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Viruses, Vol. 14, Pages 179: Rapid Detection of Genotype II African Swine Fever Virus Using CRISPR Cas13a-Based Lateral Flow Strip
In this study, we developed a rapid and sensitive ASFV detection method based on the collateral cleavage activity of LwCas13a, which combines recombinase-aided amplification (RAA) and a lateral flow strip (named CRISPR/Cas13a-LFD). The method was an isothermal detection at 37 °C, and the detection can be used for visual readout. The detection limit of the CRISPR/Cas13a-LFD was 101 copies/µL of p72 gene per reaction, and the detection process can be completed within an hour. The assay showed no cross-reactivity to eight other swine viruses, including classical swine fever virus (CSFV), and has a 10...
Source: Viruses - January 18, 2022 Category: Virology Authors: Ning Wei Bohan Zheng Junjun Niu Tao Chen Jing Ye Youhui Si Shengbo Cao Tags: Article Source Type: research
Concurrent determination of cyanide and thiocyanate in human and swine antemortem and postmortem blood by high-performance liquid chromatography-tandem mass spectrometry
Anal Bioanal Chem. 2023 Sep 15. doi: 10.1007/s00216-023-04939-6. Online ahead of print.ABSTRACTCyanide (in the form of cyanide anion (CN-) or hydrogen cyanide (HCN), inclusively represented as CN) can be a rapidly acting and deadly poison, but it is also a common chemical component of a variety of natural and anthropogenic substances. The main mechanism of acute CN toxicity is based on blocking terminal electron transfer by inhibiting cytochrome c oxidase, resulting in cellular hypoxia, cytotoxic anoxia, and potential death. Due to the well-established link between blood CN concentrations and the manifestation of symptoms,...
Source: Analytical and Bioanalytical Chemistry - September 15, 2023 Category: Chemistry Authors: Abdullah H Alluhayb Carter Severance Tara Hendry-Hofer Vikhyat S Bebarta Brian A Logue Source Type: research
Swine Flu Vaccination Does Not Increase Risk Of Miscarriage
The idea that the swine flu vaccine given to Norwegian women in 2009 increased their risk of pregnancy loss has been dismissed as false according to a recent study carried out in The New England Journal of Medicine. Swine flu is a highly contagious respiratory disease of pigs, spread by direct and indirect contact. Its symptoms are very similar to those of a typical seasonal flu: body aches, cough, chills, temperature, headache and sore throat. Detection of the disease is often overlooked unless experts are specifically searching for it...
Source: Health News from Medical News Today - January 18, 2013 Category: Consumer Health News Tags: Swine Flu Source Type: news
2009 Swine Flu Pandemic Infected 1 In Every 5 People
Data gathered from 19 different countries reveals that the global swine flu pandemic which began in 2009 infected close to 20% of the population and almost 50% of all schoolchildren. The virus which originated in Mexico is thought to have killed close to 200,000. Swine flu is a respiratory disease commonly found in pigs - around 1 to 4 percent of pigs that develop the disease die from it. It is caused by a highly contagious strain of Influenza A virus and it's spread by direct and indirect contact with pigs...
Source: Health News from Medical News Today - January 25, 2013 Category: Consumer Health News Tags: Swine Flu Source Type: news
Development of a gold nanoparticle-based oligonucleotide microarray for simultaneous detection of seven swine viruses.
Abstract
A gold nanoparticle (GNP) based oligonucleotide microarray assay (GNMA) that combined GNP-based multiplex asymmetric PCR with silver enhancement detection, was developed for simultaneous detection of seven important swine viruses in intensive swine production. Multiplex PCR was first performed to enable the target fragments of seven viruses containing a universal sequence, which were labeled simultaneously with GNPs by multiplex asymmetric PCR in the presence of excess GNP-conjugated primer. Target labeled products were captured by virus-specific oligonucleotide probes immobilized on microarrays, followed...
Source: Journal of Virological Methods - May 23, 2013 Category: Virology Authors: Wang X, Dang E, Gao J, Guo S, Li Z Tags: J Virol Methods Source Type: research
Academics linked to drug industry 'exaggerated' swine flu risk
New research published in the BMJ has suggested that academics with links to the pharmaceutical industry were more likely to give increased risk assessments of the swine flu pandemic of 2009/10 when talking to the media, compared with academics who were not linked to the pharmaceutical industry. Swine flu is a highly contagious respiratory disease found in pigs. The H1N1 influenza subtype is the type that has been known to infect humans, with outbreaks occurring worldwide throughout 2009 and 2010...
Source: Health News from Medical News Today - November 12, 2013 Category: Consumer Health News Tags: Swine Flu Source Type: news
Request for Proposal to Collect Positive Samples for Foot and Mouth Disease, African Swine Fever, and Classical Swine Fever
Funding Opportunity Number: USDA-GRANTS-07012014-001 Opportunity Category: DiscretionaryFunding Instrument Type: Cooperative AgreementCategory of Funding Activity: AgricultureCFDA Number: 10.025Eligible Applicants Others (see text field entitled "Additional Information on Eligibility" for clarification)Agency Name: USDA-APHISClosing Date: Sep 01, 2014Award Ceiling: Expected Number of Awards: 5Creation Date: Jul 01, 2014Funding Opportunity Description: In an effort to enhance the sample types and test platforms available for African Swine Fever (ASF), Classic Swine Fever (CSF) and Foot and Mouth Disease (FMD...
Source: Grants.gov - July 1, 2014 Category: Research Tags: Agriculture Source Type: funding
Reducing Neointima Formation in a Swine Model with IVUS and Sirolimus Microbubbles.
Abstract
Potent therapeutic compounds with dose dependent side effects require more efficient and selective drug delivery to reduce systemic drug doses. Here, we demonstrate a new platform that combines intravascular ultrasound (IVUS) and drug-loaded microbubbles to enhance and localize drug delivery, while enabling versatility of drug type and dosing. Localization and degree of delivery with IVUS and microbubbles was assessed using fluorophore-loaded microbubbles and different IVUS parameters in ex vivo swine arteries. Using a swine model of neointimal hyperplasia, reduction of neointima formation following ballo...
Source: Annals of Biomedical Engineering - April 17, 2015 Category: Biomedical Engineering Authors: Kilroy JP, Dhanaliwala AH, Klibanov AL, Bowles DK, Wamhoff BR, Hossack JA Tags: Ann Biomed Eng Source Type: research
Swine flu jab and narcolepsy may be linked by autoimmune response
Vaccine may have caused narcolepsy by triggering the production of antibodies which destroy a sleep-regulating part of the brain, a new study suggestsScientists appear close to pinpointing why a swine flu vaccine given to six million people in Britain triggered the devastating sleep disorder, narcolepsy, in rare cases.The Pandemrix vaccine, made by GlaxoSmithKline (GSK), and which was recommended by the UK government to high-risk groups during the 2009-10 swine flu pandemic, was withdrawn after medical records showed a spike in the number of cases of narcolepsy presenting in doctors’ surgeries. Continue reading...
Source: Guardian Unlimited Science - July 1, 2015 Category: Science Authors: Hannah Devlin, science correspondent Tags: Swine flu Science Health Vaccines and immunisation Health policy UK news Source Type: news
Intravenous Lipid Emulsion Therapy for Severe Diphenhydramine Toxicity: A Randomized, Controlled Pilot Study in a Swine Model
Conclusion In our study of diphenhydramine-induced hypotensive swine, we found no difference in hypotension, QRS widening, or diphenhydramine levels in aqueous layers between intravenous lipid emulsion and sodium bicarbonate.
Source: Annals of Emergency Medicine - July 10, 2015 Category: Emergency Medicine Source Type: research
Swine flu reaches 'epidemic' proportions in St Petersburg
Dozens have died in a seasonal outbreak of the killer flu virus sweeping across former Soviet countries
Source: The Telegraph : Swine Flu A H1N1 - January 25, 2016 Category: Infectious Diseases Tags: Russia St Petersburg Ukraine Moscow Soviet swine flu vaccine eastern Ukraine swine flu Source Type: news
The effects of sternal intraosseous and intravenous administration of amiodarone in a hypovolemic swine cardiac arrest model.
CONCLUSION: The SIO provides rapid and reliable access to administer life-saving medications during cardiac arrest.
PMID: 28140442 [PubMed - in process]
Source: American Journal of Disaster Medicine - February 2, 2017 Category: Global & Universal Tags: Am J Disaster Med Source Type: research
Ministers lose fight to stop payouts over swine flu jab narcolepsy cases
Dozens of children who developed sleep disorder after getting vaccine could get compensation after high court rulingDozens of children who developed narcolepsy as a result of a swine flu vaccine could be compensated after the high court rejecteda government appeal to withhold payments.Six million people in Britain, and more across Europe, were given the Pandemrix vaccine made by GlaxoSmithKline during the 2009-10 swine flu pandemic, but the jab was withdrawn after doctors noticed asharp rise in narcolepsy among those who received it.Continue reading...
Source: Guardian Unlimited Science - February 9, 2017 Category: Science Authors: Ian Sample Science editor Tags: Science Vaccines and immunisation Swine flu Health Society World news UK news Source Type: news
