Biotechnology and Applied Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Metabolite profiling of Piper longum L. fruit volatiles by two ‐dimensional gas chromatography and time‐of‐flight mass spectrometry: Insights into the chemical complexity
AbstractPiper longum L. (long pepper) is an economically and industrially important medicinal plant. However, the characterization of its volatiles has only been analyzed by gas chromatography –mass spectrometry (GC–MS). In the present study, precise characterization ofP. longum fruit volatiles has been performed for the first time through advanced two-dimensional gas chromatography –time-of-flight spectrometry (GC×GC–TOFMS). A total of 146 constituents accounting for 93.79% were identified, of which 30 were reported for the first time. All these constituents were classified into alcohols (4.5%), alkanes (8.9%), a...
Source: Biotechnology and Applied Biochemistry - March 6, 2024 Category: Biochemistry Authors: Manaswini Dash, Subhashree Singh, Suprava Sahoo, Malvika Dutt, Basudeba Kar Tags: ORIGINAL ARTICLE Source Type: research

Selenized lactic acid bacteria microencapsulated by spray drying: A promising strategy for beef cattle feed supplementation
This study aimed to assess the technical feasibility of incorporating selenizedLactobacillus spp. microencapsulated via spray drying into cattle feed. Gum Arabic and maltodextrin were used as encapsulating agents. The encapsulation process was carried out with a drying air flow rate of 1.75  m3/min, inlet air temperature of 90 °C, and outlet air temperature of 75°C. The viability of the encapsulated microorganisms and the technological characteristics of the obtained microparticles were evaluated. Microorganisms were incorporated into beef cattle feed to supplement their diet with up to 0.3 mg of Se per kilogram of fee...
Source: Biotechnology and Applied Biochemistry - March 5, 2024 Category: Biochemistry Authors: Guilherme Schwingel Henn, Larissa Zago Cima, Beatriz Fabris Bettanin, Cl áudia Schlabitz, Daniel Neutzling Lehn, Claucia Fernanda Volken de Souza, Lucélia Hoehne Tags: ORIGINAL ARTICLE Source Type: research

Bacoside ‐A repressed the differentiation and lipid accumulation of 3T3‐L1 preadipocytes by modulating the expression of adipogenic genes
In this study, we assessed the efficacy of bacoside-A (a dammarane-type triterpenoid saponin derived from the plantBacopa monniera Linn.) on the adipogenesis of 3T3-L1 preadipocytes. Results of this study illustrated that bacoside-A decreased the differentiation of 3T3-L1 cell, as evidenced by diminution of lipid droplets, which contains triglycerides and other lipids. During the differentiation process, transcription factors, which are mainly participating in adipogenesis such us CCAAT/enhancer-binding protein α (C/EBPα) and C/EBPβ, peroxisome proliferator-activated receptor-γ (PPARγ), and sterol regulatory element-b...
Source: Biotechnology and Applied Biochemistry - February 29, 2024 Category: Biochemistry Authors: Thiyagarajan Ramesh, Mohammad Shahid Tags: ORIGINAL ARTICLE Source Type: research

Skin wound healing in diabetic rat model using low ‐dose photodynamic therapy
AbstractChronic wound is one of the major challenges in medicine and imposes a heavy financial burden on the healthcare of different countries. Diabetic foot ulcers as one of the important examples for chronic wounds can lead to lower limb amputation, disability, and death in diabetics. In this regard, novel technology with low side effects got attention in recent years. Low-dose photodynamic therapy (LDPDT) is one of the noninvasive techniques that can be considered for wound healing in diabetic wounds. In this experiment, we aim to study the effect of LDPDT on diabetic rats ’ wound healing and compare it to healthy rat...
Source: Biotechnology and Applied Biochemistry - February 27, 2024 Category: Biochemistry Authors: Khatereh Khorsandi, Reza Fekrazad Tags: ORIGINAL ARTICLE Source Type: research

Hsa_circ_0001480 affects osteosarcoma progression by regulating the miR ‐363‐3p/IBSP pathway
In this study, we aimed to determine the role of hsa_circ_0001480 (circ_0001480) in OS development. OS cell invasion, viability, and colony numbers were assessed via transwell, cell counting kit-8, and colony formation assays, respectively. Tumor growth in vivo was also assessed using an OS mouse model. Additionally, targeted associations among the integrin-binding sialoprotein (IBSP), microRNA (miR)-363-3p, and circ_0001480 were evaluated via RNA immunoprecipitation and dual luciferase reporter assays, whereas their expression levels in OS cells and tissues were determined via quantitative reverse transcription-polymerase...
Source: Biotechnology and Applied Biochemistry - February 27, 2024 Category: Biochemistry Authors: Guijuan Bi, Li Zhang Tags: ORIGINAL ARTICLE Source Type: research

Analysis of human epidermal growth factor receptor 2 interaction on aptamer ‐probed interdigitated electrode for breast cancer diagnosis
AbstractBreast cancer has been reported to be high in its incidence with women, and early identification of breast cancer helps to improve and provide an effective treatment. Tumor markers are active substances; in particular, human epidermal growth factor receptor 2 (HER2) is over-expressed at the level of 20% –30%. This research work developed a highly sensitive HER2 biosensor on the interdigitated electrode (IDE) by using aptamer as a detection probe. To enhance the analytical performances, aptamer was attached to the gold nanoparticle and immobilized on the IDE through a chemical linker [(3-aminoprop yl)triethoxysila...
Source: Biotechnology and Applied Biochemistry - February 27, 2024 Category: Biochemistry Authors: Yingxin Hao, Subash C. B. Gopinath Tags: ORIGINAL ARTICLE Source Type: research

Neuroprotective effects of annexin A1 tripeptide in rats with sepsis ‐associated encephalopathy
This study aimed to explore the neuroprotective effects and mechanisms of a novel bioactive ANXA1 tripeptide (ANXA1sp) in SAE. Forty Sprague –Dawley rats were randomly divided into four groups (n = 10 each): control, SAE (intraperitoneal injection of lipopolysaccharide), vehicle (SAE + normal saline), and ANXA1sp (SAE + ANXA1sp) groups. Changes in serum inflammatory factors (interleukin-6 [IL-6], tumor necrosis factor-α [TNF-α]), hippocampal reactive oxygen species (ROS), mitochondr ial membrane potential (MMP), and adenosine triphosphate (ATP) levels were measured. The Morris water maze andY maze tests were used...
Source: Biotechnology and Applied Biochemistry - February 27, 2024 Category: Biochemistry Authors: Qiao Cui, Nannan Qin, Yonghan Zhang, Yanmei Miao, Leiyu Xie, Xinglong Ma, Zhiquan Zhang, Peng Xie Tags: ORIGINAL ARTICLE Source Type: research