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Prokaryotic expression of porcine deltacoronavirus S gene truncated segment and establishment of indirect ELISA detection method
This study aimed to establish an indirect ELISA method for detecting PDCoV antibodies using the truncated gene of PDCoV spike protein (S). The purified S protein was used as the coating antigen for the polyclonal antibody. The conditions were optimized to establish an indirect ELISA detection method for PDCoV based on the S protein, which showed good specificity and no cross-reaction with SVV-VP1, ASFV-P72, GETV-E2, PRV-gE, etc. The method has high repeatability, with coefficients of variation within and between batches less than 10%. Compared with the commercial kit, the positive coincidence rate is 86.40%, the negative c...
Source: Journal of Virological Methods - July 23, 2023 Category: Virology Authors: Lan Chen Yao Huang Tong Xu Si-Yuan Lai Yan-Ru Ai Yuancheng Zhou Ling Zhu Zhi-Wen Xu Source Type: research

Viruses, Vol. 15, Pages 1602: Porcine Circovirus 2 Increases the Frequency of Transforming Growth Factor- & beta; via the C35, S36 and V39 Amino Acids of the ORF4
Viruses, Vol. 15, Pages 1602: Porcine Circovirus 2 Increases the Frequency of Transforming Growth Factor-β via the C35, S36 and V39 Amino Acids of the ORF4 Viruses doi: 10.3390/v15071602 Authors: Cheng Han Weicheng Xu Jianfang Wang Xiaolin Hou Shuanghai Zhou Qinye Song Xuewei Liu Huanrong Li Porcine circovirus 2 (PCV2) is one of the most important endemic swine pathogens, inducing immunosuppression in pigs and predisposing them to secondary bacterial or viral infections. Our previous studies show that PCV2 infection stimulated pig intestinal epithelial cells (IPEC-J2) to produce the secretory...
Source: Viruses - July 22, 2023 Category: Virology Authors: Cheng Han Weicheng Xu Jianfang Wang Xiaolin Hou Shuanghai Zhou Qinye Song Xuewei Liu Huanrong Li Tags: Article Source Type: research

Development of an RT-PCR-based RspCas13d  system to detect porcine deltacoronavirus
Appl Microbiol Biotechnol. 2023 Jul 21. doi: 10.1007/s00253-023-12690-2. Online ahead of print.ABSTRACTPorcine deltacoronavirus (PDCoV) is an enteropathogen that causes diarrhea in piglets and may undergo cross-species transmission. The prevention and control of PDCoV are complicated, and a sensitive, specific, and accessible method of diagnosis would be advantageous. Whereas qPCR is a standard approach for detecting PDCoV, it is not effectively sensitive. In the present study, we report such a strategy using an RT-PCR-based RspCas13d detection system and its efficacy in clinical sample diagnosis. The detection limit of th...
Source: Applied Microbiology and Biotechnology - July 21, 2023 Category: Microbiology Authors: Yu Zhao Tiejun Zhang Changyu Zhou Peng Ma Kui Gu Hao Li Wenjing Li Xin Yang Hongning Wang Source Type: research

Role of a typical swine liquid manure treatment plant in reducing elements of antibiotic resistance
Environ Sci Pollut Res Int. 2023 Jul 21. doi: 10.1007/s11356-023-28823-z. Online ahead of print.ABSTRACTBiological treatment of swine liquid manure may be a favorable environment for the enrichment of bacteria carrying antibiotic resistance genes (ARGs), raising the alert about this public health problem. The present work sought to investigate the performance of a swine wastewater treatment plant (SWWTP), composed of a covered lagoon biodigester (CLB) followed by three facultative ponds, in the removal of usual pollutants, antibiotics, ARGs (blaTEM, ermB, qnrB, sul1, and tetA), and intI1. The SWWTP promoted a 70% of organi...
Source: Environmental Science and Pollution Research International - July 21, 2023 Category: Environmental Health Authors: Andressa Rezende Pereira Lucimeire de Ávila Barbosa Fonseca Aline Gomes de Oliveira Paranhos Camila Cristina Rodrigues Ferreira da Cunha S érgio Francisco de Aquino Silvana de Queiroz Silva Source Type: research

Viruses, Vol. 15, Pages 1591: Nicotinamide Efficiently Suppresses Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus Replication
In this study, we investigated the effects of nicotinamide (NAM) on PEDV and PDCoV replication and found that NAM treatment significantly inhibited PEDV and PDCoV reproduction. Moreover, NAM plays an important role in replication processes. NAM primarily inhibited PEDV and PDCoV RNA and protein synthesis rather than other processes. Furthermore, we discovered that NAM treatment likely inhibits the replication of PEDV and PDCoV by downregulating the expression of transcription factors through activation of the ERK1/2/MAPK pathway. Overall, this study is the first to suggest that NAM might be not only an important antiviral ...
Source: Viruses - July 21, 2023 Category: Virology Authors: Mingxia Li Liping Zhang Li Pan Peng Zhou Ruiming Yu Zhongwang Zhang Jianliang Lv Huichen Guo Yonglu Wang Sa Xiao Xinsheng Liu Tags: Article Source Type: research

Streptococcus suis surface-antigen recognition by antibodies and bacterial elimination is influenced by capsular polysaccharide structure
Streptococcus suis is an encapsulated bacterium causing severe diseases in swine. Here, we compared the protective properties of the capsular polysaccharide (CPS) of different S. suis serotypes by using serotype-switched mutants in a mouse model of infection. CPS structure influenced bacterial survival in mice, antibody binding, and antibody-mediated bacterial killing. The CPS of serotypes 3, 4 and 14 allowed more antibody binding and bacterial elimination than the CPS of serotypes 2, 7 and 9. Results suggest that the different CPS structures of S. suis provide varying levels of protection by influencing antigen availabili...
Source: Frontiers in cellular and infection microbiology - July 21, 2023 Category: Microbiology Source Type: research

Viruses, Vol. 15, Pages 1594: First Insight into the Seroepidemiology of Hepatitis E Virus (HEV) in Dogs, Cats, Horses, Cattle, Sheep, and Goats from Bulgaria
alena Baymakova In recent years, hepatitis E virus (HEV) infection has been found to be widespread among different animal species worldwide. In Bulgaria, high HEV seropositivity was found among pigs (60.3%), wild boars (40.8%), and East Balkan swine (82.5%). The aim of the present study was to establish the seroprevalence of HEV among dogs, cats, horses, cattle, sheep, and goats in Bulgaria. In total, 720 serum samples from six animal species were randomly collected: dogs—90 samples; cats—90; horses—180; cattle—180; sheep—90; and goats—...
Source: Viruses - July 21, 2023 Category: Virology Authors: Ilia Tsachev Krasimira Gospodinova Roman Pepovich Katerina Takova Todor Kundurzhiev Gergana Zahmanova Kristin Kaneva Magdalena Baymakova Tags: Article Source Type: research

Linear epicardial cryoablation effects in a porcine model: Lesion characteristics and vascular risk
ConclusionSingle application freezes with an aluminum linear cryoablation probe can create homogeneous ablative lesions over the ventricular myocardium with a maximum depth of approximately 1.0  cm. No large vessel injury occurred with direct lesion application of the LAD; however, small coronary vessels may be at risk.
Source: Journal of Cardiovascular Electrophysiology - July 20, 2023 Category: Cardiology Authors: Justin Hayase, Gregory Fishbein, Vilasinee Rerkpichaisuth, Wei ‐Hsin Chung, Olujimi Ajijola, Kalyanam Shivkumar, Jason S. Bradfield Tags: ORIGINAL ARTICLE Source Type: research

A comparison of histotripsy and percutaneous cryoablation in a chronic healthy swine kidney model
To compare the safety and efficacy of histotripsy with cryoablation in a chronic human-scale normal porcine kidney model.
Source: Journal of Vascular and Interventional Radiology : JVIR - July 20, 2023 Category: Radiology Authors: Allison B. Couillard, Annie M. Zlevor, Timothy J. Ziemlewicz, Meridith A. Kisting, Emily Knott, Annika E. Rossebo, Jim White, Meghan G. Lubner, Lori Mankowski Gettle, J. Louis Hinshaw, Lu Mao, William Stoffregen, John F. Swietlik, Erica Knavel-Koepsel, Li Tags: Laboratory Investigation Source Type: research

Viruses, Vol. 15, Pages 1587: Generation and Efficacy of Two Chimeric Viruses Derived from GPE & minus; Vaccine Strain as Classical Swine Fever Vaccine Candidates
In conclusion, the characteristics of vGPE−/PAPeV Erns and vGPE−/PhoPeV Erns affirmed their properties, as the vGPE− vaccine strain, positioning them as ideal candidates for future development of a CSF marker vaccine.
Source: Viruses - July 20, 2023 Category: Virology Authors: Loc Tan Huynh Norikazu Isoda Lim Yik Hew Saho Ogino Yume Mimura Maya Kobayashi Taksoo Kim Tatsuya Nishi Katsuhiko Fukai Takahiro Hiono Yoshihiro Sakoda Tags: Article Source Type: research

Network Visualization Analysis on MicroRNAs in Infectious Diseases Research Area
CONCLUSIONS: Since 2019, the number of studies on miRNA in infectious illnesses has steadily increased. China and the USA have made tremendous contributions to this field's study. We discovered several deregulated miRNAs, including miR-122, miR-133a, miR-146, miR-155, and miR-370, were described in the context of sepsis and infection using bibliometric methods. Understanding these crucial factors, as well as how research is performed and directed, might lead to a new perspective in the creation of new strategies to manage variable infections in the coming years.PMID:37464988 | PMC:PMC10351212 | DOI:10.5001/omj.2023.71
Source: Topics in HIV Medicine - July 19, 2023 Category: Infectious Diseases Authors: Sevil Alkan Mustafa Serhat Şahinoğlu Havva Yasemin Çinpolat Source Type: research