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Deciphering mechanisms of < em > bla < /em > < sub > NDM < /sub > gene transmission between human and animals: a genomics study of bacterial isolates from various sources in China, 2015 to 2017
ConclusionThis study in a Chinese context suggests that cross-species transmission of certain STs of E. coli harbouring bla NDM on mobile elements, may facilitate the spread of carbapenem-resistant Enterobacteriaceae. Stringent monitoring of bla NDM-bearing E. coli in ecosystems is important.PMID:37707983 | DOI:10.2807/1560-7917.ES.2023.28.37.2200925
Source: Euro Surveill - September 14, 2023 Category: Infectious Diseases Authors: Kaichao Chen Miaomiao Xie Ning Dong Edward Wai Chi Chan Rong Zhang Sheng Chen Source Type: research

One-tube RPA-CRISPR Cas12a/Cas13a rapid detection of methicillin-resistant Staphylococcus aureus
Anal Chim Acta. 2023 Oct 16;1278:341757. doi: 10.1016/j.aca.2023.341757. Epub 2023 Aug 26.ABSTRACTAt present, methicillin-resistant Staphylococcus aureus (MRSA) has caused a serious impact on a global scale. The infection and carrier rate of MRSA in the community is increasing year by year, but there is still no convenient detection system for on-site rapid detection. It is very important to select a rapid detection system to accurately and quickly detect patients infected with MRSA. We have developed a high-efficient single-tube detection platform based on RPA and CRISPR reaction system to detect the genes of mecA and clf...
Source: Analytica Chimica Acta - September 14, 2023 Category: Chemistry Authors: Yujie Liu Hui Liu Guanliu Yu Wenbo Sun Muhammad Aizaz Guiwen Yang Lei Chen Source Type: research

Approaches for high-throughput quantification of periplasmic recombinant proteins
N Biotechnol. 2023 Sep 12;77:149-160. doi: 10.1016/j.nbt.2023.09.003. Online ahead of print.ABSTRACTThe Gram-negative periplasm is a convenient location for the accumulation of many recombinant proteins including biopharmaceutical products. It is the site of disulphide bond formation, required by some proteins (such as antibody fragments) for correct folding and function. It also permits simpler protein release and downstream processing than cytoplasmic accumulation. As such, targeting of recombinant proteins to the E. coli periplasm is a key strategy in biologic manufacture. However, expression and translocation of each r...
Source: New Biotechnology - September 14, 2023 Category: Biotechnology Authors: Alexander Osgerby Tim W Overton Source Type: research

Photo-Electro-Biochemical H2 Production Using the Carbon Material-Based Cathode Combined with Genetically Engineered Escherichia coli Whole-Cell Biocatalysis
This study constructed a H2-forming biocathode based on a carbon material combined with whole-cell biocatalysis of genetically-engineered-hydrogenase-overproducing Escherichia coli for the photoelectrochemical water splitting for clean H2 production. Low-cost and abundant carbon materials are generally not suitable for H2-forming cathode due to their high overpotential for proton reduction; however, the combination of the reduction of an organic electron mediator on the carbon electrode and the H2 formation with the reduced mediator by the redox enzyme hydrogenase provides a H2-forming cathodic reaction comparable to that ...
Source: ChemSusChem - September 14, 2023 Category: Chemistry Authors: Yuki Honda Risa Yuki Reina Hamakawa Hiroshi Fujii Source Type: research

Deciphering mechanisms of < em > bla < /em > < sub > NDM < /sub > gene transmission between human and animals: a genomics study of bacterial isolates from various sources in China, 2015 to 2017
ConclusionThis study in a Chinese context suggests that cross-species transmission of certain STs of E. coli harbouring bla NDM on mobile elements, may facilitate the spread of carbapenem-resistant Enterobacteriaceae. Stringent monitoring of bla NDM-bearing E. coli in ecosystems is important.PMID:37707983 | DOI:10.2807/1560-7917.ES.2023.28.37.2200925
Source: Euro Surveill - September 14, 2023 Category: Infectious Diseases Authors: Kaichao Chen Miaomiao Xie Ning Dong Edward Wai Chi Chan Rong Zhang Sheng Chen Source Type: research

One-tube RPA-CRISPR Cas12a/Cas13a rapid detection of methicillin-resistant Staphylococcus aureus
Anal Chim Acta. 2023 Oct 16;1278:341757. doi: 10.1016/j.aca.2023.341757. Epub 2023 Aug 26.ABSTRACTAt present, methicillin-resistant Staphylococcus aureus (MRSA) has caused a serious impact on a global scale. The infection and carrier rate of MRSA in the community is increasing year by year, but there is still no convenient detection system for on-site rapid detection. It is very important to select a rapid detection system to accurately and quickly detect patients infected with MRSA. We have developed a high-efficient single-tube detection platform based on RPA and CRISPR reaction system to detect the genes of mecA and clf...
Source: Analytica Chimica Acta - September 14, 2023 Category: Chemistry Authors: Yujie Liu Hui Liu Guanliu Yu Wenbo Sun Muhammad Aizaz Guiwen Yang Lei Chen Source Type: research

Deciphering mechanisms of < em > bla < /em > < sub > NDM < /sub > gene transmission between human and animals: a genomics study of bacterial isolates from various sources in China, 2015 to 2017
ConclusionThis study in a Chinese context suggests that cross-species transmission of certain STs of E. coli harbouring bla NDM on mobile elements, may facilitate the spread of carbapenem-resistant Enterobacteriaceae. Stringent monitoring of bla NDM-bearing E. coli in ecosystems is important.PMID:37707983 | DOI:10.2807/1560-7917.ES.2023.28.37.2200925
Source: Euro Surveill - September 14, 2023 Category: Infectious Diseases Authors: Kaichao Chen Miaomiao Xie Ning Dong Edward Wai Chi Chan Rong Zhang Sheng Chen Source Type: research

One-tube RPA-CRISPR Cas12a/Cas13a rapid detection of methicillin-resistant Staphylococcus aureus
Anal Chim Acta. 2023 Oct 16;1278:341757. doi: 10.1016/j.aca.2023.341757. Epub 2023 Aug 26.ABSTRACTAt present, methicillin-resistant Staphylococcus aureus (MRSA) has caused a serious impact on a global scale. The infection and carrier rate of MRSA in the community is increasing year by year, but there is still no convenient detection system for on-site rapid detection. It is very important to select a rapid detection system to accurately and quickly detect patients infected with MRSA. We have developed a high-efficient single-tube detection platform based on RPA and CRISPR reaction system to detect the genes of mecA and clf...
Source: Analytica Chimica Acta - September 14, 2023 Category: Chemistry Authors: Yujie Liu Hui Liu Guanliu Yu Wenbo Sun Muhammad Aizaz Guiwen Yang Lei Chen Source Type: research

A metallothionein gene from hard clam Meretrix meretrix: Sequence features, expression patterns, and metal tolerance activities
Dev Comp Immunol. 2023 Sep 12:105057. doi: 10.1016/j.dci.2023.105057. Online ahead of print.ABSTRACTMetallothioneins (MTs) are low-molecular weight cytoplasmic heavy metal binding proteins. MTs can regulate the concentration of essential or non-essential metals in organisms, and have many important biological functions, including detoxification, trace element metabolism, and anti-oxidation. In the present study, we cloned and characterized a metallothionein gene (designated as MmMT) from the hard clam Meretrix meretrix. The complete cDNA sequence of MmMT contained an open reading frame (ORF) of 629 bp, which encoded a prot...
Source: Developmental and Comparative Immunology - September 14, 2023 Category: Allergy & Immunology Authors: Jing Yang Ying Guo Jingjie Hu Zhenmin Bao Mengqiang Wang Source Type: research

Time evolution of electrical impedance spectra of Staphylococcus aureus and Escherichia coli bacteria
Bioelectrochemistry. 2023 Sep 9;155:108557. doi: 10.1016/j.bioelechem.2023.108557. Online ahead of print.ABSTRACTThis research work reports the time evolution of the electrical properties of gram-positive and gram-negative bacteria in aqueous suspensions with methyl violet and Lugol; measurements of galvanostatic electrical impedance spectra were made in a frequency range of 10Hz to 100kHz. The magnitude of the impedance as a function of frequency for methicillin-resistant strains, Staphylococcus aureus (gram-positive), and Escherichia coli O157: H7 (gram-negative) in the presence of methyl violet and Lugol, showed that bo...
Source: Bioelectrochemistry - September 14, 2023 Category: Biochemistry Authors: David Alejandro Miranda Mercado Erika Viviana Godoy Alarc ón Ely Dannier V-Ni ño Source Type: research

Photo-Electro-Biochemical H2 Production Using the Carbon Material-Based Cathode Combined with Genetically Engineered Escherichia coli Whole-Cell Biocatalysis
This study constructed a H2-forming biocathode based on a carbon material combined with whole-cell biocatalysis of genetically-engineered-hydrogenase-overproducing Escherichia coli for the photoelectrochemical water splitting for clean H2 production. Low-cost and abundant carbon materials are generally not suitable for H2-forming cathode due to their high overpotential for proton reduction; however, the combination of the reduction of an organic electron mediator on the carbon electrode and the H2 formation with the reduced mediator by the redox enzyme hydrogenase provides a H2-forming cathodic reaction comparable to that ...
Source: ChemSusChem - September 14, 2023 Category: Chemistry Authors: Yuki Honda Risa Yuki Reina Hamakawa Hiroshi Fujii Source Type: research

Phenotypic and genotypic characterizations of bacteria isolated from the respiratory microbiota of healthy turkeys with potential for probiotic composition
AbstractDesirable characteristics ofStaphylococcus sp.,Streptococcus sp.,Bacillus sp.,Klebsiella sp.,Escherichia coli, andPseudomonas pseudoalcaligenes isolated from the trachea of healthy turkeys were evaluated as probiotic candidates in the search for new alternatives to solve antimicrobial resistance issues in poultry. In current study phenotypic and genotypic capacity to produce bacteriocin-like substances, efficacy to inhibit the growth of avian pathogens, susceptibility to antimicrobials of bacteria isolated from the respiratory microbiota of healthy turkeys, and the presence of virulence-associated genes (VAGs) pred...
Source: Veterinary Research Communications - September 14, 2023 Category: Veterinary Research Source Type: research

Assessment of chemical compositions and antibacterial activity of the essential oil of < em > Mentha piperita < /em > in response to salicylic acid
Nat Prod Res. 2023 Sep 13:1-12. doi: 10.1080/14786419.2023.2256020. Online ahead of print.ABSTRACTMentha piperita, as a species of the mint family, is used in various medical, cosmetic, industrial and culinary products. The effect of different concentrations of salicylic acid (SA) (0. 0.5, 1 mM) on phytochemical activity and antimicrobial potential of Mentha piperita essential oil against six human pathogenic bacteria (Streptococcus agalactiae, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Listeria monocytogenes) were investigated. According to the results to prevent the growth ...
Source: Natural Product Research - September 13, 2023 Category: Biochemistry Authors: Soheila Afkar Source Type: research