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Expression and immunogenicity of recombinant porcine epidemic diarrhea virus Nsp9
In this study, PEDV Nsp9 protein fused with MBP protein and His-tag were expressed and purified in Escherichia coli. Furthermore, immunization of MBP-Nsp9 enhances both humoral and cellular immunity responses as compared with that of His-Nsp9 protein. Finally, the swine immunization showed that Nsp9 protein could stimulate the swine immunity system to carry out humoral immunity, and the generated antibody could inhibit the proliferation of PEDV in Vero cells. Altogether, our data provide direct evidence for the immunogenicity of PEDV Nsp9, which sheds light on the future developments of anti-PEDV drugs and vaccines for PED...
Source: Virology - August 12, 2023 Category: Virology Authors: Shiguo Liu Qijia Yu Sha Li Mingzhi Li Li Yang Quansheng Wang Zewen Tu Feifei Tao Pingping Yang Lingbao Kong Xiu Xin Source Type: research
Ultra-sensitive photoelectrochemical biosensor for determination of African swine fever virus based on surface plasmon resonance
In this study, a bismuth induced enhanced photoelectrochemical (PEC) biosensor based on in-situ loop mediated isothermal amplification (LAMP) was constructed using deposited bismuth nanoparticles loaded bismuth oxycarbonate (Bi/(BiO)2CO3) as photoactive material, using primers designed according to LAMP as recognition elements, and using in-situ LAMP to achieve nucleic acid amplification of target genes. As the Bi induced surface plasmon resonance (SPR) effect, enhanced light captures and effective electron hole separation, it could effectively enhance the photoelectric activity, so the prepared Bi/(BiO)2CO3 nanohybrid had...
Source: Analytica Chimica Acta - August 12, 2023 Category: Chemistry Authors: Ruishuang Yuan Jie Wei Rui Geng Bin Li Wei Xiong Xueen Fang Peng L ü Kun Wang Source Type: research
Expression and immunogenicity of recombinant porcine epidemic diarrhea virus Nsp9
In this study, PEDV Nsp9 protein fused with MBP protein and His-tag were expressed and purified in Escherichia coli. Furthermore, immunization of MBP-Nsp9 enhances both humoral and cellular immunity responses as compared with that of His-Nsp9 protein. Finally, the swine immunization showed that Nsp9 protein could stimulate the swine immunity system to carry out humoral immunity, and the generated antibody could inhibit the proliferation of PEDV in Vero cells. Altogether, our data provide direct evidence for the immunogenicity of PEDV Nsp9, which sheds light on the future developments of anti-PEDV drugs and vaccines for PED...
Source: Virology - August 12, 2023 Category: Virology Authors: Shiguo Liu Qijia Yu Sha Li Mingzhi Li Li Yang Quansheng Wang Zewen Tu Feifei Tao Pingping Yang Lingbao Kong Xiu Xin Source Type: research
Ultra-sensitive photoelectrochemical biosensor for determination of African swine fever virus based on surface plasmon resonance
In this study, a bismuth induced enhanced photoelectrochemical (PEC) biosensor based on in-situ loop mediated isothermal amplification (LAMP) was constructed using deposited bismuth nanoparticles loaded bismuth oxycarbonate (Bi/(BiO)2CO3) as photoactive material, using primers designed according to LAMP as recognition elements, and using in-situ LAMP to achieve nucleic acid amplification of target genes. As the Bi induced surface plasmon resonance (SPR) effect, enhanced light captures and effective electron hole separation, it could effectively enhance the photoelectric activity, so the prepared Bi/(BiO)2CO3 nanohybrid had...
Source: Analytica Chimica Acta - August 12, 2023 Category: Chemistry Authors: Ruishuang Yuan Jie Wei Rui Geng Bin Li Wei Xiong Xueen Fang Peng L ü Kun Wang Source Type: research
Expression and immunogenicity of recombinant porcine epidemic diarrhea virus Nsp9
In this study, PEDV Nsp9 protein fused with MBP protein and His-tag were expressed and purified in Escherichia coli. Furthermore, immunization of MBP-Nsp9 enhances both humoral and cellular immunity responses as compared with that of His-Nsp9 protein. Finally, the swine immunization showed that Nsp9 protein could stimulate the swine immunity system to carry out humoral immunity, and the generated antibody could inhibit the proliferation of PEDV in Vero cells. Altogether, our data provide direct evidence for the immunogenicity of PEDV Nsp9, which sheds light on the future developments of anti-PEDV drugs and vaccines for PED...
Source: Virology - August 12, 2023 Category: Virology Authors: Shiguo Liu Qijia Yu Sha Li Mingzhi Li Li Yang Quansheng Wang Zewen Tu Feifei Tao Pingping Yang Lingbao Kong Xiu Xin Source Type: research
Ultra-sensitive photoelectrochemical biosensor for determination of African swine fever virus based on surface plasmon resonance
In this study, a bismuth induced enhanced photoelectrochemical (PEC) biosensor based on in-situ loop mediated isothermal amplification (LAMP) was constructed using deposited bismuth nanoparticles loaded bismuth oxycarbonate (Bi/(BiO)2CO3) as photoactive material, using primers designed according to LAMP as recognition elements, and using in-situ LAMP to achieve nucleic acid amplification of target genes. As the Bi induced surface plasmon resonance (SPR) effect, enhanced light captures and effective electron hole separation, it could effectively enhance the photoelectric activity, so the prepared Bi/(BiO)2CO3 nanohybrid had...
Source: Analytica Chimica Acta - August 12, 2023 Category: Chemistry Authors: Ruishuang Yuan Jie Wei Rui Geng Bin Li Wei Xiong Xueen Fang Peng L ü Kun Wang Source Type: research
Expression and immunogenicity of recombinant porcine epidemic diarrhea virus Nsp9
In this study, PEDV Nsp9 protein fused with MBP protein and His-tag were expressed and purified in Escherichia coli. Furthermore, immunization of MBP-Nsp9 enhances both humoral and cellular immunity responses as compared with that of His-Nsp9 protein. Finally, the swine immunization showed that Nsp9 protein could stimulate the swine immunity system to carry out humoral immunity, and the generated antibody could inhibit the proliferation of PEDV in Vero cells. Altogether, our data provide direct evidence for the immunogenicity of PEDV Nsp9, which sheds light on the future developments of anti-PEDV drugs and vaccines for PED...
Source: Virology - August 12, 2023 Category: Virology Authors: Shiguo Liu Qijia Yu Sha Li Mingzhi Li Li Yang Quansheng Wang Zewen Tu Feifei Tao Pingping Yang Lingbao Kong Xiu Xin Source Type: research
Ultra-sensitive photoelectrochemical biosensor for determination of African swine fever virus based on surface plasmon resonance
In this study, a bismuth induced enhanced photoelectrochemical (PEC) biosensor based on in-situ loop mediated isothermal amplification (LAMP) was constructed using deposited bismuth nanoparticles loaded bismuth oxycarbonate (Bi/(BiO)2CO3) as photoactive material, using primers designed according to LAMP as recognition elements, and using in-situ LAMP to achieve nucleic acid amplification of target genes. As the Bi induced surface plasmon resonance (SPR) effect, enhanced light captures and effective electron hole separation, it could effectively enhance the photoelectric activity, so the prepared Bi/(BiO)2CO3 nanohybrid had...
Source: Analytica Chimica Acta - August 12, 2023 Category: Chemistry Authors: Ruishuang Yuan Jie Wei Rui Geng Bin Li Wei Xiong Xueen Fang Peng L ü Kun Wang Source Type: research
Improvement of weanling pigs immune status and metabolic condition using ultraweak light
In conclusion, the use of Photonia for weanling pigs is recommended due to improved G:F, immune status and activation of amino acids metabolic pathways includingd-glutamine,d-glutamate, alanine, aspartate, glutamate and phenylalanine.
Source: Journal of Animal Physiology and Animal Nutrition - August 12, 2023 Category: Zoology Authors: Chang Beon Lee,
Abdolreza Hosseindoust,
Sang Hun Ha,
Jun Young Mun,
Joseph N. Moturi,
Habeeb Tajudeen,
Mi Jung Park,
Jeong Su Yang,
Sang Chul Lee,
Jin Soo Kim Tags: ORIGINAL ARTICLE Source Type: research
Viruses, Vol. 15, Pages 1722: The 2022 Outbreaks of African Swine Fever Virus Demonstrate the First Report of Genotype II in Ghana
In this study, ASF isolates were collected from samples from swine suspected of dying from ASF on farms in Ghana in early 2022. While previous studies determined that the circulating strains in Ghana were p72 Genotype I, we demonstrate here that the strains circulating in 2022 were derivatives of the p72 Genotype II pandemic strain. Therefore, this study demonstrates for the first time the emergence of Genotype II ASFV in Ghana.
Source: Viruses - August 11, 2023 Category: Virology Authors: Edward Spinard Ayushi Rai Jehadi Osei-Bonsu Vivian O ’Donnell Patrick T. Ababio Daniel Tawiah-Yingar Daniel Arthur Daniel Baah Elizabeth Ramirez-Medina Nallely Espinoza Alyssa Valladares Bonto Faburay Aruna Ambagala Theophlius Odoom Manuel V. Borca Doug Tags: Article Source Type: research
Molecular breeding of livestock for disease resistance
Virology. 2023 Aug 4;587:109862. doi: 10.1016/j.virol.2023.109862. Online ahead of print.ABSTRACTAnimal infectious diseases pose a significant threat to the global agriculture and biomedicine industries, leading to significant economic losses and public health risks. The emergence and spread of viral infections such as African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and avian influenza virus (AIV) have highlighted the need for innovative approaches to develop resilient and disease-resistant animal populations. Gene editing technologies, ...
Source: Virology - August 10, 2023 Category: Virology Authors: Fei Gao Pan Li Ye Yin Xuguang Du Gengsheng Cao Sen Wu Yaofeng Zhao Source Type: research
