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Specialty: Biochemistry
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Total 114746 results found since Jan 2013.
Functionalization of bacterial microcompartment shell interior with cysteine containing peptides enhances the iron and cobalt loading capacity
In this study, we present a method for functionalizing recombinant GRM2-type BMC shell lumens with short cysteine-containing sequences and demonstrate that the iron and cobalt loading capacity of such modified shells is markedly increased. These results also imply that a passive flow of cobalt and iron atoms across the BMC shell could be possible.PMID:37728832 | DOI:10.1007/s10534-023-00538-1
Source: Biometals - September 20, 2023 Category: Biochemistry Authors: Gints Kalnins Maris Bertins Arturs Viksna Kaspars Tars Source Type: research
Improving the Enantioselectivity of CHMO Brevi1 for Asymmetric Synthesis of Podophyllotoxin Precursor
In this study, we use a strategy called "focused rational iterative site-specific mutagenesis" (FRISM) at residues ranging of 6 Å from substrate. The mutations by using a restricted set of rationally chosen amino acids allows the formation of a small mutant library. By generating and screening less than 60 variants, we achieved high ee of 96.8%. Coupled with the cofactor regeneration system, 9.3 mM substrate was converted completely in a 100-mL scale reaction. Therefore, our work reveals a promising synthetic method for (R)-β-piperonyl-γ-butyrolactone with the highest enantioselectivity, and provides a new opportunity f...
Source: Chembiochem - September 20, 2023 Category: Biochemistry Authors: Shou-Cheng Huang Yi-Ke Zhang Qiang Geng Qi-Kang Huang Jian-He Xu Yi-Feng Chen Hui-Lei Yu Source Type: research
Phenolic Substitution in Fidaxomicin: A Semisynthetic Approach to Antibiotic Activity Across Species
Chembiochem. 2023 Sep 20:e202300570. doi: 10.1002/cbic.202300570. Online ahead of print.ABSTRACTFidaxomicin (Fdx) is a natural product antibiotic with potent activity against Clostridioides difficile and other Gram-positive bacteria such as Mycobacterium tuberculosis. Only few Fdx derivatives have been synthesized and examined for their biological activity in the 50 years since its discovery. Fdx has a well-studied mechanism of action, namely inhibition of the bacterial RNA polymerase. Yet, the targeted organisms harbor different target protein sequences, which poses a challenge for the rational development of new semisynt...
Source: Chembiochem - September 20, 2023 Category: Biochemistry Authors: Erik Jung Anastassia Kraimps Silvia Dittmann Tizian Griesser Jordan Costafrolaz Yves Mattenberger Simon Jurt Patrick H Viollier Peter Sander Susanne Sievers Karl Gademann Source Type: research
The conversion of UDP-Glc to UDP-Man: In Silico and Biochemical Exploration to Improve the Catalytic Efficiency of CDP-Tyvelose C2-Epimerases
Chembiochem. 2023 Sep 20:e202300549. doi: 10.1002/cbic.202300549. Online ahead of print.ABSTRACTA promiscuous CDP-tyvelose 2-epimerase (TyvE) from Thermodesulfatator atlanticus (TaTyvE) belonging to the Nucleotide Sugar active Short-Chain Dehydrogenase/Reductase superfamily (NS-SDRs) was recently discovered. TaTyvE performs the slow conversion of NDP-glucose (NDP-Glc) to NDP-mannose (NDP-Man). Here, we present the sequence fingerprints that are indicative of the conversion of UDP-Glc to UDP-Man in TyvE-like enzymes based on the heptagonal box motifs. Our data-mining approach led to the identification of 11 additional TyvE-...
Source: Chembiochem - September 20, 2023 Category: Biochemistry Authors: Ulrike Vogel Matthieu Da Costa Carlos Josue Alvarez Quispe Robin Stragier Henk-Jan Joosten Koen Beerens Tom Desmet Source Type: research
Corrigendum to "CRISPR/Cas9-mediated deletion of large chromosomal segments identifies a minichromosome modulating the Colletotrichum graminicola virulence on maize" Int. J. Biol. Macromol., Volume 245 (2023) 125462
Int J Biol Macromol. 2023 Sep 18:126896. doi: 10.1016/j.ijbiomac.2023.126896. Online ahead of print.NO ABSTRACTPMID:37730523 | DOI:10.1016/j.ijbiomac.2023.126896
Source: International Journal of Biological Macromolecules - September 20, 2023 Category: Biochemistry Authors: Wendi Ma Jun Yang Junqiang Ding Canxing Duan Wensheng Zhao You-Liang Peng Vijai Bhadauria Source Type: research
Preparation of magnetic sodium alginate/sodium carboxymethylcellulose interpenetrating network gel spheres and use in superefficient adsorption of direct dyes in water
Int J Biol Macromol. 2023 Sep 18:126985. doi: 10.1016/j.ijbiomac.2023.126985. Online ahead of print.ABSTRACTThe rapid development of the printing and dyeing industry has led to the production of a large amount of high-density printing and dyeing wastewater, and technology for its effective treatment has become a focus of research. To construct a polymeric adsorbent material with abundant functional groups for the efficient adsorption of dye wastewater, a novel magnetic sodium alginate/carboxymethylcellulose interpenetrating network gel sphere (Fe3O4@SA/CMC-Fe) was prepared by co-blending sodium alginate (SA) and sodium car...
Source: International Journal of Biological Macromolecules - September 20, 2023 Category: Biochemistry Authors: Chen Chen Enhui He Weina Jia Shuwei Xia Liangmin Yu Source Type: research
Remote ischemic preconditioning prevents sarcolemmal-associated proteolysis by MMP-2 inhibition
The objective was to determine if remote ischemic preconditioning (rIPC) preserves membrane-associated cytoskeletal proteins (dystrophin and β-dystroglycan) through the inhibition of metalloproteinase type 2 (MMP-2) activity. A second objective was to describe some of the intracellular signals of the rIPC, that modify mitochondrial function at the early reperfusion. Isolated rat hearts were subjected to 30 min of global ischemia and 120 min of reperfusion (I/R). rIPC was performed by 3 cycles of ischemia/reperfusion in the lower limb (rIPC). rIPC significantly decreased the infarct size, induced Akt/GSK-3 β phosphorylati...
Source: Molecular and Cellular Biochemistry - September 20, 2023 Category: Biochemistry Authors: Eliana P Bin Tamara Zaobornyj Mariana Garces Ver ónica D'Annunzio Bruno Buchholz Timoteo Marchini Pablo Evelson Ricardo J Gelpi Mart ín Donato Source Type: research
Shuttle between arginine and lysine: influence on cancer immunonutrition
Amino Acids. 2023 Sep 20. doi: 10.1007/s00726-023-03327-9. Online ahead of print.ABSTRACTAmino acids which are essential nutrients for all cell types' survival are also recognised to serve as opportunistic/alternative fuels in cancers auxotrophic for specific amino acids. Accordingly, restriction of amino acids has been utilised as a therapeutic strategy in these cancers. Contrastingly, amino acid deficiencies in cancer are found to greatly impair immune functions, increasing mortality and morbidity rates. Dietary and supplemental amino acids in such conditions have revealed their importance as 'immunonutrients' by modulat...
Source: Amino Acids - September 20, 2023 Category: Biochemistry Authors: R Sindhu M Supreeth Shashanka K Prasad M Thanmaya Source Type: research
S-layer is a key element in metabolic response and entry into the stationary phase in Bacillus cereus AH187
In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. Our results demonstrate that the synthesis of the S-layer plays a crucial role in the pathogen's ability to thrive under stationary growth phase conditions by modulating the stress response, thereby promoting its lifestyle as an emetic pathogen. We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches.PMID:37730087 | DOI:10.1016/j.jprot.2023.105007
Source: Journal of Proteomics - September 20, 2023 Category: Biochemistry Authors: C écile Boutonnet Christian Ginies B éatrice Alpha-Bazin Jean Armengaud Alice Ch âteau Catherine Duport Source Type: research
Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under-Utilized Part
Chem Biodivers. 2023 Sep 20:e202301049. doi: 10.1002/cbdv.202301049. Online ahead of print.ABSTRACTMimosa pudica L. (MP) is well-known plant in traditional medicinal system, especially in India. Unfortunately, leaves of MP are less explored. To determine the food and nutritional value of the neglected part of Mimosa pudica L. (MP), that is MP leaves, phytochemicals and metal ions of MP were quantified by newly developed HPLC and ICPOES-based methods. The content of phytochemicals observed using HPLC analysis for chlorogenic acid, catechin, and epicatechin was 141.823 (±8.171), 666.621 (±11.432), and 293.175 (±12.743) μ...
Source: Chemistry and Biodiversity - September 20, 2023 Category: Biochemistry Authors: Ms Yashika Gandhi Shyam Baboo Prasad Vijay Kumar Hemant Soni Hemant Rawat Sujeet K Mishra Jyotika Grewal Simranjeet Singh Vaibhav Charde Akhil Gupta Sudhanshu Kumar Jha Gagandeep Singh Smriti Tandon Akshada Mrkute Praveen C Ramamurthy Ch Venkata Narasimha Source Type: research
Polysaccharide-Based Composite Hydrogel with Hierarchical Microstructure for Enhanced Vascularization and Skull Regeneration
Biomacromolecules. 2023 Sep 20. doi: 10.1021/acs.biomac.3c00655. Online ahead of print.ABSTRACTCritical-size skull defects caused by trauma, infection, and tumor resection raise great demands for efficient bone substitutes. Herein, a hybrid cross-linked hierarchical microporous hydrogel scaffold (PHCLS) was successfully assembled by a multistep procedure, which involved (i) the preparation of poly(lactic-co-glycolic)/nanohydroxyapatite (PLGA-HAP) porous microspheres, (ii) embedding the spheres in a solution of dopamine-modified hyaluronic acid and collagen I (Col I) and cross-linking via dopamine polyphenols binding to (i)...
Source: Biomacromolecules - September 20, 2023 Category: Biochemistry Authors: Gonggong Lu Xiang Li Peilei Wang Xing Li Yuxiang Wang Jiayi Zhu Alfredo Ronca Ugo D'Amora Wenke Liu Xuhui Hui Source Type: research
Comparative Studies of Essential Oil Composition and Biological Activities of Callistemon citrinus from western Himalaya
Chem Biodivers. 2023 Sep 20:e202301129. doi: 10.1002/cbdv.202301129. Online ahead of print.ABSTRACTThis work described a comprehensive study to estimate chemical constituents of essential oils (EOs) extracted from different parts of the Callistemon citrinus viz: fruits, leaves and aerial part. The EOs were characterized using physicochemical parameters, and GC-FID/MS. It was observed that among different parts, aerial part has the highest oil yield (0.90%) followed by leaves and fruits. Further, seventeen compounds were characterized, and represented total amount (97.2-99.5%) with domination of monoterpenes (12.5-34.6%) an...
Source: Chemistry and Biodiversity - September 20, 2023 Category: Biochemistry Authors: Sachin Vashisath Smita Kapoor Vandana Kumari Shabana Butt Dinesh Kumar Source Type: research
Visible Light-Prompted Regioselective Synthesis of Novel 5-Aroyl/hetaroyl-2',4-dimethyl-2,4'-bithiazoles as DNA- and BSA-Targeting Agents
Biomacromolecules. 2023 Sep 20. doi: 10.1021/acs.biomac.3c00554. Online ahead of print.ABSTRACTOrganic transformations mediated by visible light have gained popularity in recent years as they are green, renewable, inexpensive, and clean and yield excellent products. The present study describes cyclo-condensation of 2-methylthiazole-4-carbothioamide with differently substituted α-bromo-1,3-diketones achieved by utilizing a white light-emitting diode (LED) (9W) to accomplish the regioselective synthesis of novel 5-aroyl/hetaroyl-2',4-dimethyl-2,4'-bithiazole derivatives as DNA/bovine serum albumin (BSA)-targeting agents. Th...
Source: Biomacromolecules - September 20, 2023 Category: Biochemistry Authors: Ranjana Aggarwal Naman Jain Gyan Prakash Dubey Snigdha Singh Ramesh Chandra Source Type: research
Assessment of the Alga < em > Cladophora glomerata < /em > as a Source for Cellulose Nanocrystals
Biomacromolecules. 2023 Sep 20. doi: 10.1021/acs.biomac.3c00380. Online ahead of print.ABSTRACTNanocellulose is isolated from cellulosic fibers and exhibits many properties that macroscale cellulose lacks. Cellulose nanocrystals (CNCs) are a subcategory of nanocellulose made of stiff, rodlike, and highly crystalline nanoparticles. Algae of the order Cladophorales are the source of the longest cellulosic nanocrystals, but manufacturing these CNCs is not well-studied. So far, most publications have focused on the applications of this material, with the basic manufacturing parameters and material properties receiving little a...
Source: Biomacromolecules - September 20, 2023 Category: Biochemistry Authors: Karl Mihhels Neptun Yousefi Jaanika Blomster Iina Solala Laleh Solhi Eero Kontturi Source Type: research
Applications and Challenges of Bacteriostatic Aptamers in the Treatment of Common Pathogenic Bacteria Infections
Biomacromolecules. 2023 Sep 20. doi: 10.1021/acs.biomac.3c00634. Online ahead of print.ABSTRACTThe continuous evolution and spread of common pathogenic bacteria is a major challenge in diagnosis and treatment with current biotechnology and modern molecular medicine. To confront this challenge, scientists urgently need to find alternatives for traditional antimicrobial agents. Various bacteriostatic aptamers obtained through SELEX screening are one of the most promising strategies. These bacteriostatic aptamers can reduce bacterial infection by blocking bacterial toxin infiltration, inhibiting biofilm formation, preventing ...
Source: Biomacromolecules - September 20, 2023 Category: Biochemistry Authors: Diandian Li Yuan Su Jie Li Rong Liu Bing Fang Jingjing He Wentao Xu Longjiao Zhu Source Type: research
