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Total 273682 results found since Jan 2013.

Endosperm enzyme activity is responsible for texture and eating quality of cooked rice grains in Japanese cultivars.
Abstract Eating quality of cooked rice grains is an important determinant of its market price and consumer acceptance. To comprehensively assess the variation of eating-quality traits in 152 Japanese rice cultivars, we evaluated activities of eight endosperm enzymes related to degradation of starch and cell-wall polysaccharides. Endosperm enzyme activities showed a wide range of variations and were lower in recently developed cultivars than in landraces and old improved cultivars. Activities of most endosperm enzymes correlated significantly with the eating-quality score and surface texture of cooked rice grains. ...
Source: Bioscience, Biotechnology, and Biochemistry - November 21, 2018 Category: Biochemistry Authors: Iijima K, Suzuki K, Hori K, Ebana K, Kimura K, Tsujii Y, Takano K Tags: Biosci Biotechnol Biochem Source Type: research

A behavioral and pharmacological characterization of palatable diet alternation in mice
In this study, mice were either continuously provided with standard chow food (Chow/Chow), or provided with standard chow for 2days and a high-sucrose, palatable food for 1day (Chow/Palatable). Following stability of intake within the cycling paradigm, we then investigated the effects of several pharmacological treatments on excessive eating of palatable food: naltrexone, an opioid receptor antagonist, SR141716A, a cannabinoid-1 receptor antagonist/inverse agonist, and BD-1063, a sigma-1 receptor antagonist. Over successive cycles, Chow/Palatable mice showed an escalation of palatable food intake within the first hour of r...
Source: Pharmacology Biochemistry and Behavior - December 16, 2017 Category: Biochemistry Source Type: research

Taxonomy, phylogenetics and biogeography of Chesneya (Fabaceae), evidenced from data of three sequences, ITS, trnS-trnG, and rbcL
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Ming-Li Zhang, Zhi-Bin Wen, Xiao-Li Hao, Vyacheslav V. Byalt, Alexander P. Sukhorukov, Stewart C. Sanderson Plants of Central Asia have played a significant role in the origin of floras of Eurasia and the Northern Hemisphere. Chesneya, a small leguminous genus occurring in Central Asia, western Asia, and Tibet, is used to establish phylogenetic relationships and discuss the evolutionary and biogeographical history based on sequence data of ITS and trnS-trnG and rbcL. We employed BEAST Bayesian inference for dating, and ...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

The rise and fall of anandamide: processes that control synthesis, degradation, and storage
Mol Cell Biochem. 2021 Mar 13. doi: 10.1007/s11010-021-04121-5. Online ahead of print.ABSTRACTAnandamide is an endocannabinoid derived from arachidonic acid-containing membrane lipids and has numerous biological functions. Its effects are primarily mediated by the cannabinoid receptors CB1 and CB2, and the vanilloid TRPV1 receptor. Anandamide is known to be involved in sleeping and eating patterns as well as pleasure enhancement and pain relief. This manuscript provides a review of anandamide synthesis, degradation, and storage and hence the homeostasis of the anandamide signaling system.PMID:33713246 | DOI:10.1007/s11010-021-04121-5
Source: Molecular and Cellular Biochemistry - March 13, 2021 Category: Biochemistry Authors: Roger Gregory Biringer Source Type: research

Sportomics: Building a New Concept in Metabolic Studies and Exercise Science.
Abstract For more than a decade, we have used alternative approaches to understand metabolic responses to physical stress. To do so, in addition to classical laboratory studies (cell and animal models), we have used elite athletes and established sports to cause metabolic stress. We proposed a Sportomics approach to mimic both the real challenges and the conditions faced during sports training and competition. Our study is holistic and top-down; we treat data in a systematic way and have generated a large amount of data with a large computational effort.We attempt to understand cell metabolism as an integrated sys...
Source: Biochemical and Biophysical Research communications - January 6, 2014 Category: Biochemistry Authors: Bassini A, Cameron LC Tags: Biochem Biophys Res Commun Source Type: research

Binding of imidazole, 1-methylimidazole and 4-nitroimidazole to yeast cytochrome c peroxidase (CcP) and the distal histidine mutant, CcP(H52L)
Publication date: August 2015 Source:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1854, Issue 8 Author(s): James E. Erman , Diana Chinchilla , Jason Studer , Lidia B. Vitello Imidazole, 1-methylimidazole and 4-nitroimidazole bind to yeast cytochrome c peroxidase (yCcP) with apparent equilibrium dissociation constants (KD app ) of 3.3±0.4, 0.85±0.11, and ~0.2M, respectively, at pH7. This is the weakest imidazole binding to a heme protein reported to date and it is about 120 times weaker than imidazole binding to metmyoglobin. Spectroscopic changes associated with imidazole and 1-methylimidazole ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 10, 2015 Category: Biochemistry Source Type: research

Engaging novice researchers in the process and culture of science using a “Pass‐the‐Problem” case strategy
We describe a “Pass‐the‐Problem” case study approach that engages groups of students in useful discussions about the research environment they are entering. The cases presented here include keeping a thorough laboratory notebook, balancing laboratory and personal time demands, anxiety about formal presentations, unexpected federal regulatory inspection, working in a lab with limited funds, being used as a technician rather than a researcher, frustration with failed experiments, effects of promotion and tenure on laboratory atmosphere, the importance of reading the research literature, and questioning a career in sc...
Source: Biochemistry and Molecular Biology Education - July 7, 2015 Category: Biochemistry Authors: Harold B. White, David C. Usher Tags: Student Centered Education Source Type: research

The stimuli-responsive multiphase behavior of core–shell nanogels with opposite charges and their potential application in in situ gelling system
Publication date: 1 December 2015 Source:Colloids and Surfaces B: Biointerfaces, Volume 136 Author(s): Tin Yu, Shinan Geng, Han Li, Jiangshan Wan, Xiaole Peng, Wei Liu, Yanbing Zhao, Xiangliang Yang, Huibi Xu Concentrated p(N-isopropylacrylamide) (PNIPAM) nanogel dispersions exhibited rich temperature-sensitive sol–gel phase transition behavior. In the present work, the influence of electrostatic forces between nanogel particles, including attraction and repulsion, on the sol–gel phase transition behavior of PNIPAM nanogel dispersions has been studied. Both oppositely charged nanogels with core–shell struc...
Source: Colloids and Surfaces B: Biointerfaces - September 10, 2015 Category: Biochemistry Source Type: research

Stressing Escherichia coli to educate students about research: A CURE to investigate multiple levels of gene regulation
Abstract Course‐based undergraduate research experiences (CUREs) have been shown to increase student retention and learning in the biological sciences. Most CURES cover only one aspect of gene regulation, such as transcriptional control. Here we present a new inquiry‐based lab that engages understanding of gene expression from multiple perspectives. Students carry out a forward genetic screen to identify regulators of the stationary phase master regulator RpoS in the model organism Escherichia coli and then use a series of reporter fusions to determine if the regulation is at the level of transcription or the post‐tr...
Source: Biochemistry and Molecular Biology Education - March 4, 2017 Category: Biochemistry Authors: Janet McDonough, Lara K. Goudsouzian, Agllai Papaj, Ashley R. Maceli, Vanja Klepac ‐Ceraj, Celeste N. Peterson Tags: Curriculum Development Source Type: research

Investigation of the pregnancy-induced muscle bundle dispersal of the inner myometrium of adult mouse uterus and its relationship to the metrial gland/MLAp
In conclusion, we provide novel cellular and spatiotemporal insights about IMD that will contribute to understanding its mechanism and function and allow more informed inter-species comparisons about this process.PMID:34768084 | DOI:10.1016/j.bbrc.2021.11.007
Source: Biochemical and Biophysical Research communications - November 12, 2021 Category: Biochemistry Authors: Artemis Elia Pantelis Georgiades Source Type: research

An introductory classroom exercise on protein molecular model visualization and detailed analysis of protein–ligand binding
A learning module for molecular level analysis of protein structure and ligand/drug interaction through the visualization of X‐ray diffraction is presented. Using DeepView as molecular model visualization software, students learn about the general concepts of protein structure. This Biochemistry classroom exercise is designed to be carried out by following the detailed instructions that make software handling straightforward. Students learn about protein structure and gain insight into the molecular level of the interaction of two active compounds with their receptor. © 2013 by The International Union of Biochemistry an...
Source: Biochemistry and Molecular Biology Education - March 12, 2013 Category: Biochemistry Authors: Poeylaut‐Palena Andrés A., María Laborde Tags: Multimedia in Biochemistry and Molecular Biology Education Source Type: research

Pseudomonas stutzeri Strain Possessing a Self-Transmissible TOL-Like Plasmid Degrades Phenol and Promotes Maize Growth in Contaminated Environments.
Abstract Phenol is volatile organic pollutant that plants can little degrade. For complete degradation of volatile pollutants, we introduced Pseudomonas stutzeri strain P7 to phenol-contaminated soils. The strain effectively degraded phenol and even promoted plant growth. A TOL-like plasmid was detected in the strain and found to be responsible for phenol degradation and self-transmissible. In addition, phenol degradation by strain P7 was more rapid in the contaminated soils with than without plants over the full course of the experiment; especially by 5 days, the phenol concentration was reduced by about 30 % i...
Source: Applied Biochemistry and Biotechnology - February 19, 2014 Category: Biochemistry Authors: Jiang Q, Zhou C, Wang Y, Si F, Zhou Y, Chen B, Zhao Y, Chen J, Xiao M Tags: Appl Biochem Biotechnol Source Type: research

Cloning and Expression of Plantaricin W Produced by Lactobacillus plantarum U10 Isolate from "Tempoyak" Indonesian Fermented Food as Immunity Protein in Lactococcus lactis.
In this study, plnW gene was successfully cloned into vector NICE system pNZ8148 and created the transformant named L. lactis NZ3900 pNZ8148-WU10. PlnW protein was 25.3 kDa in size. The concentration of expressed protein was significantly increased by 10 ng/mL nisin induction. Furthermore, PlnW exhibited protease activity with value of 2.22 ± 0.05 U/mL and specific activity about 1.65 ± 0.03 U/mg protein with 50 ng/mL nisin induction. Immunity study showed that the PlnW had immunity activity especially against plantaricin and rendered L. lactis recombinant an immunity broadly to other bacteriocins such as pedioc...
Source: Applied Biochemistry and Biotechnology - August 15, 2015 Category: Biochemistry Authors: Lages AC, Mustopa AZ, Sukmarini L, Suharsono Tags: Appl Biochem Biotechnol Source Type: research

The enzyme L-isoaspartyl (D-aspartyl) methyltransferase is required for VEGF-dependent endothelial cell migration and tubulogenesis.
Abstract The protein L-isoaspartyl (D-aspartyl) methyltransferase (PIMT) methylates proteins carrying altered aspartyl residues in their structure. PIMT is postulated to limit the accumulation of these damaged proteins with abnormal aspartyl residues. However, little is known about the role of PIMT in tumor growth and almost nothing about its involvement in angiogenic processes. We previously reported that PIMT was up-regulated when endothelial cells were detached from extracellular matrix, leading us to postulate that PIMT could play a critical role during angiogenic steps, since the contacts between endothelial ...
Source: Molecular and Cellular Biochemistry - January 6, 2016 Category: Biochemistry Authors: Ouanouki A, Desrosiers RR Tags: Mol Cell Biochem Source Type: research