVEM-overexpressing dendritic cells through induction of IL-10-producing cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion Myosin-DC-AdHVEM cell gene therapy appears to be a safe and effective way of inhibiting the development of EAM. The signal induced by HVEM seems to play different roles in different cells. (Source: Cardiovascular Research)
Source: Cardiovascular Research - November 16, 2009 Category: Cardiology Authors: Cai, G., Wang, H., Qin, Q., Zhang, J., Zhu, Z., Liu, M., Shen, Q. Tags: ORIGINAL ARTICLES Source Type: journals

New Adenovirus in Bats, Germany, M. Sonntag et al.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - November 16, 2009 Category: Infectious Diseases Source Type: journals

The 5'-end heterogeneity of adenovirus virus-associated RNAI contributes to the asymmetric guide strand incorporation into the RNA-induced silencing complexEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human Adenovirus type 5 encodes two short RNA polymerase III transcripts, the virus-associated (VA) RNAI and VA RNAII, which can adopt stable hairpin structures that resemble micro-RNA precursors. The terminal stems of the VA RNAs are processed into small RNAs (mivaRNAs) that are incorporated into RISC. It has been reported that VA RNAI has two transcription initiation sites, which produce two VA RNAI species; a major species, VA RNAI(G), which accounts for 75% of the VA RNAI pool, and a minor species, VA RNAI(A), which initiates transcription three nucleotides upstream compared to VA RNAI(G). We show that this 5'-heteroge...
Source: Nucleic Acids Research - November 13, 2009 Category: Research Authors: Xu, N., Gkountela, S., Saeed, K., Akusjarvi, G. Tags: RNA Source Type: journals

Antiviral activity of a composition of Gentiana lutea L., Primula veris L., Sambucus nigra L., Rumex spec. and Verbena officinalis L. (Sinupret®) against viruses causing respiratory infectionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sinupret®, an aqueous-ethanolic extract from five plants, Gentiana lutea L., Primula veris L., Sambucus nigra L., Rumex spec. and Verbena officinalis L., is frequently used in the treatment of acute and chronic rhinosinusitis and respiratory viral infections such as common cold. To date little is known about basic mechanisms of its potential antiviral activity. Therefore experiments have been performed for the detection of its antiviral activity against a broad panel of human pathogenic enveloped and non-enveloped RNA and DNA viruses causing infections of the upper respiratory tract: influenza A virus, parainfluenza virus...
Source: European Journal of Integrative Medicine - November 13, 2009 Category: Complementary Medicine Authors: B. Glatthaar, A. Saalmüller, J. Haunschild, A. Amon Source Type: journals

LONG-TERM MATERNAL CARDIOVASCULAR FUNCTION IN A MOUSE MODEL OF sFlt-1-INDUCED PREECLAMPSIA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our results indicate, that in a mouse model over-expression of sFlt-1 does not lead to increased in BP and altered vascular function in the absence of the pregnancy and has no long-term effect on BP and vascular function in the postpartum mothers. Our findings favor the hypothesis, that increased CVD in women with history of preeclampsia are likely the result of preexisting risk factors common to preeclampsia and CVD. Key words: vascular reactivity, postpartum, blood pressure, adenovirus. PMID: 19915174 [PubMed - as supplied by publisher] (Source: American Journal of Physiology. Heart and Circulatory Physiology)
Source: American Journal of Physiology. Heart and Circulatory Physiology - November 13, 2009 Category: Physiology Authors: Bytautiene E, Lu F, Tamayo EH, Hankins GD, Longo M, Kublickiene K, Saade GR Tags: Am J Physiol Heart Circ Physiol Source Type: journals

Adenovirus vector targeted to dorsal root ganglia as a future tool for the treatment of sensory neuron diseasesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Future Neurology , November 2009, Vol. 4, No. 6, Pages 693-696. (Source: Future Neurology)
Source: Future Neurology - November 12, 2009 Category: Neurology Tags: article Source Type: journals

Inhibition of MAPK signaling by eNOS gene transfer improves ventricular remodeling after myocardial infarction through reduction of inflammation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) may play an important role in attenuating cardiac remodeling and apoptosis after myocardial infarction. However, the anti-inflammation effects of eNOS in infarcted myocardium and the role of MAPK signaling in eNOS/NO mediated cardiac remodeling have not yet been elucidated. Adenovirus carrying Human eNOS gene was delivered locally into heart 4 days prior to induction of myocardial infarction (MI) by left anterior descending coronary artery ligation. Monocyte/macrophage infiltration was detected by ED-1 immunohistochemistry. Western blot was employed to exam...
Source: Molecular Biology Reports - November 12, 2009 Category: Molecular Biology Authors: Chen LL, Zhu TB, Yin H, Huang J, Wang LS, Cao KJ, Yang ZJ Tags: Mol Biol Rep Source Type: journals

Induction of T cells suppression by dendritic cells transfected with VSIG4 recombinant adenovirus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
VSIG4 has been recently described as a B7 family-related protein. The immunotherapeutic potential of dendritic cells (DCs) transfected with VSIG4 recombinant adenovirus has not been characterized. In the present study, DCs were transfected with human VSIG4 (hVSIG4) recombinant adenovirus, a novel costimulatory molecule known to be a potent inhibitor of T cell activation. Transfected DCs were cocultured with allogeneic T cells and proliferation, cytokine production and T cell activation marker expression were assessed. The results showed that T cell proliferation potential, cytokine production and activation marker expr...
Source: Immunology Letters - November 12, 2009 Category: Allergy & Immunology Authors: Xu S, Sun Z, Wu X, Liu J, He J, Song D, Shan G, Liu H, Yang Z Tags: Immunol Lett Source Type: journals

Generation of Full-Length Functional Antibody against PreS2 of Hepatitis B Virus in Hepatic Cells In Vitro from Bicistrons Mediated by Gutless AdenovirusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: BioDrugs)
Source: BioDrugs - November 11, 2009 Category: Drugs & Pharmacology Authors: Yin, YuexiangLin, FangZhuang, QiangLiu, LiQian, Cheng Tags: Original Research Article Source Type: journals

Metformin decreases IGF-1-induced cell proliferation and protein synthesis through AMP-activated protein kinase in cultured bovine granulosa cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although its mechanism of action is still unclear, metformin is an anti-diabetic drug effective to restore cyclicity and spontaneous ovulation in women with polycystic ovary syndrome. It also may reduce the risk of cancer. We have recently shown that metformin treatment decreases steroidogenesis through AMPK (Adenosine Monophosphate activated kinase) in granulosa cells of various species. Here, we investigated the effects and the molecular mechanisms of metformin in IGF-1-induced proliferation and protein synthesis in cultured bovine granulosa cells. Treatment with metformin (10 mM) for 24h reduced cell proliferation a...
Source: Reproduction - November 11, 2009 Category: Reproduction Medicine Authors: Tosca L, Rame C, Chabrolle C, Tesseraud S, Dupont J Tags: Reproduction Source Type: journals

Targeting for insulin-like growth factor-I receptor with short hairpin RNA for human digestive/gastrointestinal cancersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions shIGF-IR may have therapeutic utility in human digestive/gastrointestinal cancers, both alone and in combination with chemotherapy. Content Type Journal ArticleCategory Original Article—Alimentary TractDOI 10.1007/s00535-009-0151-6Authors Yu Wang, Sapporo Medical University First Department of Internal Medicine S-1, W-16, Chuo-ku Sapporo 060-8543 JapanYasushi Adachi, Sapporo Medical University First Department of Internal Medicine S-1, W-16, Chuo-ku Sapporo 060-8543 JapanArisa Imsumran, Sapporo Medical University First Department of Internal Medicine S-1, W-16, Chuo-ku Sapporo 060-8543 Jap...
Source: Journal of Gastroenterology - November 9, 2009 Category: Gastroenterology Tags: Journal of Gastroenterology Source Type: journals

A new synthetic protein, TAT-RH, inhibits tumor growth through the regulation of NFkappaB activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our data suggest that GRK5-RH inhibition of NFkappaB is a novel and effective anti-tumoral strategy and TAT-RH could be an useful tool in the fighting of cancer. (Source: Molecular Cancer)
Source: Molecular Cancer - November 9, 2009 Category: Cancer & Oncology Authors: Daniela SorrientoAlfonso CampanileGaetano SantulliEleonora LeggieroLucio PastoreBruno TrimarcoGuido Iaccarino Source Type: journals

Chimeric adenoviral vectors incorporating a fiber of human adenovirus 3 efficiently mediate gene transfer into prostate cancer cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have developed a range of adenoviral (Ad) vectors based on human adenovirus serotype 5 (HAdV-5) displaying the fiber shaft and knob domains of species B viruses (HAdV-3, -11, or -35). These species B Ads utilize different cellular receptors than HAdV-5 for infection. We evaluated whether Ad vectors displaying species B fiber shaft and knob domains (Ad5F3Luc1, Ad5F11Luc1, and Ad5F35Luc1) would efficiently infect cancer cells of distinct origins, including prostate cancer.The fiber chimeric Ad vectors were genetically generated and compared with the original Ad vector (Ad5Luc1) for transductional efficiency in a variety o...
Source: The Prostate - November 9, 2009 Category: Urology & Nephrology Authors: Miho Murakami, Hideyo Ugai, Natalya Belousova, Alexander Pereboev, Paul Dent, Paul B. Fisher, Maaike Everts, David T. Curiel Source Type: journals

Corrigendum: Baseline Ad5 serostatus does not predict Ad5 HIV vaccine–induced expansion of adenovirus-specific CD4+ T cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Corrigendum: Baseline Ad5 serostatus does not predict Ad5 HIV vaccine–induced expansion of adenovirus-specific CD4+ T cells Nature Medicine 15, 1333 (2009). doi:10.1038/nm1109-1333b Authors: Natalie A Hutnick, Diane G Carnathan, Sheri A Dubey, George Makedonas, Kara S Cox, Lisa Kierstead, Sarah J Ratcliffe, Michael N Robertson, Danilo R Casimiro, Hildegund C J Ertl & Michael R Betts (Source: Nature Medicine)
Source: Nature Medicine - November 6, 2009 Category: Journals (General) Authors: Natalie A HutnickDiane G CarnathanSheri A DubeyGeorge MakedonasKara S CoxLisa KiersteadSarah J RatcliffeMichael N RobertsonDanilo R CasimiroHildegund C J ErtlMichael R Betts Tags: Corrigendum Source Type: journals

[Comment] Promises and challenges of genetic therapy for blindnessEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Patients with inherited congenital blindness (Leber's congenital amaurosis) have a severe handicap, even with modern support systems. In patients with Leber's congenital amaurosis due to mutations in the RPE65 gene, subretinal injections of adeno-associated viruses carrying a normal RPE65 cDNA (AAV–RPE65) have been found to be safe and led to moderate improvement of retinal function in seven of nine patients. These studies were in patients between 17 and 26 years of age. Infants with RPE65 mutations are severely affected from birth, but their vision in most cases further deteriorates when they are teenagers. (Source: LANCET)
Source: LANCET - November 6, 2009 Category: Journals (General) Authors: Frans PM Cremers, Rob WJ Collin Tags: Comment Source Type: journals

The adenoviral E1A oncoprotein activates the Smad7 promoter: requirement of a functional E-box.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, these results unravel a novel mechanism of how the AdV E1A oncoprotein induces a cellular inhibitor of TGF-beta signalling. PMID: 19885573 [PubMed - in process] (Source: International Journal of Oncology)
Source: International Journal of Oncology - November 5, 2009 Category: Cancer & Oncology Authors: Dole VS, Smola H, Dooley S, Said HM, Oldak M, Pfister HJ, Smola S Tags: Int J Oncol Source Type: journals

Cytotoxic T lymphocyte therapy with donor T cells prevents and treats adenovirus and Epstein-Barr virus infections after haploidentical and matched unrelated stem cell transplantationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Viral infection or reactivation remains a major cause of morbidity and mortality after allogeneic stem cell transplantation. We now show that infusions of single cytotoxic T lymphocyte (CTL) lines (5 x 106-1.35 x 108 cells/m2) with specificity for 2 commonly detected viruses, Epstein-Barr virus (EBV) and adenovirus, can be safely administered to pediatric transplantation recipients receiving partially human leukocyte antigen–matched and haploidentical stem cell grafts (n = 13), without inducing graft-versus-host disease. The EBV-specific component of the CTLs expanded in vivo and persisted for more than 12 weeks, but...
Source: Blood - November 5, 2009 Category: Hematology Authors: Leen, A. M., Christin, A., Myers, G. D., Liu, H., Cruz, C. R., Hanley, P. J., Kennedy-Nasser, A. A., Leung, K. S., Gee, A. P., Krance, R. A., Brenner, M. K., Heslop, H. E., Rooney, C. M., Bollard, C. M. Tags: Immunobiology, Transplantation, Clinical Trials and Observations Source Type: journals

beta-Arrestins modulate Adenovirus vector induced innate immune responses: differential regulation by beta-arrestin-1 and beta-arrestin-2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we examined the role of beta-arrestins in Ad5 vector-induced inflammatory responses. Our studies reveal that both beta-arrestins are capable of modulating Ad5-vector-induced inflammatory responses in vivo and in vitro. Importantly, our studies divulge another level of complexity to these responses, as our results demonstrate beta-Arr1 to be a positive regulator, and beta-Arr2 a negative regulator of Ad5 induced innate immune responses. These data may allow gene therapy biologists to more accurately study the mechanisms underlying Ad5 vector induced immune responses, and may also direct future efforts to modu...
Source: Virus Research - November 5, 2009 Category: Virology Authors: Seregin SS, Appledorn DM, Patial S, Bujold M, Nance W, Godbehere S, Parameswaran N, Amalfitano A Tags: Virus Res Source Type: journals

A convenient enzyme-linked immunosorbent assay for rapid screening of anti-adeno-associated virus neutralizing antibodiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion This ELISA method may be useful for rapid screening of anti-AAV-2 neutralizing antibodies in candidates for gene therapy. (Source: Annals of Clinical Biochemistry)
Source: Annals of Clinical Biochemistry - November 4, 2009 Category: Biochemistry Authors: Ito, T., Yamamoto, S., Hayashi, T., Kodera, M., Mizukami, H., Ozawa, K., Muramatsu, S.-i. Tags: Short Reports Source Type: journals

Innovative Vector Design: Cross-Packaged, Self-Complementary and Now Trans-Splicing AAV VectorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human Gene Therapy Nov 2009, Vol. 20, No. 11: 1224-1225. (Source: Human Gene Therapy)
Source: Human Gene Therapy - November 4, 2009 Category: Genetics & Stem Cells Tags: article Source Type: journals

Orphan nuclear receptor small heterodimer partner inhibits angiotensin II- stimulated PAI-1 expression in vascular smooth muscle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Angiotensin II is a major effector molecule in the development of cardiovascular disease. In vascular smooth muscle cells (VSMCs), angiotensin II promotes cellular proliferation and extracellular matrix accumulation through the upregulation of plasminogen activator inhibitor-1 (PAI-1) expression. Previously, we demonstrated that small heterodimer partner (SHP) represses PAI-1 expression in the liver through the inhibition of Transforming growth factor-b (TGF-b) signaling pathways. Here, we investigated whether SHP inhibited angiotensin II-stimulated PAI-1 expression in VSMCs. Adenovirus-mediated overexpression of SHP (...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Lee KM, Seo HY, Kim MK, Min AK, Ryu SY, Kim YN, Park YJ, Choi HS, Lee KU, Park WJ, Park KG, Lee IK Tags: Exp Mol Med Source Type: journals

Inducible heat shock protein 70 enhances HPV31 viral genome replication and virion production during the differentiation-dependent life cycle in human keratinocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we carry out analyses in the natural host epithelial tissues to assess the role of inducible HSP70 (HSP70i) in the HPV infectious life cycle. For these studies we used the organotypic (raft) culture system to recapitulate the full viral life cycle of the high-risk HPV31. Upon heat shock of HPV31 infected organotypic tissues, we find high and sustained expression of HSP70i coincident with enhanced HPV genome replication and virion production. Whereas there is no clear effect on L1 expression levels, we find HSP70i and L1 interact and HSP70i colocalizes with and enhances the nuclear localization of L1 in differ...
Source: Virus Research - November 4, 2009 Category: Virology Authors: Song H, Moseley PL, Lowe SL, Ozbun MA Tags: Virus Res Source Type: journals

Platelet Derived Growth Factor B and Epithelial Mesenchymal Transition of Peritoneal Mesothelial Cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Platelet derived growth factor (PDGF) is involved in wound healing in various organ systems. Its potential role in the context of peritoneal injury following long term peritoneal dialysis is unclear. We used an adenovirus expressing the B chain of PDGF (AdPDGF-B) to assess its effect on pro-fibrotic pathways in the peritoneal membrane. To assess the transforming growth factor (TGF) beta independent effects of PDGF, we over-expressed PDGF-B in the peritoneum of either wild-type mice (Smad3(+/+)) or those with a deletion of the TGFbeta signaling protein Smad3 (Smad3(-/-)). PDGF-B induced sustained angiogenesis in both Sm...
Source: Matrix Biology - November 4, 2009 Category: Molecular Biology Authors: Patel P, West-Mays J, Kolb M, Rodrigues JC, Hoff CM, Margetts PJ Tags: Matrix Biol Source Type: journals

Syndecan-1 is the primary heparan sulfate proteoglycan mediating hepatic clearance of triglyceride-rich lipoproteins in miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we found by RT-PCR analysis that mouse hepatocytes expressed the membrane proteoglycans syndecan-1, -2, and -4 and glypican-1 and -4. Analysis of available proteoglycan-deficient mice showed that only syndecan-1 mutants (Sdc1–/– mice) accumulated plasma triglycerides. Sdc1–/– mice also exhibited prolonged circulation of injected human VLDL and intestinally derived chylomicrons. We found that mice lacking both syndecan-1 and hepatocyte heparan sulfate did not display accentuated triglyceride accumulation compared with single mutants, suggesting that syndecan-1 is the primary HS...
Source: Journal of Clinical Investigation - November 3, 2009 Category: Biomedical Science Authors: Kristin I. Stanford, Joseph R. Bishop, Erin M. Foley, Jon C. Gonzales, Ingrid R. Niesman, Joseph L. Witztum, Jeffrey D. Esko Source Type: journals

Small vessel vasculitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The pediatric small vessel vasculitides reviewed in this article are Henoch–Schönlein purpura (HSP) and the anti-neutrophil cytoplasmic antibody-associated vasculitides (AAV). The new classification criteria for HSP and Wegener’s granulomatosis are now validated and will facilitate the conduct of future epidemiological studies and clinical trials. The clinical manifestations of small vessel vasculitis in children are described, and current therapies discussed. There is a lack of good clinical trial data on which to base therapy for HSP. Similarly, data based on randomized controlled trials (RCT...
Source: Pediatric Nephrology - November 3, 2009 Category: Urology & Nephrology Tags: Pediatric Nephrology Source Type: journals

Decreased Expression of A-Kinase Anchoring Protein 150 in GT1 Neurons Decreases Neuron Excitability and Frequency of Intrinsic Gonadotropin-Releasing Hormone Pulses.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The frequency of intrinsic pulsatile GnRH secretion from endogenous GnRH neurons and GT1 GnRH cell lines is stimulated by increased intracellular cAMP levels. The downstream molecules comprising the cAMP signaling pathway are organized in microdomains by a family of scaffolding proteins, A-kinase anchoring proteins (AKAPs). These molecules tether protein kinase A, cAMP-specific phosphodiesterases, phosphatases to known substrates. In neurons AKAP150 organizes many of the signaling molecules known to regulate the excitability and intrinsic pulsatile activity of GnRH neurons. AKAP150 was expressed in both the GT1-1 and G...
Source: Endocrinology - November 3, 2009 Category: Endocrinology Authors: Chen Q, Weiner RI, Blackman BE Tags: Endocrinology Source Type: journals

Green Tea Polyphenol Epigallocatechin Gallate Reduces Endothelin-1 Expression and Secretion in Vascular Endothelial Cells: Roles for AMP-Activated Protein Kinase, Akt, and FOXO1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Epigallocatechin gallate (EGCG), a green tea polyphenol, promotes vasodilation by phosphatidylinositol 3-kinase-dependent activation of Akt and endothelial nitric oxide synthase to stimulate production of nitric oxide. Reduction in endothelin-1 (ET-1) synthesis may also increase bioavailability of nitric oxide. We hypothesized that the phosphatidylinositol 3-kinase-dependent transcription factor FOXO1 may mediate effects of EGCG to regulate expression of ET-1 in endothelial cells. EGCG treatment (10 muM, 8 h) of human aortic endothelial cells reduced expression of ET-1 mRNA, protein, and ET-1 secretion. We identified a...
Source: Endocrinology - November 3, 2009 Category: Endocrinology Authors: Reiter CE, Kim JA, Quon MJ Tags: Endocrinology Source Type: journals

The In Vitro and In Vivo Evaluation of ddC as a Topical Antiviral for Ocular Adenovirus Infections [Immunology and Microbiology]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
2',3'-Dideoxycytidine (ddC) represents a new candidate antiviral for the treatment of adenoviral ocular infections. The goals of the present study were to evaluate the in vitro and in vivo efficacy of ddC against adenovirus. (Source: Investigative Ophthalmology)
Source: Investigative Ophthalmology - November 2, 2009 Category: Opthalmology Authors: Romanowski, E. G., Yates, K. A., Gordon, Y. J. Tags: Immunology and Microbiology Source Type: journals

Plasmin plays a key role in the regulation of profibrogenic molecules in hepatic stellate cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Plasmin role in transforming growth factor-[beta] (TGF-[beta])-responsive gene regulation remains to be elucidated. Also, plasmin action on co-repressor Ski-related novel protein N (SnoN) and differential activation of matrix metalloproteinases (MMPs) are unknown. Thus, the role of plasmin on profibrogenic molecule expression, SnoN transcriptional kinetics and gelatinase activation was investigated. Hepatic stellate cells (HSC) were transduced with adenovirus-mediated human urokinase plasminogen activator (Ad-huPA) (4 × 109 viral particles/ml). Overexpression of urokinase plasminogen activator and therefore of plasmin, wa...
Source: Liver International - November 2, 2009 Category: Gastroenterology Authors: Abril Martínez-Rizo, Miriam Bueno-Topete, Jaime González-Cuevas, Juan Armendáriz-Borunda Source Type: journals

Inhibition of focal adhesion kinase and src increases detachment and apoptosis in human neuroblastoma cell linesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Neuroblastoma is the most common extracranial solid tumor of childhood. Focal adhesion kinase (FAK) is an intracellular kinase that is overexpressed in a number of human tumors including neuroblastoma, and regulates both cellular adhesion and survival. We have studied the effects of FAK inhibition upon neuroblastoma using adenovirus-containing FAK-CD (AdFAK-CD). Utilizing an isogenic MYCN+/MYCN- neuroblastoma cell line, we found that the MYCN+ cells are more sensitive to FAK inhibition with AdFAK-CD than their MYCN negative counterparts. In addition, we have shown that phosphorylation of Src is increased in the untreated i...
Source: Molecular Carcinogenesis - November 2, 2009 Category: Molecular Biology Authors: Elizabeth A. Beierle, Xiaojie Ma, Angelica Trujillo, Elena V. Kurenova, William G. Cance, Vita M. Golubovskaya Source Type: journals

Protective role of COMP-Ang1 in ischemic rat brainEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In cerebral ischemia, the induction of angiogenesis may represent a natural defense mechanism that enables the hypoxic brain to avoid progression into infarction. Angiopoietin-1 (Ang1) is known to produce non-leaky and stable blood vessel formation mainly by the Tie2 receptor. Therefore, we envisioned that the application of cartilage oligomeric matrix protein-Ang1 (COMP-Ang1), a soluble, stable, and potent form of Ang1, would promote angiogenesis and provide a protective effect following unilateral middle cerebral artery occlusion (MCAO) in rats. To this end, we employed a 2-hour-MCAO model, and treated rats with adenovir...
Source: Journal of Neuroscience Research - November 2, 2009 Category: Neuroscience Authors: Hye Young Shin, Yoon Jin Lee, Hak Joo Kim, Chul-Kee Park, Jong Hoon Kim, Kyu Chang Wang, Dong Gyu Kim, Gou Young Koh, Sun Ha Paek Source Type: journals

Otitis media: viruses, bacteria, biofilms and vaccines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Otitis media typically presents as either acute otitis media (AOM), with symptoms including fever, otalgia, otorrhoea or irritability and short duration; or as otitis media with effusion (OME), which is often asymptomatic and characterised by accumulation of fluid in the middle ear. Diagnostic certainty of otitis media is challenging, given the young age of patients and variability of symptoms. Otitis media predominantly occurs as coincident to viral upper respiratory tract infections and/or bacterial infections. Common viruses that cause upper respiratory tract infection are frequently associated with AOM and new-onse...
Source: Med J Aust - November 2, 2009 Category: Journals (General) Authors: Massa HM, Cripps AW, Lehmann D Tags: Med J Aust Source Type: journals

Enriching the viral-host interactomes with interactions mediated by SH3 domains.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Protein-protein interactions play an essential role in the regulation of most cellular processes. The process of viral infection is no exception and many viral pathogenic strategies involve targeting and perturbing host-protein interactions. The characterization of the host protein subnetworks disturbed by invading viruses is a major goal of viral research and may contribute to reveal fundamental biological mechanisms and to identify new therapeutic strategies. To assist in this approach, we have developed a database, VirusMINT, which stores in a structured format most of the published interactions between viral and ho...
Source: Amino Acids - November 2, 2009 Category: Biochemistry Authors: Carducci M, Licata L, Peluso D, Castagnoli L, Cesareni G Tags: Amino Acids Source Type: journals

The environment, geoepidemiology and ANCA-associated vasculitides.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Anti-neutrophil cytoplasmic antibodies, directed against constituents of granules of neutrophils and lysosomes of monocytes, are serological markers of small vessel vasculitides, including Wegener's granulomatosis, microscopic polyangiitis, Churg-Strauss syndrome and renal-limited vasculitis. These vasculitides are collectively termed ANCA-associated vasculitides (AAV). Environmental factors have been considered important in the development of ANCA, including silica, infection especially with Staphylococcus aureus, and drugs. Accelerated apoptosis of neutrophils induced through intratracheal instillation of silica may ...
Source: Autoimmunity Reviews - November 2, 2009 Category: Allergy & Immunology Authors: Chen M, Kallenberg CG Tags: Autoimmun Rev Source Type: journals

Viral gastroenteritisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The virology, immunology, diagnosis, clinical symptoms, treatment, epidemiology and prevention measures relating to the most common viral causes of acute gastroenteritis (rotaviruses, human caliciviruses, astroviruses and enteric adenoviruses) are briefly reviewed. Uncommon viral causes of acute gastroenteritis and viruses causing gastroenteritis in immunodeficient patients are mentioned. The main change over the past three years has been the development, licensing and wide application of new live attenuated rotavirus vaccines. (Source: Medicine)
Source: Medicine - November 1, 2009 Category: Internal Medicine Authors: Ulrich Desselberger, Jim Gray Tags: Gastrointestinal infections Source Type: journals

Clinical study of recombinant adenovirus-p53 combined with fractionated stereotactic radiotherapy for hepatocellular carcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion These results suggest that rAd-p53 combined with fSRT is a relatively safe and effective method for treating primary hepatocellular carcinoma compared with only fSRT. Thus, rAd-p53 combined with fractionated SRT may be preferred as a choice of local treatment for primary HCC when the patients are inoperable or when the patients refuse operation. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00432-009-0701-6Authors Zhi-xiang Yang, Third Military Medical University Cancer Center, Daping Hospital and Institute of Surgery Research 400042 Chongqing ChinaDong Wang, Third Militar...
Source: Journal of Cancer Research and Clinical Oncology - October 31, 2009 Category: Cancer & Oncology Tags: Journal of Cancer Research and Clinical Oncology Source Type: journals

Development of a quantitative PCR assay for the quantitation of bovine polyomavirus as a microbial source tracking tool.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, a quantitative PCR assay that is specific for bovine polyomaviruses was developed and used to determine the excretion level and concentration of bovine polyomaviruses in urine and environmental samples, including urban sewage, slaughterhouse sewage, and river water. A set of primers and a TaqMan probe were designed to target a 77-bp region of the bovine polyomavirus VP1 gene, and the conditions of the reaction were optimized. A detection limit was established at 1-10 genome copies per test tube. The assay was specific and produced negative results when samples containing human or porcine fecal contamination ...
Source: Journal of Virological Methods - October 31, 2009 Category: Virology Authors: Hundesa A, Bofill-Mas S, Motes CM, Rodriguez-Manzano J, Bach A, Casas M, Girones R Tags: J Virol Methods Source Type: journals