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A Novel Nested Multiplex Polymerase Chain Reaction Assay for Malaria Diagnosis Using the Hydroxymethyl Dihydropterin Pyrophosphokinase-Dihydropteroate Synthase (hppk-dhps) Gene
Am J Trop Med Hyg. 2023 Sep 11:tpmd230130. doi: 10.4269/ajtmh.23-0130. Online ahead of print.ABSTRACTThere are many techniques for malaria diagnosis. Currently, the nested polymerase chain reaction (PCR) method based on a small subunit ribosomal RNA gene (18S rRNA) has been used as a confirmatory method. However, this method is time-consuming, laborious, and costly. Therefore, the objective of this study was to develop nested multiplex PCR for Plasmodium species identification using the dihydropterin pyrophosphokinase-dihydropteroate synthase (hppk-dhps) gene. Genus- and species-specific primers for the hppk-dhps gene were...
Source: Am J Trop Med Hyg - September 11, 2023 Category: Infectious Diseases Authors: Natpasit Chaianantakul Tippawan Sungkapong Kanokwan Nikhomkham Kansuda Sanseewong Sawanya Kornsang Source Type: research

Biting rhythms and infection rates of anthropophilic sand fly species (Diptera: Phlebotominae) in sites with different land use in southern Mexico
Acta Trop. 2023 Sep 9:107014. doi: 10.1016/j.actatropica.2023.107014. Online ahead of print.ABSTRACTCould tropical forest conversion shape sand fly (Diptera: Phlebotominae) biting rhythms and Leishmania infection rates? Using a Shannon trap, we estimated the bite rate and infection prevalence among anthropophilic sand flies at sites with different land use in southern Mexico. We estimated the expected monthly infection rate of the Leishmania parasite along the gradient and generated information on the biting rhythm of sand flies in a poorly characterized cutaneous leishmaniasis endemic region. We used generalized mixed lin...
Source: Acta Tropica - September 11, 2023 Category: Infectious Diseases Authors: A C Montes de Oca-Aguilar M I Pav ón-Mendez K B L ópez-Ávila E I Sosa-Bibiano E A Rebollar-T éllez J A Palacio-Vargas E A Fern ández-Figueroa E N Lor ía-Cervera Source Type: research

A Novel Nested Multiplex Polymerase Chain Reaction Assay for Malaria Diagnosis Using the Hydroxymethyl Dihydropterin Pyrophosphokinase-Dihydropteroate Synthase (hppk-dhps) Gene
Am J Trop Med Hyg. 2023 Sep 11:tpmd230130. doi: 10.4269/ajtmh.23-0130. Online ahead of print.ABSTRACTThere are many techniques for malaria diagnosis. Currently, the nested polymerase chain reaction (PCR) method based on a small subunit ribosomal RNA gene (18S rRNA) has been used as a confirmatory method. However, this method is time-consuming, laborious, and costly. Therefore, the objective of this study was to develop nested multiplex PCR for Plasmodium species identification using the dihydropterin pyrophosphokinase-dihydropteroate synthase (hppk-dhps) gene. Genus- and species-specific primers for the hppk-dhps gene were...
Source: The American Journal of Tropical Medicine and Hygiene - September 11, 2023 Category: Tropical Medicine Authors: Natpasit Chaianantakul Tippawan Sungkapong Kanokwan Nikhomkham Kansuda Sanseewong Sawanya Kornsang Source Type: research

Biting rhythms and infection rates of anthropophilic sand fly species (Diptera: Phlebotominae) in sites with different land use in southern Mexico
Acta Trop. 2023 Sep 9:107014. doi: 10.1016/j.actatropica.2023.107014. Online ahead of print.ABSTRACTCould tropical forest conversion shape sand fly (Diptera: Phlebotominae) biting rhythms and Leishmania infection rates? Using a Shannon trap, we estimated the bite rate and infection prevalence among anthropophilic sand flies at sites with different land use in southern Mexico. We estimated the expected monthly infection rate of the Leishmania parasite along the gradient and generated information on the biting rhythm of sand flies in a poorly characterized cutaneous leishmaniasis endemic region. We used generalized mixed lin...
Source: Acta Tropica - September 11, 2023 Category: Infectious Diseases Authors: A C Montes de Oca-Aguilar M I Pav ón-Mendez K B L ópez-Ávila E I Sosa-Bibiano E A Rebollar-T éllez J A Palacio-Vargas E A Fern ández-Figueroa E N Lor ía-Cervera Source Type: research

A Novel Nested Multiplex Polymerase Chain Reaction Assay for Malaria Diagnosis Using the Hydroxymethyl Dihydropterin Pyrophosphokinase-Dihydropteroate Synthase (hppk-dhps) Gene
Am J Trop Med Hyg. 2023 Sep 11:tpmd230130. doi: 10.4269/ajtmh.23-0130. Online ahead of print.ABSTRACTThere are many techniques for malaria diagnosis. Currently, the nested polymerase chain reaction (PCR) method based on a small subunit ribosomal RNA gene (18S rRNA) has been used as a confirmatory method. However, this method is time-consuming, laborious, and costly. Therefore, the objective of this study was to develop nested multiplex PCR for Plasmodium species identification using the dihydropterin pyrophosphokinase-dihydropteroate synthase (hppk-dhps) gene. Genus- and species-specific primers for the hppk-dhps gene were...
Source: Am J Trop Med Hyg - September 11, 2023 Category: Infectious Diseases Authors: Natpasit Chaianantakul Tippawan Sungkapong Kanokwan Nikhomkham Kansuda Sanseewong Sawanya Kornsang Source Type: research

Inhibition of carbonic anhydrase using aspirin is a novel method to block schistosomiasis infection of the parasitic trematode, Schistosoma mansoni, in the intermediate snail host, Biomphalaria glabrata
In this study, we investigated the role of CA inhibition in B. glabrata snails exposed to S. mansoni miracidia. We analyzed the expression of the B. glabrata CA encoding transcript in juvenile susceptible and resistant snails, with and without exposure to S. mansoni. Our results showed that the expression of the CA mRNA encoding transcript was upregulated during early and prolonged infection in susceptible snails (BBO2), but not in the resistant BS-90 stock. Notably, sodium salicylate, a form of aspirin, inhibited the expression of CA, post-exposure, to the parasite. Increasing research between parasites and cancer has sho...
Source: Experimental Parasitology - September 11, 2023 Category: Parasitology Authors: Simone Parn Gabriela Lewis Matty Knight Source Type: research

A Novel Nested Multiplex Polymerase Chain Reaction Assay for Malaria Diagnosis Using the Hydroxymethyl Dihydropterin Pyrophosphokinase-Dihydropteroate Synthase (hppk-dhps) Gene
Am J Trop Med Hyg. 2023 Sep 11:tpmd230130. doi: 10.4269/ajtmh.23-0130. Online ahead of print.ABSTRACTThere are many techniques for malaria diagnosis. Currently, the nested polymerase chain reaction (PCR) method based on a small subunit ribosomal RNA gene (18S rRNA) has been used as a confirmatory method. However, this method is time-consuming, laborious, and costly. Therefore, the objective of this study was to develop nested multiplex PCR for Plasmodium species identification using the dihydropterin pyrophosphokinase-dihydropteroate synthase (hppk-dhps) gene. Genus- and species-specific primers for the hppk-dhps gene were...
Source: The American Journal of Tropical Medicine and Hygiene - September 11, 2023 Category: Tropical Medicine Authors: Natpasit Chaianantakul Tippawan Sungkapong Kanokwan Nikhomkham Kansuda Sanseewong Sawanya Kornsang Source Type: research

Inhibition of carbonic anhydrase using aspirin is a novel method to block schistosomiasis infection of the parasitic trematode, Schistosoma mansoni, in the intermediate snail host, Biomphalaria glabrata
In this study, we investigated the role of CA inhibition in B. glabrata snails exposed to S. mansoni miracidia. We analyzed the expression of the B. glabrata CA encoding transcript in juvenile susceptible and resistant snails, with and without exposure to S. mansoni. Our results showed that the expression of the CA mRNA encoding transcript was upregulated during early and prolonged infection in susceptible snails (BBO2), but not in the resistant BS-90 stock. Notably, sodium salicylate, a form of aspirin, inhibited the expression of CA, post-exposure, to the parasite. Increasing research between parasites and cancer has sho...
Source: Experimental Parasitology - September 11, 2023 Category: Parasitology Authors: Simone Parn Gabriela Lewis Matty Knight Source Type: research

A Novel Nested Multiplex Polymerase Chain Reaction Assay for Malaria Diagnosis Using the Hydroxymethyl Dihydropterin Pyrophosphokinase-Dihydropteroate Synthase (hppk-dhps) Gene
Am J Trop Med Hyg. 2023 Sep 11:tpmd230130. doi: 10.4269/ajtmh.23-0130. Online ahead of print.ABSTRACTThere are many techniques for malaria diagnosis. Currently, the nested polymerase chain reaction (PCR) method based on a small subunit ribosomal RNA gene (18S rRNA) has been used as a confirmatory method. However, this method is time-consuming, laborious, and costly. Therefore, the objective of this study was to develop nested multiplex PCR for Plasmodium species identification using the dihydropterin pyrophosphokinase-dihydropteroate synthase (hppk-dhps) gene. Genus- and species-specific primers for the hppk-dhps gene were...
Source: The American Journal of Tropical Medicine and Hygiene - September 11, 2023 Category: Tropical Medicine Authors: Natpasit Chaianantakul Tippawan Sungkapong Kanokwan Nikhomkham Kansuda Sanseewong Sawanya Kornsang Source Type: research

The interplay between toxoplasmosis and host miRNAs: Mechanisms and consequences
Pathol Res Pract. 2023 Sep 6;250:154790. doi: 10.1016/j.prp.2023.154790. Online ahead of print.ABSTRACTToxoplasmosis is one of the highly prevalent zoonotic diseases worldwide caused by the parasite Toxoplasma gondii (T. gondii). The infection with T. gondii could pass unidentified in immunocompetent individuals; however, latent cysts remain dormant in their digestive tract, but they could be shed and excreted with feces infesting the environment. However, active toxoplasmosis can create serious consequences, particularly in newborns and infected persons with compromised immunity. These complications include ocular toxopla...
Source: Pathology, Research and Practice - September 8, 2023 Category: Pathology Authors: Ahmed S Doghish Mohamed A Ali Mahmoud A Elrebehy Hend H Mohamed Reda Mansour Aml Ghanem Ahmed Hassan Mohammed S Elballal Ola Elazazy Ahmed E Elesawy Sherif S Abdel Mageed Yara A Nassar Osama A Mohammed Ahmed I Abulsoud Source Type: research

Clinical features of infectious uveitis in a Tunisian population
CONCLUSION: This study highlighted the causes and clinical features of IU in our setting. Tuberculosis remains one of its most frequent etiologies in Tunisia. However, a larger multicenter national study would allow a more precise evaluation.PMID:37682270
Source: Herpes - September 8, 2023 Category: Infectious Diseases Authors: Rym Maamouri Meriam Beldi Yasmine Houman Mehdi Somai Fatma Boussema Monia Cheour Source Type: research

Genetic and phenotypic parameters for sexual precocity and parasite resistance traits in Nellore cattle
J Appl Genet. 2023 Sep 8. doi: 10.1007/s13353-023-00781-9. Online ahead of print.ABSTRACTIndicator traits of sexual precocity are widely used as selection criteria for the genetic improvement of beef cattle; however, the impact of selection for these traits on resistance to endoparasites and ectoparasites is unknown. Therefore, this study aimed to estimate the genetic and phenotypic parameters for indicator traits of sexual precocity and parasite resistance in Nellore cattle. The sexual precocity traits evaluated were probability of first calving (PFC) and scrotal circumference at 12 and 18 months of age (SC12 and SC18). T...
Source: J Appl Genet - September 8, 2023 Category: Genetics & Stem Cells Authors: Mariana Mamedes de Moraes Gabriela Canabrava Gouveia Virg ínia Mara Pereira Ribeiro Andresa Eva Melo de Ara újo Fabio Luiz Buranelo Toral Eduardo Penteado Cardoso Source Type: research