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Viruses, Vol. 15, Pages 1845: Establishment of a Dual-Antigen Indirect ELISA Based on p30 and pB602L to Detect Antibodies against African Swine Fever Virus
In conclusion, we established a detection method with low cost, streamlined production process, and fewer instruments. It provides a new method for the serological diagnosis of ASF.
Source: Viruses - August 30, 2023 Category: Virology Authors: Lei Zhou Jinxing Song Mengxiang Wang Zhuoya Sun Junru Sun Panpan Tian Guoqing Zhuang Angke Zhang Yanan Wu Gaiping Zhang Tags: Article Source Type: research

Isolation and pathogenicity of porcine circovirus type 2 in mice from Guangxi province, China
Porcine circovirus type 2 (PCV2), a member of the genus Circovirus and family Circoviridae, is a closed, small, circular, and single-stranded DNA virus, and it is a crucial swine pathogen of porcine circovirus-as...
Source: Virology Journal - August 29, 2023 Category: Virology Authors: Qiulin Jiao, Liuyue Yang, Xiangzu Liu, Yanwen Wen, Linxing Tian, Ping Qian, Huanchun Chen and Xiangmin Li Tags: Research Source Type: research

African swine fever virus MGF360-9L promotes viral replication by degrading the host protein HAX1
In conclusion, this study has enriched our understanding of the regulatory networks between ASFV proteins and host proteins and provided a reference for investigation into the pathogenesis and immune escape mechanism of ASFV.PMID:37640268 | DOI:10.1016/j.virusres.2023.199198
Source: Virus Research - August 28, 2023 Category: Virology Authors: Jinke Yang Bo Yang Yu Hao Xijuan Shi Xing Yang Dajun Zhang Dengshuai Zhao Wenqian Yan Lingling Chen Xintian Bie Guohui Chen Zixiang Zhu Dan Li Chaochao Shen Guoli Li Xiangtao Liu Haixue Zheng Keshan Zhang Source Type: research

Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems
Glaesserella parasuis is the causative agent of Gl ässer’s disease in pigs. Serotyping is the most common method used to type G. parasuis isolates. However, the high number of non-typables (NT) and low discriminat...
Source: BMC Veterinary Research - August 28, 2023 Category: Veterinary Research Authors: Robert Mugabi, Ana Paula S. Poeta Silva, Xiao Hu, Marcelo Gottschalk, Virginia Aragon, Nubia R. Macedo, Orhan Sahin, Perry Harms, Rodger Main, Alexander W. Tucker, Ganwu Li and Maria J. Clavijo Tags: Research Source Type: research

The pathophysiological impact of intra-abdominal hypertension in pigs
by Robert Wise, Reitze Rodseth, Ester P árraga-Ros, Rafael Latorre, Octavio López Albors, Laura Correa-Martín, Francisco M. Sánchez-Margallo, Irma Eugenia Candanosa-Aranda, Jan Poelaert, Gregorio Castellanos, Manu L. N. G. Malbrain BackgroundIntra-abdominal hypertension and abdominal compartment syndrome are common with clinically significant consequences. We investigated the pathophysiological effects of raised IAP as part of a more extensive exploratory animal study. The study design included both pneumoperitoneum and mechanical intestinal obstruction models. MethodsForty-nine female swine were divided into six group...
Source: PLoS One - August 28, 2023 Category: Biomedical Science Authors: Robert Wise Source Type: research

Characteristics and genomic epidemiology of colistin-resistant Enterobacterales from farmers, swine, and hospitalized patients in Thailand, 2014 –2017
Colistin is one of the last resort therapeutic options for treating carbapenemase-producing Enterobacterales, which are resistant to a broad range of beta-lactam antibiotics. However, the increased use of coli...
Source: BMC Infectious Diseases - August 28, 2023 Category: Infectious Diseases Authors: Adhiratha Boonyasiri, Lauren M. Brinkac, Elita Jauneikaite, Richard C. White, Chris Greco, Chakkraphong Seenama, Teerawit Tangkoskul, Kevin Nguyen, Derrick E. Fouts and Visanu Thamlikitkul Tags: Research Source Type: research

Identification of neutralizing epitopes on the D/A domain of the E2 glycoprotein of classical swine fever virus
Virus Res. 2023 Aug 24:199209. doi: 10.1016/j.virusres.2023.199209. Online ahead of print.ABSTRACTClassical swine fever virus (CSFV) shares high antigenic homology with other members of the genus Pestivirus. Because several pestivirus species can also infect swine, eliciting cross-reactive antibodies, it is important to define CSFV-specific epitopes for the differential diagnosis of classical swine fever (CSF) by serology. For this purpose, epitope mapping of seven monoclonal antibodies (mAbs), recognizing sites on the D/A domain of glycoprotein E2, was performed using recombinant expressed antigenic domains and mutants of...
Source: Virus Research - August 26, 2023 Category: Virology Authors: Yu-Liang Huang Denise Meyer Alexander Postel Kuo-Jung Tsai Hsin-Meng Liu Chia-Huei Yang Yu-Chun Huang Hui-Wen Chang Ming-Chung Deng Fun-In Wang Paul Becher Helen Crooke Chia-Yi Chang Source Type: research

Real-Time Measurements of  Oral Mucosal Carbon Dioxide (POMCO2) Reveals an Inverse Correlation With Blood Pressure in a Porcine Model of Coagulopathic Junctional Hemorrhage
CONCLUSIONS: Despite the logical appeal of measuring noninvasive tissue CO2 measurement as a surrogate for gastrointestinal perfusion, prior studies have only reported snapshots of this readout. The present investigation shows real-time longitudinal measurement of POMCO2 to confirm that MAP inversely correlates to POMCO2 in the face of coagulopathy. The simplicity of measuring POMCO2 in real time can provide an additional practical option for military or civilian medics to monitor trends in hypoperfusion during hemorrhagic shock.PMID:37632757 | DOI:10.1093/milmed/usad336
Source: Military Medicine - August 26, 2023 Category: International Medicine & Public Health Authors: Biebele Abel Kimberly A Gerling John A Mares Justin Hutzler Irvin Pierskalla Jim Hays Brandon Propper Joseph M White David M Burmeister Source Type: research

Viruses, Vol. 15, Pages 1816: One-Step Assembly of a PRRSV Infectious cDNA Clone and a Convenient CRISPR/Cas9-Based Gene-Editing Technology for Manipulation of PRRSV Genome
anrong Zhou Porcine reproductive and respiratory syndrome (PRRS) has been a persistent challenge for the swine industry for over three decades due to the lack of effective treatments and vaccines. Reverse genetics systems have been extensively employed to build rapid drug screening platforms and develop genetically engineered vaccines. Herein, we rescued recombinant PRRS virus (rPRRSV) WUH3 using an infectious cDNA clone of PRRSV WUH3 acquired through a BstXI-based one-step-assembly approach. The rPRRSV WUH3 and its parental PRRSV WUH3 share similar plaque sizes and multiple-step growth curves. Previously, gene-editing...
Source: Viruses - August 26, 2023 Category: Virology Authors: Hejin Zhang Kaiqi Duan Yingbin Du Shaobo Xiao Liurong Fang Yanrong Zhou Tags: Article Source Type: research