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Infectious Disease: Hepatitis C

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Total 11868 results found since Jan 2013.

Investigation of novel HCV therapies: Boscia angustifalia & amp;Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment
In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations. The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV. The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %. The release of Boscia angustifalia extract from the nanoparticles was ...
Source: International Journal of Biological Macromolecules - June 23, 2023 Category: Biochemistry Authors: Huda R M Rashdan Hend Okasha Maha M Salem Bothaina M Abd El-Hady Basma Ekram Source Type: research

Anti-Hepatitis C Virus (HCV) and Cytotoxic Activity of Sofosbuvir Decorated Dextran Stabilized Silver Nanoparticles
AbstractSofosbuvir has demonstrated several advantages over the traditional anti-hepatitis C (HCV) therapies; however, it has been found susceptible to hydrolysis and has a risk of hepatic carcinoma. The development of drug nanoparticles allows reduced side effects and increased stability. Silver nanoparticles have long been explored for their intrinsic antiviral characteristics. Therefore, the silver nanoparticles of sofosbuvir were synthesized and capped with various polymers including polyvinylpyrrolidone, polyethylene glycol, and dextran. The stable charachterized surface plasmon resonance band of AgNPs was achieved vi...
Source: Plasmonics - April 20, 2023 Category: Biomedical Science Source Type: research

Protamine-adsorbed magnetic nanoparticles for efficient isolation and concentration of Hepatits-C-Virus from human plasma samples
Chem. Commun., 2013, Accepted ManuscriptDOI: 10.1039/C3CC46793G, CommunicationAbdallah Ahmed Yassin, Ahmed Mohamed Elwaseef, Magdy Elnashar, Johannes Oldenburg, Gunter Mayer, Bernd Potzsch, Jens MullerProtamine hydrochloride adsorbed to citrated superparamagnetic iron oxide nanoparticles represent an efficient tool for capturing and concentration of Hepatitis-C-Virus from plasma samples, improving sensitivity of downstream analysis by nucleic acid...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - November 6, 2013 Category: Chemistry Authors: Abdallah Ahmed Yassin Source Type: research

Protamine-adsorbed magnetic nanoparticles for efficient isolation and concentration of hepatitis-C virus from human plasma samples
Chem. Commun., 2014, 50,590-592DOI: 10.1039/C3CC46793G, CommunicationAbdallah A. Yassin, Ahmed M. Elwaseef, Magdy M. Elnashar, Johannes Oldenburg, Gunter Mayer, Bernd Potzsch, Jens MullerProtamine-adsorbed magnetic nanoparticles allow for efficient isolation of hepatitis-C virus from plasma samples, improving the sensitivity of downstream nucleic acid testing.The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - December 10, 2013 Category: Chemistry Authors: Abdallah A. Yassin Source Type: research

Impedimetric genosensor for detection of hepatitis C virus (HCV1) DNA using viral probe on methylene blue doped silica nanoparticles.
Abstract An impedimetric genosensor was fabricated for detection of hepatitis C virus (HCV) genotype 1 in serum, based on hybridization of the probe with complementary target cDNA from sample. To achieve it, probe DNA complementary to HCVgene was immobilized on the surface of methylene blue (MB) doped silica nanoparticles MB@SiNPs) modified fluorine doped tin oxide (FTO) electrode. The synthesized MB@SiNPs was characterized using scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM) and X-ray diffraction (XRD) pattern. This modified electrode (ssDNA/MB@SiNPs/FTO) served both ...
Source: International Journal of Biological Macromolecules - January 22, 2017 Category: Biochemistry Authors: Singhal C, Ingle A, Chakraborty D, Pn AK, Pundir CS, Narang J Tags: Int J Biol Macromol Source Type: research

Protamine-adsorbed magnetic nanoparticles for efficient isolation and concentration of hepatitis-C virus from human plasma samples.
Abstract Protamine hydrochloride adsorbed onto citrated superparamagnetic iron oxide nanoparticles represents an efficient tool for capturing and concentration of hepatitis-C virus from plasma samples, improving the sensitivity of downstream analysis by nucleic acid testing. PMID: 24275772 [PubMed - as supplied by publisher]
Source: Chemical Communications - November 25, 2013 Category: Chemistry Authors: Yassin AA, Elwaseef AM, Elnashar MM, Oldenburg J, Mayer G, Pötzsch B, Müller J Tags: Chem Commun (Camb) Source Type: research

Lipid Nanoparticles as Carriers for RNAi against Viral Infections: Current Status and Future Perspectives.
Abstract The efforts made to develop RNAi-based therapies have led to productive research in the field of infections in humans, such as hepatitis C virus (HCV), hepatitis B virus (HBV), human immunodeficiency virus (HIV), human cytomegalovirus (HCMV), herpetic keratitis, human papillomavirus, or influenza virus. Naked RNAi molecules are rapidly digested by nucleases in the serum, and due to their negative surface charge, entry into the cell cytoplasm is also hampered, which makes necessary the use of delivery systems to exploit the full potential of RNAi therapeutics. Lipid nanoparticles (LNP) represent one of the...
Source: Biomed Res - September 5, 2014 Category: Research Authors: Torrecilla J, Rodríguez-Gascón A, Solinís MA, Del Pozo-Rodríguez A Tags: Biomed Res Int Source Type: research

Antiviral activity of cuprous oxide nanoparticles against Hepatitis C Virus in vitro.
In conclusion, we report that CO-NPs can act as an anti-HCV agent by targeting the binding of infectious HCV particles to hepatic cells and the virus entry into the cells. These findings suggest that CO-NPs may have novel roles in the treatment of patients with chronic hepatitis C. PMID: 26116793 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - June 25, 2015 Category: Virology Authors: Hang X, Peng H, Song H, Qi Z, Miao X, Xu W Tags: J Virol Methods Source Type: research

Single-Vehicular Delivery of Antagomir and Small Molecules to Inhibit miR-122 Function in Hepatocellular Carcinoma Cells by using "Smart" Mesoporous Silica Nanoparticles.
Abstract MicroRNAs (miRNAs) regulate a variety of biological processes. The liver-specific, highly abundant miR-122 is implicated in many human diseases including cancer. Its inhibition has been found to result in a dramatic loss in the ability of Hepatitis C virus (HCV) to infect host cells. Both antisense technology and small molecules have been used to independently inhibit endogenous miR-122 function, but not in combination. Intracellular stability, efficient delivery, hydrophobicity, and controlled release are some of the current challenges associated with these novel therapeutic methods. Reported herein is t...
Source: Angewandte Chemie - July 15, 2015 Category: Chemistry Authors: Yu C, Qian L, Uttamchandani M, Li L, Yao SQ Tags: Angew Chem Int Ed Engl Source Type: research

Multiplex electrochemiluminescence DNA sensor for determination of hepatitis B virus and hepatitis C virus based on multicolor quantum dots and Au nanoparticles.
Abstract In this work, a novel multiplex electrochemiluminescence (ECL) DNA sensor has been developed for determination of hepatitis B virus (HBV) and hepatitis C virus (HCV) based on multicolor CdTe quantum dots (CdTe QDs) and Au nanoparticles (Au NPs). The electrochemically synthesized graphene nanosheets (GNs) were selected as conducting bridge to anchor CdTe QDs551-capture DNAHBV and CdTe QDs607-capture DNAHCV on the glassy carbon electrode (GCE). Then, different concentrations of target DNAHBV and target DNAHCV were introduced to hybrid with complementary CdTe QDs-capture DNA. Au NPs-probe DNAHBV and Au NPs-p...
Source: Analytica Chimica Acta - March 28, 2016 Category: Chemistry Authors: Liu L, Wang X, Ma Q, Lin Z, Chen S, Li Y, Lu L, Qu H, Su X Tags: Anal Chim Acta Source Type: research

Silencing of hepatitis C virus replication by a non-viral vector based on solid lipid nanoparticles containing a shRNA targeted to the internal ribosome entry site (IRES).
Abstract Gene silencing mediated by RNAi has gained increasing interest as an alternative for the treatment of infectious diseases such as refractory hepatitis C virus (HCV) infection. In this work we have designed and evaluated a non-viral vector based on solid lipid nanoparticles (SLN) bearing hyaluronic acid, protamine and a short hairpin RNA (shRNA74) targeted to the Internal Ribosome Entry Site (IRES) of the HCV. The vector was able to inhibit the expression of the HCV IRES in Huh-7 cells, with the inhibition level dependent on the shRNA74 to SLN ratio and on the shRNA74 dose added to the culture cells. The n...
Source: Colloids and Surfaces - July 11, 2016 Category: Biotechnology Authors: Torrecilla J, Del Pozo-Rodríguez A, Solinís MÁ, Apaolaza PS, Berzal-Herranz B, Romero-López C, Berzal-Herranz A, Rodríguez-Gascón A Tags: Colloids Surf B Biointerfaces Source Type: research

Researchers use gold nanoparticles to deliver hepatitis C vaccine
Researchers at Brigham & Women’s Hospital have developed electrically activated gold nanoparticles that can bore holes into cells’ outer membranes and allow DNA, RNA and proteins to pass through carrying their cargo. The team’s work was published in Advanced Functional Materials. The team tested its technique using a DNA vaccine against hepatitis C in a mice model. Over the course of 3 months, the researchers did not observe any signs of toxicity. The newly developed delivery method induced high levels of anti-hepatitis C virus antibodies and immune cells that secreted inflammatory hormones. Get the...
Source: Mass Device - December 20, 2016 Category: Medical Equipment Authors: Sarah Faulkner Tags: Drug-Device Combinations Pharmaceuticals Research & Development Brigham & Women's Hospital Source Type: news

Enhanced nanoparticle uptake into virus infected cells: Could nanoparticles be useful in antiviral therapy?
In this study, a fluorescently-labelled polymeric nanoparticle was prepared and used to track NP uptake into Huh7.5, human hepatoma cells transfected with replicating HCV genomes, compared with non-transfected cells as a model representing hepatocyte uptake. Confocal microscopy and flow cytometry of virus transfected Huh7.5 cells unexpectedly demonstrated two-fold increase in uptake of NP compared to non-transfected cells. Therefore, virus transfection enhanced NP uptake into Huh7.5 cells and NP could be considered as a promising delivery system for targeted treatment of hepatitis viruses.Graphical abstract
Source: International Journal of Pharmaceutics - July 6, 2018 Category: Drugs & Pharmacology Source Type: research