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Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation
Identifying regulators of human pluripotent stem cell differentiation using Fluorescence Ubiquitin Cell Cycle Indicator technology. Schematic representation of the Fluorescence Ubiquitin Cell Cycle Indicator technology labeling individual late G1 phase nuclei in red and S/G2/M phase nuclei in green, whereas early G1 phase nuclei are double negative. Study design using H9 ‐Fluorescence Ubiquitin Cell Cycle Indicator human pluripotent stem cells treated with or without 2% dimethylsulfoxide for 24 hours followed by cell cycle sorting and high throughput RNA‐sequencing to identify regulators of proliferation and differen...
Source: Stem Cells - June 9, 2019 Category: Stem Cells Authors: Anna Shcherbina, Jingling Li, Cyndhavi Narayanan, William Greenleaf, Anshul Kundaje, Sundari Chetty Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Cancer, stem cell misplacement and cancer stem cells.
Abstract The cell of origin of cancer as well as cancer stem cells is still a mystery. In a recent issue of JCMM, Wang et al. challenged the conventional somatic genetic mutation model of multi-stage carcinogenesis of breast cancer and proposed that 'Invasive cancers are not necessary from preformed in situ tumours-an alternative way of carcinogenesis from misplaced stem cells'. If this stem cell misplacement theory could withstand future experimental evaluation, it may provide a paradigm shift in the prevention and management of cancer in the clinic. PMID: 23855541 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - July 16, 2013 Category: Molecular Biology Authors: Liao Y Tags: J Cell Mol Med Source Type: research

Dynamic heterogeneity of DNA methylation and hydroxymethylation in embryonic stem cell populations captured by single-cell 3D high-content analysis.
Abstract Cell-surface markers and transcription factors are being used in the assessment of stem cell fate and therapeutic safety, but display significant variability in stem cell cultures. We assessed nuclear patterns of 5-hydroxymethylcytosine (5hmC, associated with pluripotency), a second important epigenetic mark, and its combination with 5-methylcytosine (5mC, associated with differentiation), also in comparison to more established markers of pluripotency (Oct-4) and endodermal differentiation (FoxA2, Sox17) in mouse embryonic stem cells (mESC) over a 10-day differentiation course in vitro: by means of confoc...
Source: Experimental Cell Research - February 17, 2015 Category: Cytology Authors: Tajbakhsh J, Stefanovski D, Tang G, Wawrowsky K, Liu N, Fair JH Tags: Exp Cell Res Source Type: research

Cell Competition Modifies Adult Stem Cell and Tissue Population Dynamics in a JAK-STAT-Dependent Manner.
Abstract Throughout their lifetime, cells may suffer insults that reduce their fitness and disrupt their function, and it is unclear how these potentially harmful cells are managed in adult tissues. We address this question using the adult Drosophila posterior midgut as a model of homeostatic tissue and ribosomal Minute mutations to reduce fitness in groups of cells. We take a quantitative approach combining lineage tracing and biophysical modeling and address how cell competition affects stem cell and tissue population dynamics. We show that healthy cells induce clonal extinction in weak tissues, targeting both...
Source: Developmental Cell - July 22, 2015 Category: Cytology Authors: Kolahgar G, Suijkerbuijk SJ, Kucinski I, Poirier EZ, Mansour S, Simons BD, Piddini E Tags: Dev Cell Source Type: research

Perivascular Mesenchymal Stem Cells From the Adult Human Brain Harbor No Instrinsic Neuroectodermal but High Mesodermal Differentiation Potential
This study showed the lack of an innate neuronal but high mesodermal differentiation potential. Because of their relationship to mesenchymal stem cells, these adult brain perivascular mesodermal cells are of great interest for possible autologous therapeutic use.
Source: Stem Cells Translational Medicine - September 17, 2015 Category: Stem Cells Authors: Lojewski, X., Srimasorn, S., Rauh, J., Francke, S., Wobus, M., Taylor, V., Arauzo-Bravo, M. J., Hallmeyer-Elgner, S., Kirsch, M., Schwarz, S., Schwarz, J., Storch, A., Hermann, A. Tags: Tissue Engineering and Regenerative Medicine, Perivascular Stem Cells Source Type: research

A Cell-ebration of Induced Pluripotency.
Abstract PAPERCLIP. PMID: 26849296 [PubMed - as supplied by publisher]
Source: Cell Stem Cell - February 4, 2016 Category: Stem Cells Authors: editorial teams of Cell and Cell Stem Cell Tags: Cell Stem Cell Source Type: research

Motor Neuron Transdifferentiation of Neural Stem Cell from Adipose-Derived Stem Cell Characterized by Differential Gene Expression.
Abstract Adipose-derived stem cells (ADSC) are adult stem cells which can be induced into motor neuron-like cells (MNLC) with a preinduction-induction protocol. The purpose of this study is to generate MNLC from neural stem cells (NSC) derived from ADSC. The latter were isolated from the perinephric regions of Sprague-Dawley rats, transdifferentiated into neurospheres (NS) using B27, EGF, and bFGF. After generating NSC from the NS, they induced into MNLC by treating them with Shh and RA, then with GDNF, CNTF, BDNF, and NT-3. The ADSC lineage was evaluated by its mesodermal differentiation and was characterized by ...
Source: Cellular and Molecular Neurobiology - April 22, 2016 Category: Cytology Authors: Darvishi M, Tiraihi T, Mesbah-Namin SA, Delshad A, Taheri T Tags: Cell Mol Neurobiol Source Type: research

Characterization of stem-like cells in a new astroblastoma cell line.
Abstract Cell lines established from tumors are the most commonly used models in cancer research, and their use in recent years has enabled a greater understanding of the biology of cancer and the means to develop effective treatment strategies. Astroblastomas are uncommon neuroepithelial tumors of glial origin, predominantly affecting young people, mainly teenagers and children, predominantly females. To date, only a single study has reported that astroblastomas contain a large number of neural stem-like cells, which had only a partial proliferation capacity and differentiation. Our objective was to establish an ...
Source: Experimental Cell Research - February 19, 2017 Category: Cytology Authors: Coban EA, Kasikci E, Karatas OF, Suakar O, Kuskucu A, Altunbek M, Türe U, Sahin F, Bayrak OF Tags: Exp Cell Res Source Type: research

Human Mesenchymal Stem Cells Promote CD34(+) Hematopoietic Stem Cell Proliferation with Preserved Red Blood Cell Differentiation Capacity.
This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control. Cell expansion rates, number of mononuclear cell post-expansion and number of enucleated cells post-differentiation were evaluated. HSCs showed superior proliferation in the presence of MSC with mean expansion rate of 3.5 x 10(8) ± 1.8 x 10(7) after day 7 compared to the conditioned medium and the control group (8.9 x 10(7) ± 1.1 x 10(8) and 7.0 x 10(7) ± 3.3 x 10(6) respectively, p<0.001). A...
Source: Cell Biology International - April 12, 2017 Category: Cytology Authors: Lau SX, Leong YY, Ng WH, Po Ng AW, Ismail IS, Yusoff NM, Ramasamy R, Tan JJ Tags: Cell Biol Int Source Type: research

Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Diseases 2017. An Official American Thoracic Society Workshop Report.
Abstract The University of Vermont Larner College of Medicine, in collaboration with the National Heart, Lung, and Blood Institute (NHLBI), the Alpha-1 Foundation, the American Thoracic Society, the Cystic Fibrosis Foundation, the European Respiratory Society, the International Society for Cell & Gene Therapy, and the Pulmonary Fibrosis Foundation, convened a workshop titled "Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Diseases" from July 24 through 27, 2017, at the University of Vermont, Burlington, Vermont. The conference objectives were to review and discuss current understanding of t...
Source: Am J Respir Cell Mol... - September 30, 2019 Category: Respiratory Medicine Authors: Ryan AL, Ikonomou L, Atarod S, Bölükbas DA, Collins J, Freishtat R, Hawkins F, Gilpin SE, Uhl FE, Uriarte JJ, Weiss DJ, Wagner DE Tags: Am J Respir Cell Mol Biol Source Type: research

Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases
Methods Mol Biol. 2021 Dec 15. doi: 10.1007/7651_2021_452. Online ahead of print.ABSTRACTHere, we describe a protocol for reprogramming of bone marrow-derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease.PMID:34907509 | DOI:10.1007/7651_2021_452
Source: Mol Biol Cell - December 15, 2021 Category: Molecular Biology Authors: G ülen Güney-Esken Fatima Aerts-Kaya Source Type: research

Hedgehog Signaling is Dispensable in the Proliferation of Stem Cells from Human Exfoliated Deciduous Teeth.
Abstract The hedgehog (Hh) signaling pathway is one of the key regulators in development with a dual role in cell fate specification, proliferation, and survival on different target cells. We have investigated the effect of recombinant sonic hedgehog (r-SHH) on extracted multipotent stem cells from human exfoliated deciduous teeth (SHED), which represent a potential stem cell population for therapeutic applications. Cell proliferation and cycle assays shown that r-SHH did not have a distinctive effect on cell cycle progression, nor did it increase cell number over a wide range of concentrations. Quantitative polym...
Source: Cell Biology International - December 18, 2013 Category: Cytology Authors: Ejeian F, Baharvand H, Nasr-Esfahani MH Tags: Cell Biol Int Source Type: research

Comparative analysis of mesenchymal stem cell and embryonic tendon progenitor cell response to embryonic tendon biochemical and mechanical factors.
CONCLUSIONS: Similar responses as TPCs to specific treatments suggest MSCs have tenogenic potential. Potentially shared mechanisms of cell function between MSCs and TPCs should be investigated in longer term studies. PMID: 25956970 [PubMed - as supplied by publisher]
Source: Cell Research - May 11, 2015 Category: Cytology Authors: Brown JP, Galassi TV, Stoppato M, Schiele NR, Kuo CK Tags: Stem Cell Res Ther Source Type: research