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Molecules, Vol. 28, Pages 6496: Investigation of 29 Antimicrobial Compounds in Soil Using Newly Developed UHPLC-MS/MS Method
In this study, an affordable, rugged and simple analytical method has been developed for the determination of twenty-nine antimicrobial compounds from five different classes (tetracyclines, fluoro(quinolones), macrolides, sulfonamides and diaminopirimidines). Liquid–liquid extraction (LLE) with extract filtration combined with ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was the best strategy for the simultaneous determination of all analytes. The developed method was validated according to the Commission Implementing Regulation (EU) 2021/808. The limit of detections (LO...
Source: Molecules - September 7, 2023 Category: Chemistry Authors: Ma łgorzata Gbylik-Sikorska Anna Gajda Monica Felipe-Sotelo Manuela Cani ça Adriana Cabal-Rosel Tanel Tenson Marta Ko řínková Kr õõt Arbo Veljo Kisand Gerhard Rab Martin Brandtner Tags: Article Source Type: research

Viruses, Vol. 15, Pages 1893: Genetic Diversity of Type A Influenza Viruses Found in Swine Herds in Northwestern Poland from 2017 to 2019: The One Health Perspective
In this study, we investigated the genetic diversity of swine influenza viruses (swIAVs) collected from Polish farms. A total of 376 samples were collected from 11 farms. The infection was confirmed in 112 cases. The isolates were subjected to next-generation sequencing (NGS), resulting in 93 full genome sequences. Phylogenetic analysis classified 59 isolates as genotype T (H1avN2g) and 34 isolates as genotype P (H1pdmN1pdm), all of which had an internal gene cassette (IGC) derived from the H1N1pdm09-like strain. These data are consistent with evolutionary trends in European swIAVs. The applied methodology proved to be use...
Source: Viruses - September 7, 2023 Category: Virology Authors: Lukasz Rabalski Maciej Kosinski Piotr Cybulski Tomasz Stadejek Krzysztof Lepek Tags: Article Source Type: research

Coinfection of porcine astrovirus and other porcine viruses in diarrheic pigs in Haryana, India
In this study, 306 rectal swabs from diarrheal pigs of various ages (0-3 weeks, 3-6 weeks, and>6 weeks) were collected from 54 piggery units in different climatic zones in Haryana state, India. These samples were tested for the presence of porcine astrovirus (PAstV), porcine rotavirus group A (PRV-A), and classical swine fever virus (CSFV) by reverse transcription polymerase chain reaction (RT-PCR), and porcine circovirus 2 (PCV-2) by polymerase chain reaction (PCR). Out of the 306 samples tested, 153 (50%), 108 (35.3%), 32 (10.6%), and three (0.9%) tested positive for PAstV, PCV-2, PRV-A, and CSFV, respectively. A sing...
Source: Archives of Virology - September 7, 2023 Category: Virology Source Type: research

Flexible instrument with contact-aided structure and force feedback for endoscopic surgery
CONCLUSIONS: The animal trial conducted in the digestive tract of a live swine proved that the system has the potential for clinical use.PMID:37667568 | DOI:10.1002/rcs.2573
Source: Cancer Control - September 5, 2023 Category: Cancer & Oncology Authors: Chi Zhang Zhongyuan Ping Siyang Zuo Source Type: research

Feasibility of the glue-in-plug technique using a novel liquid embolic material in a swine mode
To evaluate the feasibility of the glue-in-plug (GIP) technique using n-butyl-2-cyanoacrylate ‒Lipiodol (NL)‒iopamidol (NLI) for short-segment embolization in swine.
Source: Journal of Vascular and Interventional Radiology : JVIR - September 5, 2023 Category: Radiology Authors: Akira Ikoma, Tetsuo Sonomura, Nobuyuki Higashino, Kodai Fukuda, Hironobu Ihira, Hiroki Furotani, Masataka Koike, Hirotatsu Sato, Shin-Ichi Murata, Hiroki Minamiguchi Tags: Laboratory Investigation Source Type: research

Viruses, Vol. 15, Pages 1874: HDAC-Specific Inhibitors Induce the Release of Porcine Epidemic Diarrhea Virus via the COPII-Coated Vesicles
In this study, we found that the histone deacetylase (HDAC)-specific inhibitors, trichostatin A (TSA) and sodium butyrate (NaB), facilitate the secretion of infectious PEDV particles without interfering with its assembly. We found that PEDV N protein and its replicative intermediate dsRNA colocalize with coat protein complex II (COPII)-coated vesicles. We also showed that the colocalization of PEDV and COPII is enhanced by the HDAC-specific inhibitors. In addition, ultrastructural analysis revealed that the HDAC-specific inhibitors promote COPII-coated vesicles carrying PEDV virions and the secretion of COPII-coated vesicl...
Source: Viruses - September 4, 2023 Category: Virology Authors: Ying Yang Huan Chen Caisheng Zhang Hyun-Jin Shin Yingjuan Qian Yong-Sam Jung Tags: Article Source Type: research

Viruses, Vol. 15, Pages 1870: Attachment, Entry, and Intracellular Trafficking of Classical Swine Fever Virus
Yidi Guo Classical swine fever virus (CSFV), which is a positive-sense, single-stranded RNA virus with an envelope, is a member of the Pestivirus genus in the Flaviviridae family. CSFV causes a severe and highly contagious disease in pigs and is prevalent worldwide, threatening the pig farming industry. The detailed mechanisms of the CSFV life cycle have been reported, but are still limited. Some receptors and attachment factors of CSFV, including heparan sulfate (HS), laminin receptor (LamR), complement regulatory protein (CD46), MER tyrosine kinase (MERTK), disintegrin, and metalloproteinase domain-containing prot...
Source: Viruses - September 3, 2023 Category: Virology Authors: Xin Guo Maolin Zhang Xiaomin Liu Yannan Zhang Chongyang Wang Yidi Guo Tags: Review Source Type: research

Effective treatment of a broad-host-range lytic phage SapYZU15 in eliminating Staphylococcus aureus from subcutaneous infection
In this study, the antimicrobial susceptibility of 47 S. aureus isolates from three swine farms, two slaughterhouses, and four markets (Yangzhou, China) were evaluated. The biological characteristics of four lytic S. aureus phages were compared and the lytic activity of phage SapYZU15 against MDR S. aureus was assessed using milk, fresh pork and a mouse model of subcutaneous abscess. The results showed that 28 S. aureus isolates (59.6%, 28/47) exhibited multiple antibiotic resistance to at least three different classes of antibiotics. Compared to SapYZU01, SapYZU02, and SapYZU03, SapYZU15 had a shorter latent period (10 mi...
Source: Cell Research - September 2, 2023 Category: Cytology Authors: Hua Wen Wenyuan Zhou Ying Wu Yajie Li Guoqiang Zhu Zhenwen Zhang Xuewen Gu Cuimei Wang Zhenquan Yang Source Type: research

Characterization of a gE/gI/TK gene-deleted pseudorabies virus variant expressing the Cap protein of porcine circovirus type 2d
Comp Immunol Microbiol Infect Dis. 2023 Aug 27;101:102054. doi: 10.1016/j.cimid.2023.102054. Online ahead of print.ABSTRACTPorcine circovirus type 2 (PCV2) plays a key role in the etiology of PCV2-associated disease (PCVAD), and its predominant strain is PCV2d which is not completely controlled by most commercially available vaccines against PCV2a strains. Pseudorabies (PR) caused by pseudorabies virus (PRV) variants re-emerged in Bartha-K61 vaccine-immunized swine herds in late 2011, which brought considerable losses to the global pig husbandry. Therefore, it is significantly important to develop a safe and effective vacc...
Source: Comparative immunology, microbiology and infectious diseases. - August 31, 2023 Category: Allergy & Immunology Authors: Rui-Qin Xu Lin-Qing Wang Hui-Hua Zheng Run-Bo Tian Lan-Lan Zheng Shi-Jie Ma Hong-Ying Chen Source Type: research

Randomised Controlled Trial on Prophylaxis of Mastitis-Metritis-Agalactia Syndrome in Swine using Caulophyllum Logoplex and Lachesis Logoplex
CONCLUSIONS: Prophylaxis with the homeopathic remedies Caulophyllum Logoplex and Lachesis Logoplex in the present study showed neither an improvement in MMA prevention nor an improvement in health parameters or behavioural traits in the present herd of sows.PMID:37652040 | DOI:10.1055/s-0043-1770725
Source: Homeopathy - August 31, 2023 Category: Complementary Medicine Authors: Kathrin B üttner Janna Oelkers Onno Burfeind Joachim Krieter Irena Czycholl Source Type: research

Characterization of a gE/gI/TK gene-deleted pseudorabies virus variant expressing the Cap protein of porcine circovirus type 2d
Comp Immunol Microbiol Infect Dis. 2023 Aug 27;101:102054. doi: 10.1016/j.cimid.2023.102054. Online ahead of print.ABSTRACTPorcine circovirus type 2 (PCV2) plays a key role in the etiology of PCV2-associated disease (PCVAD), and its predominant strain is PCV2d which is not completely controlled by most commercially available vaccines against PCV2a strains. Pseudorabies (PR) caused by pseudorabies virus (PRV) variants re-emerged in Bartha-K61 vaccine-immunized swine herds in late 2011, which brought considerable losses to the global pig husbandry. Therefore, it is significantly important to develop a safe and effective vacc...
Source: Comparative immunology, microbiology and infectious diseases. - August 31, 2023 Category: Allergy & Immunology Authors: Rui-Qin Xu Lin-Qing Wang Hui-Hua Zheng Run-Bo Tian Lan-Lan Zheng Shi-Jie Ma Hong-Ying Chen Source Type: research

Randomised Controlled Trial on Prophylaxis of Mastitis-Metritis-Agalactia Syndrome in Swine using Caulophyllum Logoplex and Lachesis Logoplex
CONCLUSIONS: Prophylaxis with the homeopathic remedies Caulophyllum Logoplex and Lachesis Logoplex in the present study showed neither an improvement in MMA prevention nor an improvement in health parameters or behavioural traits in the present herd of sows.PMID:37652040 | DOI:10.1055/s-0043-1770725
Source: Homeopathy - August 31, 2023 Category: Complementary Medicine Authors: Kathrin B üttner Janna Oelkers Onno Burfeind Joachim Krieter Irena Czycholl Source Type: research

A realistic model for vasectomy reversal training using swine testicles
CONCLUSIONS: The realistic model presented proved to be viable for carrying out vasectomy reversal training, due to its low cost, easy acquisition, and easy handling, and providing similar tissue characteristics to humans.PMID:37646762 | PMC:PMC10470310 | DOI:10.1590/acb383023
Source: Acta Cirurgica Brasileira - August 30, 2023 Category: Surgery Authors: Deivid Ramos Dos Santos Wender de Jesus Pena Corr êa Junior L ívia Guerreiro de Barros Bentes Rafael Silva Lemos Victor Matheus Mendon ça de Araújo Gabrielly Leite Andrade Renan Kleber Costa Teixeira Lu ís Otávio Amaral Duarte Pinto Herick Pampolha Source Type: research