Viruses, Vol. 15, Pages 1985: Recent Advances in Structural Studies of Single-Stranded RNA Bacteriophages
e Zhang Positive-sense single-stranded RNA (ssRNA) bacteriophages (phages) were first isolated six decades ago. Since then, extensive research has been conducted on these ssRNA phages, particularly those infecting E. coli. With small genomes of typically 3–4 kb that usually encode four essential proteins, ssRNA phages employ a straightforward infectious cycle involving host adsorption, genome entry, genome replication, phage assembly, and host lysis. Recent advancements in metagenomics and transcriptomics have led to the identification of ~65,000 sequences from ssRNA phages, expanding our understanding of...
Source: Viruses - September 23, 2023 Category: Virology Authors: Jirapat Thongchol Zachary Lill Zachary Hoover Junjie Zhang Tags: Review Source Type: research

Viruses, Vol. 15, Pages 1984: PluriBAC: A Versatile Baculovirus-Based Modular System to Express Heterologous Genes in Different Biotechnological Platforms
tías L. Pidre Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows eno...
Source: Viruses - September 23, 2023 Category: Virology Authors: Leslie C. Amor ós Morales Abril Marchesini Santiago M. G ómez Bergna Mat ías García Fallit Silvana E. Tongiani Larisa V ásquez Mar ía Leticia Ferrelli Guillermo A. Videla-Richardson Marianela Candolfi V íctor Romanowski Mat ías L. Pidre Tags: Article Source Type: research

Viruses, Vol. 15, Pages 1982: Molecular Imaging of ACE2 Expression in Infectious Disease and Cancer
J. Thorek Angiotensin-converting enzyme 2 (ACE2) is a cell-surface receptor that plays a critical role in the pathogenesis of SARS-CoV-2 infection. Through the use of ligands engineered for the receptor, ACE2 imaging has emerged as a valuable tool for preclinical and clinical research. These can be used to visualize the expression and distribution of ACE2 in tissues and cells. A variety of techniques including optical, magnetic resonance, and nuclear medicine contrast agents have been developed and employed in the preclinical setting. Positron-emitting radiotracers for highly sensitive and quantitative tomography have ...
Source: Viruses - September 23, 2023 Category: Virology Authors: Zhiyao Li Abbie Hasson Lasya Daggumati Hanwen Zhang Daniel L. J. Thorek Tags: Review Source Type: research

Viruses, Vol. 15, Pages 1983: Exploring the Comprehensive Kozak Sequence Landscape for AAV Production in Sf9 System
hin The widespread successful use of recombinant Adeno-associated virus (rAAV) in gene therapy has driven the demand for scale-up manufacturing methods of vectors with optimized yield and transduction efficiency. The Baculovirus/Sf9 system is a promising platform for high yield production; however, a major drawback to using an invertebrate cell line compared to a mammalian system is a generally altered AAV capsid stoichiometry resulting in lower biological potency. Here, we introduce a term of the structural and biological “fitness” of an AAV capsid as a function of two interdependent parame...
Source: Viruses - September 23, 2023 Category: Virology Authors: Oleksandr Kondratov Sergei Zolotukhin Tags: Article Source Type: research

Viruses, Vol. 15, Pages 1981: A Baculovirus Expression Vector Derived Entirely from Non-Templated, Chemically Synthesized DNA Parts
Slack Baculovirus expression system1s are a widely used tool in recombinant protein and biologics production. To enable the possibility of genome modifications unconstrained through low-throughput and bespoke classical genome manipulation techniques, we set out to construct a baculovirus vector (>130 kb dsDNA) built from modular, chemically synthesized DNA parts. We constructed a synthetic version of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) through two steps of hierarchical Golden Gate assembly. Over 140 restriction endonuclease sites were removed to enable the discrimination of the synt...
Source: Viruses - September 23, 2023 Category: Virology Authors: Christopher Nguyen Amanda Ibe-Enwo Jeffrey Slack Tags: Communication Source Type: research

Months after hospitalization for COVID-19, MRIs reveal multiorgan damage
This study found abnormal MRI results in 20% to 25% of both controls and hospitalized patients, suggesting “a lot of these cardiac abnormalities that were seen in the COVID group must have been there beforehand.” Post–COVID-19 patients with lingering cardiac symptoms might have disorders like heartbeat irregularities that aren’t always visible with MRI, or dysfunction in other organs, he adds. Although it can offer clues to an organ’s health, MRI is an imperfect measure of how a patient is faring after infection. Researchers found no connection between abnormal liver MRIs and symptoms such as gastroin...
Source: ScienceNOW - September 22, 2023 Category: Science Source Type: news

Hospital based injury and violence prevention programs : ATS Position Statement 2023 - American Trauma Society.
Injury and violence have a significant impact on the health and well-being of the U.S. population and are the leading cause of death for Americans ages 1 to 44.1 In 2021, 224,935 people were killed by unintentional injuries; an additional 48,183 persons di...
Source: SafetyLit - September 22, 2023 Category: International Medicine & Public Health Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news

RSV Vaccine Given in Pregnancy to Help Shield Newborns Receives Full U.S. Approval
FRIDAY, Sept. 22, 2023 -- Women may soon have a vaccine they can take during a pregnancy to help protect their newborn from respiratory syncytial virus (RSV). Following approval one month ago by the U.S. Food and Drug Administration, the Centers...
Source: Drugs.com - Daily MedNews - September 22, 2023 Category: General Medicine Source Type: news

Wamc: 3.64 gpa, 22aa
Date of submission: First week of July Overall GPA: 3.64 Science GPA: 3.53 Bio-Chem-Physics GPA: 3.51 DAT score (include AA and all sections): 22AA 19TS 18PAT 30RC 20BIO 19GC 19OC State of Residence: IL Major: Biology Minority? No Reapplicant? No Nontrad? No Shadowing Experience: general dentist- 90 hours, around 40 from specialties (oral surgeon, orthodontist, endodontist Volunteering Experience: ~400 hours: volunteer cheer coach, animal... Read more
Source: Student Doctor Network - September 22, 2023 Category: Universities & Medical Training Authors: walterskm Tags: What Are My Chances? WAMC Dental Source Type: forums

Good Oral Health Linked to Improved Survival in Head & Neck Cancer
FRIDAY, Sept. 22, 2023 -- For patients with head and neck squamous cell carcinoma, good oral health is associated with improved survival, according to a study published online Sept. 19 in the Journal of the National Cancer Institute. Jason Tasoulas,...
Source: Drugs.com - Pharma News - September 22, 2023 Category: Pharmaceuticals Source Type: news

Morning Exercise May Be Best for Weight Management
FRIDAY, Sept. 22, 2023 -- Morning appears to be the best time of day to exercise for weight management, according to a study published online Sept. 4 in Obesity. Tongyu Ma, M.B.B.S., Ph.D., from Hong Kong Polytechnic University, and colleagues used...
Source: Drugs.com - Pharma News - September 22, 2023 Category: Pharmaceuticals Source Type: news

CMS: Medicaid Reinstated for Nearly 500,000 Children and Families
FRIDAY, Sept. 22, 2023 -- About 500,000 children and families have had their Medicaid reinstated after some states erroneously purged them because of a systems issue, the U.S. Centers for Medicare& Medicaid said Thursday. The agency had first...
Source: Drugs.com - Pharma News - September 22, 2023 Category: Pharmaceuticals Source Type: news

Best home contouring setup
What’s the most ergonomic home contouring setup? - screen setup - input device (trackpad, mouse, stylus) - chair - desk/standing desk I contour a fair amount at home and my Apple trackpad is not very good. I’ve never been satisfied with Apple’s mouse. M1 Mac is great though. I’ve considered iPad/stylus, getting a PC, getting a big TV, etc. What’s worked the best for y’all?
Source: Student Doctor Network - September 22, 2023 Category: Universities & Medical Training Authors: yesmaster Tags: Radiation Oncology Source Type: forums

Ulike Celebrates a Decade of Innovation and Commitment to Cutting-Edge Beauty Technology
10 Years later and 4 Million Devices Sold Globally, Ulike Experiences Rapid Growth To The Top Of The IPL Hair Removal Market NEW YORK, Sept. 22, 2023 /PRNewswire-PRWeb/ -- Ulike, the global leader and pioneer of Sapphire IPL (Intense Pulsed Light) at-home hair removal, is celebrating a...
Source: PRWeb: Medical Pharmaceuticals - September 22, 2023 Category: Pharmaceuticals Tags: PDT Source Type: news

Torn between where to apply?
$(document).ready(function() { window.TSRDataLayer = window.TSRDataLayer || []; window.TSRDataLayer.push({ event: { eventType: 'visible', eventData: { group: 'pm', section: 'pm-content', pmId: , pmTextId: , } } }) $("#reply_pm .post-content a").on("click", function() { window.TSRDataLayer.push({ event: { eventType: 'click', eventData: { group: 'pm', section: 'pm-content', item: 'link', url: this.href, pmId: , pmTextId: , } } }); }); }); ...
Source: The Student Room - September 22, 2023 Category: Universities & Medical Training Authors: Mrmedicine23 Tags: Medicine Source Type: forums

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