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Antibiotic resistome and associated bacterial communities in agricultural soil following the amendments of swine manure-derived fermentation bed waste
The objective of this research was to examine the impacts of amending agricultural soil samples with swine manure-derived FBW on microbial communities, mobile genetic elements (MGEs), and ARG profiles over different periods. The study findings indicated that the amendment of swine manure-derived FBW significantly increased the diversity and abundance of ARGs and MGEs during the early stages of amendment, but this effect diminished over time, and after 12 months of FBW amendments, the levels returned to those comparable to control samples. The shift in the bacterial communities played a significant role in shaping the patte...
Source: Environmental Science and Pollution Research International - September 13, 2023 Category: Environmental Health Authors: Zhizhen Pan Zheng Chen Liting Zhu Ricardo David Avell án-Llaguno Bo Liu Qiansheng Huang Source Type: research

Archaeome structure and function of the intestinal tract in healthy and H1N1 infected swine
Conclusions/Take home messageFinally, this study provides the first multi-omics analysis and high-resolution insights into the structure and function of the porcine intestinal tract archaeome during a non-lethal Influenza A virus infection of the respiratory tract, demonstrating significant alterations in archaeal community composition and central metabolic functions.
Source: Frontiers in Microbiology - September 13, 2023 Category: Microbiology Source Type: research

High-dimensional analysis reveals an immune atlas and novel neutrophil clusters in the lungs of model animals with Actinobacillus pleuropneumoniae-induced pneumonia
AbstractDue to the increase in bacterial resistance, improving the anti-infectious immunity of the host is rapidly becoming a new strategy for the prevention and treatment of bacterial pneumonia. However, the specific lung immune responses and key immune cell subsets involved in bacterial infection are obscure.Actinobacillus pleuropneumoniae (APP) can cause porcine pleuropneumonia, a highly contagious respiratory disease that has caused severe economic losses in the swine industry. Here, using high-dimensional mass cytometry, the major immune cell repertoire in the lungs of mice with APP infection was profiled. Various phe...
Source: Veterinary Research - September 13, 2023 Category: Veterinary Research Source Type: research

Longitudinal analysis of differences and similarities in antimicrobial resistance among commensal Escherichia coli isolated from market swine and sows at slaughter in the United States of America, 2013-2019
Int J Food Microbiol. 2023 Sep 7;407:110388. doi: 10.1016/j.ijfoodmicro.2023.110388. Online ahead of print.ABSTRACTThe emergence of antimicrobial resistance in swine enteric bacteria poses a significant public health challenge. Our study evaluated publicly available data collected by the National Antimicrobial Resistance Monitoring System for Enteric Bacteria (NARMS) between 2013 and 2019 at slaughter plants across the United States of America, focusing on commensal E. coli isolated from swine cecal contents originating from two distinct swine production systems: market hogs (n = 2090) and sows (n = 1147). In both producti...
Source: International Journal of Food Microbiology - September 12, 2023 Category: Food Science Authors: Hamid Reza Sodagari Isha Agrawal Setyo Yudhanto Csaba Varga Source Type: research

Photochemical Tissue Bonding of Amnion Allograft Membranes for Peripheral Nerve Repair: A Biomechanical Analysis
Conclusion PTB repairs with commercial noncrosslinked amnion allograft membranes can withstand physiological strain and have comparable performance to repairs with human amnion, which has demonstrated efficacy in vivo. These results indicate the need for further testing of these membranes using in vivo animal model repairs. [...] Thieme Medical Publishers, Inc. 333 Seventh Avenue, 18th Floor, New York, NY 10001, USAArticle in Thieme eJournals: Table of contents  |  Abstract  |  Full text
Source: Journal of Reconstructive Microsurgery - September 11, 2023 Category: Surgery Authors: King, Nicholas C. McGuire, Kalyn R. Bejar-Chapa, Maria Hoftiezer, Yannick A. J. Randolph, Mark A. Winograd, Jonathan M. Tags: Original Article Source Type: research

Detection of resistance and virulence plasmids in Campylobacter coli and Campylobacter jejuni isolated from North Carolina food animal production, 2018-2019
Food Microbiol. 2023 Dec;116:104348. doi: 10.1016/j.fm.2023.104348. Epub 2023 Aug 11.ABSTRACTCampylobacter remains the leading cause of bacterial foodborne illness in the U.S. and worldwide. Campylobacter plasmids may play a significant role in antimicrobial resistance (AMR) and virulence factor distribution, and potentially drive rapid adaptation. C. coli (n = 345) and C. jejuni (n = 199) isolates collected from live cattle, swine, turkey, and chickens, poultry carcasses at production, and retail meat in N.C. were analyzed to determine plasmid prevalence, extrachromosomal virulence and AMR genes, and the phylogeny of asse...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Dawn M Hull Erin Harrel Lyndy Harden Siddhartha Thakur Source Type: research

A recombinant chimeric influenza virus vaccine expressing the consensus H3 hemagglutinin elicits broad hemagglutination inhibition antibodies against divergent swine H3N2 influenza viruses
This study focuses on the hemagglutinin (HA) consensus-based approach towards developing a more broadly protective swine influenza vaccine against various H3 strains circulating in domestic pig populations. By computationally analyzing >1000 swine H3 full-length HA sequences, we generated a consensus H3 and expressed it in the context of influenza A WSN/33 reverse genetics system. The derived recombinant chimeric swine influenza virus with the consensus H3 was inactivated and further evaluated as a potential universal vaccine in pigs. The consensus H3 vaccine elicited broadly active hemagglutination inhibition (HI) anti...
Source: Vaccine - September 9, 2023 Category: Allergy & Immunology Authors: Jieshi Yu Chithra Sreenivasan Zhizhang Sheng Shao-Lun Zhai Jared W Wollman Sisi Luo Chen Huang Rongyuan Gao Zhao Wang Radhey S Kaushik Jane Christopher-Hennings Eric Nelson Ben M Hause Feng Li Dan Wang Source Type: research

Detection of resistance and virulence plasmids in Campylobacter coli and Campylobacter jejuni isolated from North Carolina food animal production, 2018-2019
Food Microbiol. 2023 Dec;116:104348. doi: 10.1016/j.fm.2023.104348. Epub 2023 Aug 11.ABSTRACTCampylobacter remains the leading cause of bacterial foodborne illness in the U.S. and worldwide. Campylobacter plasmids may play a significant role in antimicrobial resistance (AMR) and virulence factor distribution, and potentially drive rapid adaptation. C. coli (n = 345) and C. jejuni (n = 199) isolates collected from live cattle, swine, turkey, and chickens, poultry carcasses at production, and retail meat in N.C. were analyzed to determine plasmid prevalence, extrachromosomal virulence and AMR genes, and the phylogeny of asse...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Dawn M Hull Erin Harrel Lyndy Harden Siddhartha Thakur Source Type: research

Evaluation of dynamics of immune responses and protective efficacy in piglets immunized with an inactivated porcine reproductive and respiratory syndrome vaccine candidate
In this study, we investigated the protective efficacy of an inactivated PRRS virus vaccine candidate and evaluated the differences in PRRSV specific anamnestic response in piglets when challenged with live PRRSV at two different intervals post-immunization. Six-week-old piglets were immunized intramuscularly with an inactivated, Montanide ISA-206 adjuvanted Indian PRRSV isolate, followed by a booster dose at 21 days post-immunization. Homologous live PRRS virus challenge was done on 60 and 180 days post-booster (dpb). We assessed humoral and cell-mediated immune responses at various intervals post-immunization and after c...
Source: Vaccine - September 9, 2023 Category: Allergy & Immunology Authors: Megha Katare Pandey Katherukamem Rajukumar Dhanapal Senthilkumar Subbiah Kombiah Fateh Singh Govindarajulu Venkatesh Manoj Kumar Sangeeta Shrivas Deepali Shrivastava Vijendra Pal Singh Aniket Sanyal Source Type: research

Detection of resistance and virulence plasmids in Campylobacter coli and Campylobacter jejuni isolated from North Carolina food animal production, 2018-2019
Food Microbiol. 2023 Dec;116:104348. doi: 10.1016/j.fm.2023.104348. Epub 2023 Aug 11.ABSTRACTCampylobacter remains the leading cause of bacterial foodborne illness in the U.S. and worldwide. Campylobacter plasmids may play a significant role in antimicrobial resistance (AMR) and virulence factor distribution, and potentially drive rapid adaptation. C. coli (n = 345) and C. jejuni (n = 199) isolates collected from live cattle, swine, turkey, and chickens, poultry carcasses at production, and retail meat in N.C. were analyzed to determine plasmid prevalence, extrachromosomal virulence and AMR genes, and the phylogeny of asse...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Dawn M Hull Erin Harrel Lyndy Harden Siddhartha Thakur Source Type: research

A recombinant chimeric influenza virus vaccine expressing the consensus H3 hemagglutinin elicits broad hemagglutination inhibition antibodies against divergent swine H3N2 influenza viruses
This study focuses on the hemagglutinin (HA) consensus-based approach towards developing a more broadly protective swine influenza vaccine against various H3 strains circulating in domestic pig populations. By computationally analyzing >1000 swine H3 full-length HA sequences, we generated a consensus H3 and expressed it in the context of influenza A WSN/33 reverse genetics system. The derived recombinant chimeric swine influenza virus with the consensus H3 was inactivated and further evaluated as a potential universal vaccine in pigs. The consensus H3 vaccine elicited broadly active hemagglutination inhibition (HI) anti...
Source: Vaccine - September 9, 2023 Category: Allergy & Immunology Authors: Jieshi Yu Chithra Sreenivasan Zhizhang Sheng Shao-Lun Zhai Jared W Wollman Sisi Luo Chen Huang Rongyuan Gao Zhao Wang Radhey S Kaushik Jane Christopher-Hennings Eric Nelson Ben M Hause Feng Li Dan Wang Source Type: research

Evaluation of dynamics of immune responses and protective efficacy in piglets immunized with an inactivated porcine reproductive and respiratory syndrome vaccine candidate
In this study, we investigated the protective efficacy of an inactivated PRRS virus vaccine candidate and evaluated the differences in PRRSV specific anamnestic response in piglets when challenged with live PRRSV at two different intervals post-immunization. Six-week-old piglets were immunized intramuscularly with an inactivated, Montanide ISA-206 adjuvanted Indian PRRSV isolate, followed by a booster dose at 21 days post-immunization. Homologous live PRRS virus challenge was done on 60 and 180 days post-booster (dpb). We assessed humoral and cell-mediated immune responses at various intervals post-immunization and after c...
Source: Vaccine - September 9, 2023 Category: Allergy & Immunology Authors: Megha Katare Pandey Katherukamem Rajukumar Dhanapal Senthilkumar Subbiah Kombiah Fateh Singh Govindarajulu Venkatesh Manoj Kumar Sangeeta Shrivas Deepali Shrivastava Vijendra Pal Singh Aniket Sanyal Source Type: research