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Comparison between radiometry and spectrophotometry for the determination of angiotensin-converting enzyme activity in cerebrospinal fluid
Ann Biol Clin (Paris). 2023 Jun 17;81(3). doi: 10.1684/abc.2023.1809. Online ahead of print.ABSTRACTDetermination of angiotensin-converting enzyme (ACE) activity in cerebrospinal fluid (CSF) can help for establishing the diagnosis of neurosarcoidosis. We investigated the performance characteristics of two assays for ACE determination in 57 CSF, radiometry with [glycine-1-14C] benzoyl-L-histidyl-L-leucine and spectrophotometry with furylacryloyl-phenylalanyl-L-glycyl-L-glycine (FAPGG) as substrates. We compared both kinetic assays to an ELISA specific for human ACE. Within run and between run imprecisions were 14-17% for ra...
Source: Annales de Biologie Clinique - June 17, 2023 Category: Biochemistry Authors: Ludmia Taibi B énédicte Bénéteau-Burnat Michel Vaubourdolle Bruno Baudin Source Type: research
