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Infectious Disease: Hepatitis B

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Total 12797 results found since Jan 2013.

Dissolving Microneedle Arrays as a Hepatitis B Vaccine Delivery System Adjuvanted by APC-Targeted Poly (Lactic-co-Glycolic Acid) (PLGA) Nanoparticles
This study verifies the feasibility of soluble composited microneedles administration in hepatitis B immunization, and provides new ideas for the development and application of non-injectable vaccine delivery systems.PMID:36697935 | DOI:10.1208/s12249-022-02473-9
Source: AAPS PharmSciTech - January 25, 2023 Category: Drugs & Pharmacology Authors: Xiaoling Zheng Jiahuan Zhu Caihong Zheng Zeng Tan Zhonghua Ji Jin Tao Yunchun Zhao Zhuwa Ji Ying Hu Source Type: research

Inhibitory effects of silver nanoparticles against adenovirus type 3 in vitro.
Abstract Adenoviruses are associated with respiratory, ocular, or gastrointestinal disease. With various species and high morbidity, adenoviruses are increasingly recognized as significant viral pathogen among pediatric and immunocompromised patients. However, there is almost no specific drug for treatment. Silver nanoparticles are demonstrated to be virucidal against influenza A (H1N1) virus, human immunodeficiency virus and Hepatitis B virus. Currently, there is no data regarding whether the silver nanoparticles inhibit the adenovirus or not. The aim of this study is to investigate the effect of silver nanoparti...
Source: Journal of Virological Methods - July 22, 2013 Category: Virology Authors: Chen N, Zheng Y, Yin J, Li X, Zheng C Tags: J Virol Methods Source Type: research

The enhanced immune response of hepatitis B virus DNA vaccine using SiO2@LDH nanoparticles as an adjuvant.
Abstract Various approaches have been used to improve systemic immune response to infectious disease or virus, and DNA vaccination has been demonstrated to be one of these effective ways to elicit protective immunity against pathogens. Our previous studies showed that layered double hydroxides (LDH) nanoparticles could be efficiently taken up by the MDDCs and had an adjuvant activity for DC maturation. To further enhance the immune adjuvant activity of LDH, core-shell structure SiO2@LDH nanoparticles were synthesized with an average diameter of about 210 nm. And its high transfection efficiency in vitro was demo...
Source: Biomaterials - October 4, 2013 Category: Materials Science Authors: Wang J, Zhu R, Gao B, Wu B, Li K, Sun X, Liu H, Wang S Tags: Biomaterials Source Type: research

Development and physicochemical, toxicity and immunogenicity assessments of recombinant hepatitis B surface antigen (rHBsAg) entrapped in chitosan and mannosylated chitosan nanoparticles: as a novel vaccine delivery system and adjuvant.
In this study chitosan nanoparticles (CS NPs) and mannosylated chitosan nanoparticles (MCH NPs) loaded with recombinant hepatitis B surface antigen (rHBsAg) was synthesized as a vaccine delivery system and assessed toxically and immunologically. The physicochemical properties of the nanoparticles (NPs) were determined by methods including scanning electron microscope (SEM) and dynamic light scattering (DLS). The morphology of the NPs was semi spherical and the average diameter of the loaded CS and MCH NPs was found to be 189 and 239 nm, respectively. The release studies showed that after the initial burst, both of the lo...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 22, 2017 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Electrophoresis-Assisted Multilayer Assembly of Nanoparticles for Sensitive Lateral Flow Immunoassay
Angew Chem Int Ed Engl. 2022 Nov 10. doi: 10.1002/anie.202215548. Online ahead of print.ABSTRACTLateral flow immunoassay (LFIA) is a rapid, simple, and inexpensive point-of-need method. A major limitation of LFIA is a high limit of detection (LOD), which impacts its diagnostic sensitivity. To overcome this limitation, we introduce a signal-enhancement procedure that is performed after completing LFIA and involves controllably moving biotin- and streptavidin-functionalized gold nanoparticles by electrophoresis. The nanoparticles link to immunocomplexes forming multilayer aggregates on the test strip, thus, enhancing the sig...
Source: Angewandte Chemie - November 10, 2022 Category: Chemistry Authors: Vasily G Panferov Nikita A Ivanov Tony Mazzulli Davor Brinc Vathany Kulasingam Sergey N Krylov Source Type: research

Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles.
Abstract A novel magnetic fluorescent encoded nanoimmunoassay system for multicomponent detection and separation of the subtrace pathogenic DNA (hepatitis B virus surface gene, HBV; hepatitis A virus poly the protein gene, HAV) was established based on new type of magnetic fluorescent encoded nanoparticles and sandwich immunoassay principle. This method combines multifunctional nanoparticles, immunoassay technique, fluorescence labeling, and magnetic separation of multicomponent technology. It has many advantages such as high sensitivity, low detection limit, easy operation, and great potential for development. Th...
Source: Biomed Res - February 18, 2016 Category: Research Authors: Liu Y, Zhang X, E Y, Fang F, Kuang G, Wang G Tags: Biomed Res Int Source Type: research

Viruses, Vol. 9, Pages 193: Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection
lundert Chronic infection with the hepatitis B virus (HBV) can lead to liver failure and can cause liver cirrhosis and hepatocellular carcinoma (HCC). Reliable means for detecting and monitoring HBV infection are essential to identify patients in need of therapy and to prevent HBV transmission. Nanomaterials with defined electrical, optical, and mechanical properties have been developed to detect and quantify viral antigens. In this review, we discuss the challenges in applying nanoparticles to HBV antigen detection and in realizing the bio-analytical potential of such nanoparticles. We discuss recent developments in g...
Source: Viruses - July 21, 2017 Category: Virology Authors: Maxim Shevtsov Lili Zhao Ulrike Protzer Maarten Klundert Tags: Review Source Type: research

Silica nanoparticles coated by poly(acrylic acid) brushes via host-guest interactions for detecting DNA sequence of Hepatitis B virus.
Abstract Poly(acrylic acid) (PAA) brushes coated onto silica nanoparticles have been widely utilized in bioassays due to their abilities of providing favorable microenvironments and ensuring good biological activities for biomolecules. However, traditional PAA brushes are synthesized by reversible addition-fragmentation chain transfer polymerization. Hence, it is generally difficult to control and characterize the molecular weight of the PAA brushes, which may depress the reproducibility and bring more uncertain results. Herein, atom transfer radical polymerization method is employed to synthesize β-cyclodextrin-...
Source: Talanta - February 12, 2018 Category: Chemistry Authors: Ding L, Xiang C, Zhou G Tags: Talanta Source Type: research

Hepatitis B Antigen Loaded Biodegradable Polymeric Nanoparticles: Formulation Optimization and In-vivo Immunization in BALB/c Mice.
Abstract The incredibly serious problem of Hepatitis B is virus-related chronic liver disease. The conventional preventive treatment of Hepatitis B requires booster dose, which requires repeated administration of the vaccine to the subject. Thus, there is a need to develop a formulation which can eliminate the need of frequent dosing and enhance patient's acquiescence. To prepare single dose nanovaccine of HBsAg by utilizing central composite design for optimization and interaction of independent variables effects on measured response. Nanovaccines were characterized for particle size, morphology, integrity, inter...
Source: Current Drug Delivery - June 3, 2018 Category: Drugs & Pharmacology Authors: Dewangan HK, Singh S, Maurya L, Srivastava A Tags: Curr Drug Deliv Source Type: research

Efficient Encapsulation of Fe3O4 Nanoparticles into Genetically Engineered Hepatitis B Core Virus‐Like Particles Through a Specific Interaction for Potential Bioapplications
Self‐assembly of viral coating proteins encapsulating functional nanoparticles provides a new class of biomaterials with robust chemical and physical properties for potential applications in functional imaging, and therapeutic or diagnostic agent delivery. Herein, a straightforward method is demonstrated for efficient encapsidation of magnetic nanoparticles into the engineered virus‐like particle (VLP) through the affinity of histidine tags for the nickel‐ nitrilotriacetic acid (NTA) chelate. Monodispersed, uniformly sized, magnetic core‐containing VLPs are obtained at high efficiency (>85%) and used as the cell...
Source: Small - August 22, 2014 Category: Nanotechnology Authors: Lihua Shen, Jun Zhou, Yixiao Wang, Ning Kang, Xuebin Ke, Shengli Bi, Lei Ren Tags: Full Paper Source Type: research

A label-free method for the detection of specific DNA sequences using gold nanoparticles bifunctionalized with a chemiluminescent reagent and a catalyst as signal reporters.
Abstract Sensitive, specific, simple, fast, and low-cost DNA detection methods are extremely important in clinical diagnostics, gene therapy, and a variety of biomedical studies. In this work, we developed a general method for the detection of specific DNA sequences from Mycobacterium tuberculosis (TB), hepatitis B virus (HBV), and myelocytomatosis viral oncogene (v-myc) using gold nanoparticles bifunctionalized with both a chemiluminescent (CL) reagent and a catalytic metal complex as signal reporters and a DNA strand complementary to the target as the capture probe. In this CL method, a biotinylated single-stra...
Source: Analytical and Bioanalytical Chemistry - January 11, 2016 Category: Chemistry Authors: Gao L, He X, Ju L, Liu X, Li F, Cui H Tags: Anal Bioanal Chem Source Type: research

Surface-Modified Cobalt Ferrite Nanoparticles for Rapid Capture, Detection, and Removal of Pathogens: a Potential Material for Water Purification.
Abstract Enteric infections resulting from the consumption of contaminated drinking water, inadequate supply of water for personal hygiene, and poor sanitation take a heavy toll worldwide, and developing countries are the major sufferers. Consumption of microbiologically contaminated water leads to diseases such as amoebiasis, cholera, shigellosis, typhoid, and viral infections leading to gastroenteritis and hepatitis B. The present investigation deals with the development of effective method to capture and eliminate microbial contamination of water and improve the quality of water and thus decreasing the contamin...
Source: Applied Biochemistry and Biotechnology - December 1, 2016 Category: Biochemistry Authors: Bohara RA, Throat ND, Mulla NA, Pawar SH Tags: Appl Biochem Biotechnol Source Type: research

Optimization-by-design of hepatotropic lipid nanoparticles targeting the sodium-taurocholate cotransporting polypeptide
Active targeting and specific drug delivery to parenchymal liver cells is a promising strategy to treat various liver disorders. Here, we modified synthetic lipid-based nanoparticles with targeting peptides derived from the hepatitis B virus large envelope protein (HBVpreS) to specifically target the sodium-taurocholate cotransporting polypeptide (NTCP;SLC10A1) on the sinusoidal membrane of hepatocytes. Physicochemical properties of targeted nanoparticles were optimized and NTCP-specific, ligand-dependent binding and internalization was confirmedin vitro. The pharmacokinetics and targeting capacity of selected lead formula...
Source: eLife - July 23, 2019 Category: Biomedical Science Tags: Biochemistry and Chemical Biology Microbiology and Infectious Disease Source Type: research