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Development and evaluation of multi-epitope protein p72 (MeP72) for the serodiagnosis of African swine fever
Acta Virol. 2021;65(3):273-278. doi: 10.4149/av_2021_304.ABSTRACTAfrican swine fever (ASF) is an acute and severe infectious disease that seriously endangers the global porcine industry. In order to develop ASF serodiagnostic reagents with high specificity and sensitivity, in the present study, the antigenic epitopes of P72 protein of African swine fever virus (ASFV) were analyzed, and the ASFV multi-epitope fusion gene MeP72 in tandem with the dominant linear epitopes was constructed. The recombinant multi-epitope fusion MeP72 (reMeP72) was prepared in Escherichia coli. A colloidal gold-based immunochromatographic assay (...
Source: Acta Virologica - September 27, 2021 Category: Infectious Diseases Authors: Xingxing Zhang Jing Guo Lixia Wang Zhiyuan Li Yucheng Liu Lulu Tian Chencheng Xiao Yanfang Li Xuepeng Cai Qingling Meng Jun Qiao Source Type: research
Intestinal microbiota disturbance affects the occurrence of African swine fever
In conclusion, African swine fever could alter the abundance of dominant bacteria in pigs, and Treponema may be one of the important inducers for swine pathogenicity. HighlightsThe bacterial population composition in sick pigs and healthy pigs was basically similar, but the relative abundance of dominant bacteria was significantly difference.ASF could alter the abundance of dominant bacteria in pigs, and Treponema may be one of the important inducers for swine pathogenicity.These results will provide further evidence for the ASF infection in local pig farms and provide reference for their microecological control, which has...
Source: Animal Biotechnology - December 7, 2021 Category: Biotechnology Authors: Cheng-Xing Long Jie-Qi Wu Zhou-Jin Tan Source Type: research
Reverse Genetics for Influenza A and B Viruses Driven by Swine Polymerase I Promoter
Methods Mol Biol. 2022;2465:257-281. doi: 10.1007/978-1-0716-2168-4_14.ABSTRACTInfluenza viruses are considered prominent pathogens of humans and animals that are extensively investigated because of public health importance. Plasmid-based reverse genetics is a fundamental tool that facilitates the generation of genetically modified viruses from a cDNA copy. The ability to rescue viruses enables researchers to understand different biological characteristics including IV replication, pathogenesis, and transmission. Furthermore, understanding the biology and ability to manipulate different aspects of the virus can aid in prov...
Source: Mol Biol Cell - February 4, 2022 Category: Molecular Biology Authors: Brittany Seibert Stivalis Cardenas-Garcia Daniela Rajao Daniel R Perez Source Type: research
Altered cerebrovascular regulation in low birthweight swine
Comp Biochem Physiol A Mol Integr Physiol. 2022 Feb 10:111163. doi: 10.1016/j.cbpa.2022.111163. Online ahead of print.ABSTRACTFull-term low birthweight (LBW) offspring exhibit peripheral vascular dysfunction in the postnatal period; however, whether such impairments extend to the cerebrovasculature remains to be elucidated. We used a swine model to test the hypothesis that LBW offspring would exhibit cerebrovascular dysfunction at later stages of life. Offspring from 14 sows were identified as normal birthweight (NBW) or LBW and were assessed at 28 (similar to end of infancy) and 56 (similar to childhood) days of age. LBW ...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - February 13, 2022 Category: Physiology Authors: Jude S Morton Breanna Patton Cameron J Morse Zeyad El Karsh Lucas A Rodrigues Darrell D Mousseau David P Ferguson Daniel A Columbus Lynn P Weber T Dylan Olver Source Type: research
Development of a p72 trimer-based colloidal gold strip for detection of antibodies against African swine fever virus
Appl Microbiol Biotechnol. 2022 Mar 15:1-12. doi: 10.1007/s00253-022-11851-z. Online ahead of print.ABSTRACTAfrican swine fever virus (ASFV) causes a highly contagious and often lethal swine viral disease, and leads to tremendous economic losses to the swine industry. Unfortunately, there are no vaccines and effective antiviral agents available to prevent and control ASFV outbreaks. Therefore, it is necessary to develop simple and rapid strategies to monitor ASFV-infected pigs to restrain its spread. In the current study, ASFV capsid protein p72 was expressed along with its chaperone pB602L to form trimers in human embryon...
Source: Applied Microbiology and Biotechnology - March 15, 2022 Category: Microbiology Authors: Rui Geng Yaning Sun Rui Li Jifei Yang Hongfang Ma Zixuan Qiao Qingxia Lu Songlin Qiao Gaiping Zhang Source Type: research
Serological Surveillance of the H1N1 and H3N2 Swine Influenza A Virus in Chinese Swine between 2016 and 2021
CONCLUSIONS: The results of this study showed that the anti-EA H1N1 subtype and pdm/09 H1N1 subtype antibodies were readily detected in swine serum samples. The EA H1N1 subtype has become dominant in the pig population. The occurrences of coinfections from two or more subtypes afforded opportunities for their reassortment to produce new viruses. Our findings emphasized the need for continuous surveillance of influenza viruses.PMID:35528158 | PMC:PMC9071888 | DOI:10.1155/2022/5833769
Source: Biomed Res - May 9, 2022 Category: Research Authors: Yuzhong Zhao Lebin Han Ting Chen Haotian Sang Guofei Ding Yingchao Li Bin Wang Liting Qin Sidang Liu Yanmeng Hou Yihong Xiao Source Type: research
Synergistic Inactivation of African Swine Fever Virus by a Highly Complexed Iodine Combined with Compound Organic Acids
This study evaluated the synergistic inactivation effects of HPCI combined with compound organic acids (COAs) against ASFV. The results showed that the inactivation rates of HPCI, COAs, and HPCI+COAs on the reporter ASFV expressing the green fluorescent protein increased in dose- and time-dependent manners. The best inactivation effects were obtained when the compatibility ratio of HPCI and COAs was 5:1, and the ideal temperature was 25°C. Furthermore, there were no significant differences when comparing the efficacy of HPCI combined with COAs (HPCI+COAs) in inactivating wild-type ASFV and the reporter ASFV (P > 0.05)....
Source: Applied and Environmental Microbiology - May 19, 2022 Category: Microbiology Authors: Mengnan Qi Li Pan Ying Gao Miao Li Yanjin Wang Lian-Feng Li Chen Ji Yuan Sun Hua-Ji Qiu Source Type: research
